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Quantitative RT-PCR Analysis of the MOZ-CBP Fusion Transcript in Therapy-related Acute Myeloid Leukemia With t(8;16)(p11;p13)

Fujiki, Atsushi MD; Imamura, Toshihiko MD; Furutani, Akiyo MD; Hatano, Waka MD; Asai, Daisuke MD; Hirashima, Yoshifumi MD; Miyachi, Mitsuru MD; Tamura, Shinichi MD; Tsuchiya, Kunihiko MD; Iehara, Tomoko MD; Ishida, Hiroyuki MD; Yoshihara, Takao MD; Hosoi, Hajime MD

Journal of Pediatric Hematology/Oncology: July 2012 - Volume 34 - Issue 5 - p 402–405
doi: 10.1097/MPH.0b013e318238818f
Clinical and Laboratory Observations

We developed a real time reverse transcriptase polymerase chain reaction (RT-PCR) assay system for detecting the MOZ-CBP fusion transcript and used it to monitor minimal residual disease (MRD) status in a patient with therapy related acute myeloid leukemia (t-AML) harboring t(8;16)(p11;p13). Expression of the MOZ-CBP fusion transcript was determined by RT-PCR analysis of the patient’s bone marrow at the time of diagnosis. Thereafter, real time RT-PCR was used to evaluate MRD levels throughout the entire course of treatment. The sensitivity of quantitative RT-PCR for the MOZ-CBP fusion transcript was 10−5. Below this level, MRD was classified as negative. Real time RT-PCR of the bone marrow after induction therapy showed the reduction of MOZ-CBP transcript to approximately 10−3 level when compared to the diagnostic sample. MRD was classified as negative (<10−5 compared with that in the bone marrow at diagnosis) after 5 courses of chemotherapy, a level that was maintained post-allo-hematopoietic stem cell transplantation. Real time RT-PCR of the MOZ-CBP transcript is a useful tool for assessing MRD status for a patient with therapy related acute myeloid leukemia who was initially predicted to have a poor prognosis.

*Department of Pediatrics, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Kyoto

Department of Pediatrics, Matsushita Memorial Hospital, Moriguchi, Japan

This work was supported by a grant from the Ministry of Education, Sports, Science and Technology.The authors declare no conflict of interest.

Reprints: Toshihiko Imamura, MD, Department of Pediatrics, Kyoto Prefectural University of Medicine, Graduate School of Medical Science, 465 Kajii-cho, Hirokoji, Kamigyo-ku, Kyoto, Japan 602-8566 (e-mail: imamura@koto.kpu-m.ac.jp).

Received March 28, 2011

Accepted September 19, 2011

Copyright © 2012 Wolters Kluwer Health, Inc. All rights reserved.