Share this article on:

Effects of Infant Formula With Human Milk Oligosaccharides on Growth and Morbidity: A Randomized Multicenter Trial

Puccio, Giuseppe*; Alliet, Philippe; Cajozzo, Cinzia*; Janssens, Elke; Corsello, Giovanni*; Sprenger, Norbert||; Wernimont, Susan; Egli, Delphine§; Gosoniu, Laura||; Steenhout, Philippe

Journal of Pediatric Gastroenterology & Nutrition: April 2017 - Volume 64 - Issue 4 - p 624–631
doi: 10.1097/MPG.0000000000001520
Original Articles: Nutrition

Objectives: The aim of the study was to evaluate the effects of infant formula supplemented with 2 human milk oligosaccharides (HMOs) on infant growth, tolerance, and morbidity.

Methods: Healthy infants, 0 to 14 days old, were randomized to an intact-protein, cow's milk–based infant formula (control, n = 87) or the same formula with 1.0 g/L 2′fucosyllactose (2′FL) and 0.5 g/L lacto-N-neotetraose (LNnT) (test, n = 88) from enrollment to 6 months; all infants received standard follow-up formula without HMOs from 6 to 12 months. Primary endpoint was weight gain through 4 months. Secondary endpoints included additional anthropometric measures, gastrointestinal tolerance, behavioral patterns, and morbidity through age 12 months.

Results: Weight gain was similar in both groups (mean difference [95% confidence interval] test vs control: −0.30 [−1.94, 1.34] g/day; lower bound of 95% confidence interval was above noninferiority margin [−3 g/day]). Digestive symptoms and behavioral patterns were similar between groups; exceptions included softer stool (P = 0.021) and fewer nighttime wake-ups (P = 0.036) in the test group at 2 months. Infants receiving test (vs control) had significantly fewer parental reports (P = 0.004–0.047) of bronchitis through 4 (2.3% vs 12.6%), 6 (6.8% vs 21.8%), and 12 months (10.2% vs 27.6%); lower respiratory tract infection (adverse event cluster) through 12 months (19.3% vs 34.5%); antipyretics use through 4 months (15.9% vs 29.9%); and antibiotics use through 6 (34.1% vs 49.4%) and 12 months (42.0% vs 60.9%).

Conclusions: Infant formula with 2′FL and LNnT is safe, well-tolerated, and supports age-appropriate growth. Secondary outcome findings showing associations between consuming HMO-supplemented formula and lower parent-reported morbidity (particularly bronchitis) and medication use (antipyretics and antibiotics) warrant confirmation in future studies.

*Dipartimento Materno Infantile, AOUP “Paolo Giaccone,” Università di Palermo, Palermo, Italy

Department of Paediatrics, Jessa Hospital, Hasselt, Belgium

Nestlé Nutrition R&D, King of Prussia, PA

§Nestlé Nutrition, Vevey

||Nestlé Research Center, Nestec Ltd, Lausanne

Nestlé Health Science, Epalinges, Switzerland.

Address correspondence and reprint requests to Giuseppe Puccio, MD, Dipartimento Materno Infantile, AOUP “Paolo Giaccone,” Università di Palermo, Via Alfonso Giordano 3, 90127 Palermo, Italy (e-mail:

Received 29 July, 2016

Accepted 13 January, 2017

Supplemental digital content is available for this article. Direct URL citations appear in the printed text, and links to the digital files are provided in the HTML text of this article on the journal's Web site ( registration number: NCT01715246.

This study was sponsored by Nestec Ltd. The infant formula evaluated in this study was prepared for the purpose of the clinical trial by Nestlé Product Technology Center Konolfingen and was provided free of charge.

Portions of this study were presented in abstract form at the 3rd International Conference on Nutrition and Growth, Vienna, Austria, March 17–19, 2016.

Nestlé provided grants and nonfinancial support to AOUP “Paolo Giaccone,” Palermo, Italy, and to the Department of Paediatrics, Jessa Hospital, Hasselt, Belgium. G.P. and C.C. have also worked on other studies sponsored by Nestlé. N.S. and L.G. are current employees of Nestlé Research Center, Nestec Ltd. S.W. is a former employee of Nestlé Nutrition. D.E. is a current employee of Nestlé Nutrition. P.S. is a current employee of Nestlé Health Sciences. The remaining authors report no conflicts of interest.

This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

What Is Known

* Human milk contains structurally diverse oligosaccharides, which may support gastrointestinal and immune functions in breast-fed infants.

* Numerous studies have reported a lower incidence of infections, including respiratory tract infections, in breast-fed infants compared with those fed infant formula.

What Is New

* Infant formula supplemented with 2′fucosyllactose and lacto-N-neotetraose, 2 oligosaccharides commonly found in human milk, is safe, well-tolerated and supports age-appropriate growth.

* Secondary outcome findings of lower morbidity (particularly bronchitis) and medication use (antipyretics and antibiotics) were reported in infants fed supplemented formula, associations that warrant confirmation in future studies.

