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Journal of Pediatric Hematology/Oncology:
doi: 10.1097/MPH.0b013e31827b12ab
Original Articles

Anemia Associated With Acute Infection in Children: An Animal Model

Ballin, Ami MD*,†; Hussein, Aeed PhD; Vaknine, Hananya MD§; Senecky, Yehudah MD; Avni, Yona MD; Schreiber, Letizia MD§; Tamary, Hannah MD; Boaz, Mona PhD#

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Abstract

Background: We constructed an animal model to examine the possibility that erythrophagocytosis may contribute to decreased hemoglobin (Hgb) levels in acute infection in mice.

Methods: BALB/c mice weighing 20 to 25 g were injected (intraperitoneally) with lipopolysaccharide (LPS) (Escherichia coli serotype) of some concentrations. Control mice were injected intraperitoneally with saline (0.5 mL). Two and 4 hours after LPS administration, mice were bled (0.25 mL) for complete blood count measures and tumor necrosis factor-α and interleukin-6 levels. The mice were then killed, and their spleen, liver, and bone marrow were examined microscopically for erythrophagocytosis.

Results: After LPS administration, mouse Hgb and hematocrit levels dropped significantly. At 4 hours after LPS injection, all Hgb and hematocrit concentrations were found to be significantly lower compared with that of controls (P=0.002 and 0.001, respectively). Significantly increased concentrations of tumor necrosis factor-α and interleukin-6 were evident after LPS injection. Prominent hepatic erythrophagocytosis was observed in the LPS-injected mice compared with controls. A significant across-group difference was observed at 4 hours, driven by significantly higher values in group 500 mcg versus controls (P=0.005) and 100 mcg (P=0.025). A significant increase in erythrophagocytes was observed at 2 to 4 hours in the 500 mcg LPS group (P=0.044).

Conclusions: Erythrophagocytosis may play a role in anemia associated with acute infection in mice.

© 2013 Lippincott Williams & Wilkins, Inc.

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