After a lower-limb amputation, most individuals face major adjustments in their psychological, physical, and emotional lives. Their sexuality is expected to be affected. Sexual function of two convenience samples of 1) lower-limb amputees (n = 51) and 2) nonamputees (n = 38) of similar age range (mean, 59 years) was measured using the Changes in Sexual Functioning Questionnaire Short Form (CSFQ-14) instrument. This study examined the associations between demographic factors and comorbidities, such as diabetes mellitus, peripheral vascular disease (PVD), and hypertension on one side and sexual function on the other. The results did not reveal any reduction in sexual functioning solely due to an amputation. Among the comorbidities, the presence of diabetes mellitus in the participants was the only predictor of reduced sexual functioning. This finding was significant only in the amputee group.