Human milk contains an abundance of structurally diverse oligosaccharides, known collectively as human milk oligosaccharides (HMOs), which represent the third largest solid component of human milk after lactose and lipids (1,2). Three classes of oligosaccharides can be distinguished in human milk: neutral fucosylated (including 2′fucosyllactose, 2′FL), neutral nonfucosylated (including lacto-N-neotetraose, LNnT), and acidic (including 3′sialyllactose, 3′SL, and 6′sialyllactose, 6′SL) (3–5). In contrast, cow's milk contains relatively low oligosaccharide content with limited structural diversity (6). Although the types and concentrations of HMOs vary considerably among lactating women and over time, 2 that are commonly found in abundance in human milk are 2′FL and LNnT. Levels of 2′FL typically range from 1.1 to 4.3 g/L in mature milk based on pooled data from secretors and nonsecretors (3,5,7–17). Levels of LNnT in mature milk typically range from 0.1 to 0.6 g/L (5,9,10,12,15,18–20), with higher levels more commonly observed within the first month of lactation (12).

Emerging research has suggested the importance of HMOs in enhancing the development of the intestinal microbiota and supporting immune protection in breast-fed infants (21,22). Both 2′FL and LNnT promote the growth of Bifidobacterium species in preclinical models (23,24). In addition to this, in mice infected with adherent-invasive Escherichia coli (AIEC), 2′FL was effective in reducing colonization by AIEC, modulating AIEC-induced CD14 expression, and attenuating inflammation associated with E. coli invasion (25). In healthy infants, the relative absorption of 2′FL was similar among breast-fed infants and those fed formula supplemented with 2′FL (26), suggesting that 2′FL and possibly other HMOs may exert effects beyond the gastrointestinal (GI) tract (27,28). These findings, in conjunction with the documented differences in oligosaccharide composition between human and bovine milk, support the rationale for supplementing cow's milk–based infant formulas with 2′FL and LNnT. Although the safety of HMOs has been previously established in preclinical studies (29,30), clinical data regarding the effects of HMO supplementation on infant growth and tolerability are extremely limited (26). Therefore, the primary objective of this study was to evaluate the growth of infants fed a new cow's milk–based formula supplemented with 2′FL and LNnT. Secondary objectives included the evaluation of anthropometric measures, GI tolerance, and behavioral patterns, as well as morbidity through age 12 months.

Back to Top | Article Outline


This was a multicenter, randomized, double-blind trial of 2 parallel groups of formula-fed infants who were enrolled at ≤14 days of age. Formula-fed infants who met the eligibility criteria were randomized equally to receive either control or test formula through 6 months of age. Randomization was carried out using a permuted block algorithm with Medidata Balance (New York, NY) and was stratified by infant sex and delivery method (vaginal or cesarean) to ensure balance of infant sex and delivery mode between groups. Parents/caregivers (hereafter “parents”), investigators and study support staff were blinded to the study formulas; formulas were coded by the manufacturer (Nestlé Product Technology Center, Konolfingen, Switzerland) using a single nonspeaking code per formula group.

The control formula was an intact protein, cow's milk–based, whey-predominant infant formula with long-chain polyunsaturated fatty acids (67 kcal/100 mL reconstituted formula; 1.8 g protein/100 kcal with a whey:casein ratio of 70%:30%). The test formula was identical to control except for the addition of 2 HMOs (Glycom A/S, Kongens Lyngby, Denmark) providing 1.0 g 2′FL and 0.5 g LNnT per liter of reconstituted formula. Although not directly measured, the osmolarities of the 2 study formulas were likely similar (or even slightly lower in the test formula) because HMOs, which replaced the same amount of lactose in the test formula, have higher molecular weight than lactose. The test and control formulas were distributed at study visits until age 6 months, when infants in both formula groups were switched to an intact protein, cow's milk–based, follow-up formula without HMOs for feedings through age 12 months. Parents were advised to feed the study formulas to their infants as they deemed appropriate, based on the infant's appetite, age, and weight. Complementary foods were allowed beginning at age 4 months.

The study was conducted between October 2012 and July 2015 in the Dipartimento Materno Infantile AOUP “Paolo Giaccone,” Università di Palermo, Palermo, Italy, and in the Department of Paediatrics at Jessa Hospital in Hasselt, Belgium. The study was approved by the ethical committees of both hospitals. Trial conduct complied with the Declaration of Helsinki and the International Conference on Harmonization guidelines for Good Clinical Practice. Informed consent was obtained from the parent or legal guardian of each infant before enrollment.

Back to Top | Article Outline


We enrolled healthy infants using the following inclusion criteria: ≤14 days of age; gestation 37 to 42 weeks; birth weight 2500 to 4500 g; mothers had independently elected, before enrollment, not to breast-feed; exclusive formula-feeding at time of enrollment; and parent/legal guardian informed consent. Exclusion criteria were congenital illness or malformation that could affect growth; significant prenatal and/or serious postnatal disease before enrollment; minor parent(s); parents not expected to comply with study procedures; and current or previous participation in another clinical trial.

Back to Top | Article Outline

Study Visits

Infants completed a baseline visit at ≤14 days of age, followed by visits at 1, 2, 3, 4, 6, and 12 months of age. At the baseline visit, demographic information and participant characteristics were collected, a clinical examination was performed and anthropometrics (weight, length, and head circumference) were obtained. At each subsequent visit, a clinical examination was performed, anthropometrics were obtained, and digestive tolerance, behavioral patterns, and formula intake were assessed using a paper diary completed by the parent reflecting 3 consecutive days before the visit. Parents also provided information on illness symptoms and medication use in the diary, which was reviewed and confirmed by the physician at each study visit.

Back to Top | Article Outline

Outcome Measures

The primary outcome was weight gain (g/day) between enrollment and age 4 months (calculated as the difference in infant weight between the baseline visit and the visit at age 4 months, divided by the number of days between these 2 visits), as recommended in guidelines from the American Academy of Pediatrics Task Force on Clinical Testing of Infant Formulas (31). The period from 0 to 4 months represents the timeframe when infant formula is used as the sole source of nutrition. Secondary outcomes included other anthropometric measures (weight, length, body mass index [BMI], head circumference, and corresponding z scores), digestive tolerance (flatulence, spitting-up, and vomiting), stool characteristics (stool consistency and frequency), behavior patterns (restlessness, colic, and nighttime awakenings), formula intake, and morbidity (parent-reported adverse events [AEs] and concomitant medications).

Detailed information on outcome measurement procedures is provided in Supplemental Digital Content 1 ( Briefly, anthropometrics were measured by trained study personnel using standard procedures; z scores were calculated using the WHO 2006 Child Growth Standards (32). Diaries completed by parents in the 3-day period before each postbaseline study visit provided information on number of stools per day and stool consistency (1 = hard, 7 = watery; stool images and descriptions provided to parents). Parents were also asked to respond to a series of structured questions in the diary designed to assess the frequency with which their child exhibited symptoms related to digestive tolerance and behavioral patterns (ie, flatulence, spitting-up, vomiting, colic, restless and irritable, and waking up during the night) by selecting one of the response options (ie, never, sometimes, or often). During the initial baseline visit, parents were instructed on how and when to fill out the diary, and at each of the subsequent visits, the investigators reviewed the diary with the parents to verify the completeness and accuracy of the diary entries.

The diary also included a grid for the parent to record any illness symptoms including fever. Although parents were not expected to diagnose specific illnesses, they were asked to convey in the diary any illness diagnoses provided to them by either the child's primary care physician or the study physician when the child was ill. Parents also could record in the grid any hospitalizations (reason, duration) and intake of medications since the prior visit, and the associated start and stop dates. The information recorded in the diary by the parent was reviewed and verified by the study physician at each study visit before being recorded in the study database as reported AEs or reported medication use. AEs were then coded and categorized by a single physician who was not involved in study conduct using the Medical Dictionary for Regulatory Activities (MedDRA) System, Organ, and Class (SOC) categories as well as the Preferred Terms (PTs) within each SOC category. Several PTs within the “Infections and Infestations” SOC category were identified a priori as being of particular interest, including upper respiratory tract infection, pyrexia, rhinitis, bronchitis, bronchiolitis, otitis media, pharyngitis and gastroenteritis. In addition to this, 3 AE clusters were identified: upper respiratory tract infection, lower respiratory tract infection, and otitis/ear infection (see Supplemental Digital Content 1,, for PTs included in each AE cluster). Similarly, recorded medications were coded and categorized into groups, including antibiotics, antipyretics, and gastroesophageal reflux disease medications. Data related to AEs and medication use were expressed as the proportion of infants in each group with at least 1 episode of the specific AE or medication use.

Back to Top | Article Outline

Statistical Analysis

The sample size was determined according to guidelines from the American Academy of Pediatrics Task Force on Clinical Testing of Infant Formulas (31). Specifically, power calculations were performed using the software package R version 2.13.2 (2011; The R Foundation for Statistical Computing, Vienna, Austria). Assuming 80% power, α = 0.025 for the 1-sided weight gain noninferiority comparison between groups, a noninferiority margin of −3 g/day (31), and a standard deviation of 6.1 g/day (33), a total of 66 infants were needed in each formula-fed group. Enrollment of approximately 88 infants per group was planned to account for a 25% dropout rate.

The primary outcome (weight gain, g/day from baseline to 4 months) was analyzed using an analysis of covariance model adjusted for multiple covariates/factors including baseline weight, treatment, sex, and center. Missing postbaseline weight gain was imputed using the respective treatment visit mean by sex. Adjusted mean and standard error (SE) of weight gain was reported. Weight gain in the test group was considered noninferior to control if the lower bound of the 2-sided 95% confidence interval (CI) for the difference between groups (test minus control) was above the noninferiority margin of −3 g/day (31). The primary outcome was analyzed in both the per-protocol (PP) and intention-to-treat (ITT) populations. The PP population included infants with none of the following major protocol violations before age 4 months: being hospitalized for >3 consecutive days 1 week before age 4 months, being off study formula for ≥3 consecutive days, and taking ≥4 teaspoons of complementary food per day. The ITT population included all infants randomized to study formula.

Secondary outcomes were evaluated in the ITT population, without adjustment for multiple testing. Anthropometrics were analyzed using a mixed-effect model repeated measures approach adjusted for multiple covariates/factors and their interactions including baseline anthropometric assessments, sex, center, visit, treatment, sex × visit, and sex × treatment. The Cochran-Mantel-Haenszel test was used to evaluate the linear association between study formula and digestive tolerance, behavioral patterns, and stool consistency. Stool consistency was also compared between groups using a Student t test at each visit. Stool frequency was analyzed using the negative binomial generalized linear model. Morbidity outcomes were analyzed using the Fisher exact test and reported as odds ratio (OR) and 95% CI (See Supplemental Digital Content 2,, for additional details on statistical analyses).

Back to Top | Article Outline


A total of 175 infants were enrolled (95 from the site in Belgium and 80 from the site in Italy), with 87 and 88 randomized to receive the control and test formulas, respectively (Fig. 1). Forty-four (25.1%) infants (20 in control; 24 in test) withdrew before the primary outcome assessment (4-month follow-up). The dropout rate was comparable between groups and was accounted for in the sample size calculation. The most common reason for discontinuation was an AE (n = 11 in control; n = 12 in test). Other reasons for discontinuation before 4 months included parent/guardian request (n = 3 in control; n = 6 in test); lost to follow-up/missing (n = 5 in control; n = 6 in test); and other (n = 1 in control; n = 0 in test). Infant baseline characteristics and anthropometrics were comparable between groups (Table 1).

Back to Top | Article Outline


In the PP population, adjusted mean (SE) weight gain through age 4 months was 29.84 (0.60) g/day for infants fed the test formula and 30.15 (0.58) g/day for those fed control (Table 2). Mean difference (95% CI) in weight gain between groups was −0.30 (−1.94, 1.34) g/day, with the lower limit of the 95% CI above the predefined noninferiority margin of −3 g/day, indicating similar weight gain in the 2 groups. Results were similar in the ITT population. Mean weight, length, head circumference, and BMI were not significantly different between groups at any study visit (see Supplemental Digital Content 3, Likewise, mean weight-for-age, length-for-age, head circumference-for-age, and BMI-for-age z scores (Fig. 2) did not differ significantly between the groups at any study visit and tracked closely with the WHO growth standards.

Back to Top | Article Outline

Formula Intake and Tolerance

Mean daily formula intake was similar between groups at all study visits. At age 4 months, mean intake was 908 mL in the test group and 929 mL in the control group (P = 0.54). GI symptoms, including flatulence, spitting-up and vomiting, were not significantly different at any study visit between the groups, indicating no association between type of formula and digestive tolerance. No differences were observed between the groups in the proportion of stools in each consistency category. Comparison of mean ± SE stool consistency ratings showed that the test group had a tendency toward softer stools at 1 month (5.9 ± 0.2 vs 5.5 ± 0.2, P = 0.064) and had significantly softer stools at 2 months (6.1 ± 0.1 vs 5.7 ± 0.2, P = 0.021); no significant differences were observed at other visits. No significant differences in stool frequency were reported at any visit.

Back to Top | Article Outline

Behavioral Patterns

Parent-reported infant behavioral patterns including restlessness/irritability and colic were similar in the test and control groups at each study visit, although among the subgroup of infants delivered by cesarean section, colic at 4 months was reported less frequently in the test group (P = 0.035). Specifically, among cesarean-born infants, colic was reported as “never” and “sometimes” in 96% and 4%, respectively, in the test group compared to 74% and 26% in the control group. In addition to this, at 2 months, nighttime awakenings were reported less frequently in the test group (P = 0.036), with parents reporting “never,” “sometimes,” and “often” in 27%, 69%, and 4% of infants in the test group, compared with 25%, 57%, and 18% in the control group. This difference, however, did not persist after age 2 months.

Back to Top | Article Outline


The overall percentage of infants who had at least 1 reported AE was similar in both groups during the 4-month exclusive feeding period (test: 84.1%; control 90.8%). At 4 months, at least 1 serious AE was reported in 6 infants (6.8%) in the test group and 10 infants (11.5%) in the control group. Only 1 infant experienced an AE (cow's milk-protein allergy) considered to be related to the study formula (infant was assigned to test). There were no statistically significant differences between groups in the cumulative incidence of reported AEs when analyzed by SOC categories. The percentage of infants who reported at least 1 AE in the SOC category of “Infections and Infestations” from 0 to 12 months was, however, numerically lower in the test group, a difference that approached statistical significance (69.3% vs 82.8%, OR 0.47, 95% CI 0.21–1.02, P = 0.051). No statistically significant differences were observed between groups in other SOC categories.

Regarding the a priori-identified AEs of interest within the “Infections and Infestations” SOC category, as shown in Figure 3, infants receiving test had significantly fewer reports of bronchitis through 4, 6, and 12 months, and the AE cluster of lower respiratory tract infection through 12 months. Similar statistically significant differences between the test and control groups were observed in the subgroup of infants born by cesarean section for bronchitis through 12 months (3.1% vs 34.4%, OR 0.06, 95% CI 0.00–0.50, P = 0.003) and lower respiratory tract infection through 6 (6.3% vs 28.1%, OR 0.17, 95% CI 0.02–0.96, P = 0.043) and 12 months (12.5% vs 40.6%, OR 0.21, 95% CI 0.04–0.83, P = 0.022). Fewer reports of the AE cluster of otitis/ear infection were observed in the test group through 12 months, but the difference between groups did not reach statistical significance (6.8% vs 12.6%, P = 0.213). There were no other statistically significant findings for the reported incidences of AE PTs or AE clusters. Infants receiving test had significantly fewer reports of the use of antipyretics through 4 months; however, this difference was not statistically significant at 6 or 12 months (Fig. 3). Infants receiving test also had significantly fewer reports of antibiotic use through 6 and 12 months.

Back to Top | Article Outline


To our knowledge, this is the first randomized, controlled trial of infant formula supplemented with both 2′FL (1 g/L) and LNnT (0.5 g/L), 2 abundant oligosaccharides in breast milk that collectively represent ∼37% of total HMOs (34). We found that compared with infants fed the control formula without HMOs, those fed the test formula: manifested similar age-appropriate growth, had indications of improved GI comfort in the first few months of age, and had lower rates of parent-reported morbidities related to lower respiratory tract infections as well as antipyretic and antibiotic use. As the study was powered for the primary outcome (weight gain from 0 to 4 months of age), the findings related to secondary outcomes warrant confirmation in future studies.

Back to Top | Article Outline

Growth and Tolerance

The study demonstrated comparable weight gain in the test and control groups and growth tracked closely with the WHO growth standards through 12 months of age. The study also showed that test formula supplemented with 1 g/L 2′FL and 0.5 g/L LNnT is well tolerated. Interestingly, infants fed the test formula tended to have softer stools at 1 month of age and had significantly softer stools at age 2 months. Although these differences did not persist at later time points, the findings highlight the beneficial effect of HMOs on stool characteristics during the first few months of life when the neonatal GI tract undergoes profound growth and functional maturation (35). Moreover, the proportion of parents reporting that their infants woke up “often” at night was lower in the test group at 2 months of age, although this finding did not persist at later time points. The lack of statistical significance for this endpoint after 2 months may be attributable to inadequate power. In addition to this, among cesarean-born infants, parents reported less colic in the test group at age 4 months. Collectively, these findings suggest that infant formula supplemented with 1 g/L 2′FL and 0.5 g/L LNnT may confer improved GI comfort, although further confirmatory studies are needed.

Back to Top | Article Outline

Parent-reported Morbidities and Medication Use

The safety of the test formula is supported by a similar incidence of total reported AEs in the test and control groups. Importantly, we observed lower rates of respiratory tract–related morbidity outcomes among infants fed formula with 2′FL and LNnT compared with those fed the control formula. We also observed a lower likelihood of reporting the use of antipyretic and antibiotic medications in the test group. Although these secondary outcome findings showing associations between HMO-supplemented formula and lower parent-reported morbidity and medication use warrant confirmation in future studies, accumulating evidence (36–40) showing both local anti-adhesion and systemic immunomodulatory effects of HMOs provides mechanistic support for an effect on respiratory tract–related morbidities. Specifically, HMOs rinse the laryngopharyngeal region during oral consumption and could potentially reduce pathogen adhesion locally at the entrance to the upper respiratory tract (41). 2′FL was shown to inhibit the binding of pathogens such as Pseudomonas aeruginosa to respiratory cells (36). LNnT was shown to reduce pneumococcal load in rabbit lungs when instilled concurrently with or 24 hours after Streptococcus pneumonia challenge and altered the progression of pre-existing pneumonia by preventing bacterial adherence (37). Prior incubation with LNnT also prevented adherence of S. pneumonia to chinchilla lungs (38). In addition to this, HMOs may play a systemic immunomodulatory role via direct mediation of cell-cell interactions in the immune system (41) when HMOs are absorbed intact into the circulation (26,28,42), and modulation of gut microbiota with indirect downstream effects on the immune system when largely indigestible HMOs reach the infant's colon (43). Duska-McEwen et al (40) recently reported that 2′FL and LNnT may decrease viral load in an in vitro model by enhancing innate immune responses to respiratory syncytial virus NM232 and influenza A virus H1N1 (NC99), 2 viruses associated with lower respiratory infections.

Additional findings on a lower likelihood of reported bronchitis and lower respiratory tract infection in the subset of cesarean-born infants who were fed test formula are notable, given this subgroup analysis included only slightly more than one-third of the infants in each feeding group. Well-documented aberrations in the early stages of gut colonization in cesarean-born infants (44–46) may make this group of infants particularly sensitive to nutritional interventions (eg, HMOs) that are aimed at supporting the development of a healthy gut microbiota and a healthy immune system in early infancy. It is also noteworthy that most of the morbidity-related differences between the test and control groups persisted through 12 months, which was beyond the first 6 months of age when HMOs were consumed. The potential immune-modulation of HMOs may be long-lasting; early exposure to HMOs may program the immune system (43) in a way that lowers the later risk of infections within the respiratory tract.

We did not observe an effect of HMOs on GI-related morbidities, although literature suggests such an effect may exist (11,34). Our results may relate to the low rate of GI-related morbidities in our study population, such as, gastroenteritis was reported in only 18% of infants between 0 and 12 months of age, compared with 38% of infants with reported bronchitis during the first year of life. Overall, our findings of lower respiratory tract–related morbidities in the test group, along with a recent study that found an association between breast milk 2′FL and a lower risk of allergic disease onset (including eczema) among infants delivered by cesarean section (47), suggest that HMOs may exert effects beyond the GI tract.

Back to Top | Article Outline

Strengths and Limitations

The current study was the first clinical trial evaluating infant formula supplemented with 2 HMOs (2′FL and LNnT), while previous studies (26,34,48,49) considered only a single HMO. The multicenter design and enrollment of infants by primary care pediatricians provided a representative sample of healthy term infants, supporting the generalizability of the results. In addition, infants were followed until 1 year of age, which allowed us to capture the potential sustained effects of HMOs consumed during the first 6 months of age. A limitation of the study is the absence of growth and secondary outcome data from a breast-fed reference group. In addition to this, GI tolerance data were based on parent report, which may be susceptible to under- or overeporting, although instructions from study staff on how to complete the diary, use of common terms for GI symptoms in the diary, and the double-blind study design reduced the risk that parents in the test group systematically differed from the control group in under- or overreporting these outcomes. The use of morbidity assessments based on parent-reported symptoms/illnesses and medication use, rather than a direct evaluation and diagnosis of each suspected illness by the study physician, is also a potential limitation. Although we were not able to implement the latter approach in this trial due to an increased burden on study participants and sites, any illness-related information reported by the parent was carefully reviewed and verified by study physicians at each clinic visit, and AEs of interest were well defined and identified a priori. Nonetheless, additional studies are needed to confirm whether HMO-supplemented infant formulas confer protection from illness. If replicated, the effects of HMOs on morbidity and medication use will carry substantial positive implications for the health of infants. In addition to this, while our findings are consistent with preclinical evidence on potential underlying mechanisms for the effects of HMOs, confirmation is needed in future trials that include evaluation of biomarkers to investigate biologic mechanisms. Future trials should also consider inclusion of outcomes related to stool volume and weight, which would provide insight on whether the addition of HMOs had an osmotic effect.

Back to Top | Article Outline


This randomized trial demonstrated that infant formula supplemented with 2′FL and LNnT is safe and well-tolerated. The HMO-supplemented formula supported normal, age-appropriate infant growth during the 4-month exclusive feeding period, after the introduction of complementary foods from 4 to 6 months, and following the switch to a standard follow-up formula without HMOs at 6 months up to 12 months of age. In addition, secondary outcome findings showed associations between formula supplemented with 2′FL and LNnT and lower rates of parent-reported morbidity (particularly bronchitis) and medication use. These secondary findings warrant confirmation in future studies.

Back to Top | Article Outline


The authors thank the families and caregivers who consented to their infants’ participation in this study, as well as the investigators and their study teams for their major contributions to this study. The authors also thank Isabelle Cristiani, Annemarie Beekman, and William Sauret for assistance with trial management, Jian Yan for critical review and comments on earlier drafts of the manuscript, and Nicole Cooper for editorial assistance funded by Nestlé Nutrition.

Back to Top | Article Outline


1. Zivkovic AM, German JB, Lebrilla CB, et al. Human milk glycobiome and its impact on the infant gastrointestinal microbiota. Proc Natl Acad Sci U S A 2011; 108 (suppl 1):4653–4658.
2. Coppa GV, Zampini L, Galeazzi T, et al. Prebiotics in human milk: a review. Dig Liver Dis 2006; 38 (suppl 2):S291–S294.
3. Coppa GV, Pierani P, Zampini L, et al. Oligosaccharides in human milk during different phases of lactation. Acta Paediatr Suppl 1999; 88:89–94.
4. Hamosh M. Bioactive factors in human milk. Pediatr Clin North Am 2001; 48:69–86.
5. Bao Y, Chen C, Newburg DS. Quantification of neutral human milk oligosaccharides by graphitic carbon high-performance liquid chromatography with tandem mass spectrometry. Anal Biochem 2013; 433:28–35.
6. Tao N, DePeters EJ, Freeman S, et al. Bovine milk glycome. J Dairy Sci 2008; 91:3768–3778.
7. Galeotti F, Coppa GV, Zampini L, et al. Capillary electrophoresis separation of human milk neutral and acidic oligosaccharides derivatized with 2-aminoacridone. Electrophoresis 2014; 35:811–818.
8. Galeotti F, Coppa GV, Zampini L, et al. On-line high-performance liquid chromatography-fluorescence detection-electrospray ionization-mass spectrometry profiling of human milk oligosaccharides derivatized with 2-aminoacridone. Anal Biochem 2012; 430:97–104.
9. Thurl S, Munzert M, Henker J, et al. Variation of human milk oligosaccharides in relation to milk groups and lactational periods. Br J Nutr 2010; 104:1261–1271.
10. Chaturvedi P, Warren CD, Altaye M, et al. Fucosylated human milk oligosaccharides vary between individuals and over the course of lactation. Glycobiology 2001; 11:365–372.
11. Morrow AL, Ruiz-Palacios GM, Altaye M, et al. Human milk oligosaccharides are associated with protection against diarrhea in breast-fed infants. J Pediatr 2004; 145:297–303.
12. Erney RM, Malone WT, Skelding MB, et al. Variability of human milk neutral oligosaccharides in a diverse population. J Pediatr Gastroenterol Nutr 2000; 30:181–192.
13. Coppa GV, Gabrielli O, Zampini L, et al. Oligosaccharides in 4 different milk groups, Bifidobacteria, and Ruminococcus obeum. J Pediatr Gastroenterol Nutr 2011; 53:80–87.
14. Nakhla T, Fu D, Zopf D, et al. Neutral oligosaccharide content of preterm human milk. Br J Nutr 1999; 82:361–367.
15. Asakuma S, Hatakeyama E, Urashima T, et al. Physiology of consumption of human milk oligosaccharides by infant gut-associated bifidobacteria. J Biol Chem 2011; 286:34583–34592.
16. Hong Q, Ruhaak LR, Totten SM, et al. Label-free absolute quantitation of oligosaccharides using multiple reaction monitoring. Anal Chem 2014; 86:2640–2647.
17. Smilowitz JT, O'Sullivan A, Barile D, et al. The human milk metabolome reveals diverse oligosaccharide profiles. J Nutr 2013; 143:1709–1718.
18. Sumiyoshi W, Urashima T, Nakamura T, et al. Determination of each neutral oligosaccharide in the milk of Japanese women during the course of lactation. Br J Nutr 2003; 89:61–69.
19. Leo F, Asakuma S, Fukuda K, et al. Determination of sialyl and neutral oligosaccharide levels in transition and mature milks of Samoan women, using anthranilic derivatization followed by reverse phase high performance liquid chromatography. Biosci Biotechnol Biochem 2010; 74:298–303.
20. Asakuma S, Urashima T, Akahori M, et al. Variation of major neutral oligosaccharides levels in human colostrum. Eur J Clin Nutr 2008; 62:488–494.
21. Lewis ZT, Totten SM, Smilowitz JT, et al. Maternal fucosyltransferase 2 status affects the gut bifidobacterial communities of breastfed infants. Microbiome 2015; 3:13.
22. Bode L. The functional biology of human milk oligosaccharides. Early Hum Dev 2015; 91:619–622.
23. Yu ZT, Chen C, Newburg DS. Utilization of major fucosylated and sialylated human milk oligosaccharides by isolated human gut microbes. Glycobiology 2013; 23:1281–1292.
24. Marcobal A, Barboza M, Sonnenburg ED, et al. Bacteroides in the infant gut consume milk oligosaccharides via mucus-utilization pathways. Cell Host Microbe 2011; 10:507–514.
25. He Y, Liu S, Kling DE, et al. The human milk oligosaccharide 2′-fucosyllactose modulates CD14 expression in human enterocytes, thereby attenuating LPS-induced inflammation. Gut 2016; 65:33–46.
26. Marriage BJ, Buck RH, Goehring KC, et al. Infants fed a lower calorie formula with 2′FL show growth and 2′FL uptake like breast-fed infants. J Pediatr Gastroenterol Nutr 2015; 61:649–658.
27. Jantscher-Krenn E, Bode L. Human milk oligosaccharides and their potential benefits for the breast-fed neonate. Minerva Pediatr 2012; 64:83–99.
28. Goehring KC, Kennedy AD, Prieto PA, et al. Direct evidence for the presence of human milk oligosaccharides in the circulation of breastfed infants. PLoS One 2014; 9:e101692.
29. Coulet M, Phothirath P, Allais L, et al. Pre-clinical safety evaluation of the synthetic human milk, nature-identical, oligosaccharide 2′-O-Fucosyllactose (2′FL). Regul Toxicol Pharmacol 2014; 68:59–69.
30. Coulet M, Phothirath P, Constable A, et al. Pre-clinical safety assessment of the synthetic human milk, nature-identical, oligosaccharide lacto-N-neotetraose (LNnT). Food Chem Toxicol 2013; 62:528–537.
31. American Academy of Pediatrics Task Force. Clinical Testing of Infant Formulas With Respect to Nutritional Suitability for Term Infants. US Food and Drug Administration Web site. Accessed July 8, 2016.
32. WHO Multicentre Growth Reference Study GroupWHO Child Growth Standards: Length/Height-for-age, Weight-for-age, Weight-for-length, Weight-for-height and Body Mass Index-for-age: Methods and Development. Geneva, Switzerland:World Health Organization; 2006.
33. Puccio G, Cajozzo C, Meli F, et al. Clinical evaluation of a new starter formula for infants containing live Bifidobacterium longum BL999 and prebiotics. Nutrition 2007; 23:1–8.
34. Newburg DS, Ruiz-Palacios GM, Altaye M, et al. Innate protection conferred by fucosylated oligosaccharides of human milk against diarrhea in breastfed infants. Glycobiology 2004; 14:253–263.
35. Donovan SM. Role of human milk components in gastrointestinal development: current knowledge and future needs. J Pediatr 2006; 149:S49–S61.
36. Weichert S, Jennewein S, Hüfner E, et al. Bioengineered 2′-fucosyllactose and 3-fucosyllactose inhibit the adhesion of Pseudomonas aeruginosa and enteric pathogens to human intestinal and respiratory cell lines. Nutr Res 2013; 33:831–838.
37. Idänpään-Heikkilä I, Simon PM, Zopf D, et al. Oligosaccharides interfere with the establishment and progression of experimental pneumococcal pneumonia. J Infect Dis 1997; 176:704–712.
38. Tong HH, McIver MA, Fisher LM, et al. Effect of lacto-N-neotetraose, asialoganglioside-GM1 and neuraminidase on adherence of otitis media-associated serotypes of Streptococcus pneumoniae to chinchilla tracheal epithelium. Microb Pathog 1999; 26:111–119.
39. Thomas R, Brooks T. Common oligosaccharide moieties inhibit the adherence of typical and atypical respiratory pathogens. J Med Microbiol 2004; 53:833–840.
40. Duska-McEwen G, Senft AP, Ruetschilling TL, et al. Human milk oligosaccharides enhance innate immunity to respiratory syncytial virus and influenza in vitro. Food Nutr Sci 2014; 5:1387–1398.
41. Bode L. Recent advances on structure, metabolism, and function of human milk oligosaccharides. J Nutr 2006; 136:2127–2130.
42. Ruhaak LR, Stroble C, Underwood MA, et al. Detection of milk oligosaccharides in plasma of infants. Anal Bioanal Chem 2014; 406:5775–5784.
43. Jost T, Lacroix C, Braegger C, et al. Impact of human milk bacteria and oligosaccharides on neonatal gut microbiota establishment and gut health. Nutr Rev 2015; 73:426–437.
44. Jakobsson HE, Abrahamsson TR, Jenmalm MC, et al. Decreased gut microbiota diversity, delayed Bacteroidetes colonisation and reduced Th1 responses in infants delivered by caesarean section. Gut 2014; 63:559–566.
45. Biasucci G, Rubini M, Riboni S, et al. Mode of delivery affects the bacterial community in the newborn gut. Early Hum Dev 2010; 86 (suppl 1):13–15.
46. Huurre A, Kalliomäki M, Rautava S, et al. Mode of delivery—effects on gut microbiota and humoral immunity. Neonatology 2008; 93:236–240.
47. Sprenger N, Odenwald H, Kukkonen AK, et al. FUT2-dependent breast milk oligosaccharides and allergy at 2 and 5 years of age in infants with high hereditary allergy risk. Eur J Nutr 2016; [Epub ahead of print].
48. Ukkonen P, Varis K, Jernfors M, et al. Treatment of acute otitis media with an antiadhesive oligosaccharide: a randomised, double-blind, placebo-controlled trial. Lancet 2000; 356:1398–1402.
49. Prieto PA. In vitro and clinical experiences with a human milk oligosaccharide, Lacto-N-neotetraose, and fructooligosaccharides. Foods Food Ingredients J Japan 2005; 210:1018–1030.

2′fucosyllactose; bronchitis; lacto-N-neotetraose; safety; tolerance

Supplemental Digital Content

Back to Top | Article Outline
© 2017 by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology,