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P0911 LACTOBACILLUS CASEI DN-114 001 INHIBITS THE INCREASE OF PARACELLULAR PERMEABILITY IN ENTEROPATHOGENIC ESCHERICHIA COLI-INFECTED T84 CELLS

Freitas, M.1; Nadege, P.2; Raphaelle, B.1; Rampal, P.2

Journal of Pediatric Gastroenterology and Nutrition: June 2004 - Volume 39 - Issue - p S404
ABSTRACTS: Poster Session Abstracts

1Nutrivaleur, Danone Vitapole, Palaiseau,2Laboratoire de Gastroenterologie, Université de Nice Sophia-Antipolis, Nice, France

Submitted by: miguel.freitas@danone.com

Introduction: Probiotics are living micro-organisms, that, when ingested in adequate amount exert health benefits to the host and contribute to the reinforcement of the intestinal epithelial barrier function.

The goal of this study was to determine if Lactobacillus casei DN-114 001 could abrogate the increase of paracellular permeability induced by Enteropathogenic Escherichia coli (EPEC).

Methods: We used the human colon T84 cell line infected with the wild type EPEC strain E2348/69. The paracellular permeability was observed by monitoring transepithelial electrical resistance (TER) variations and by observing Zonula Occludens-1 (ZO-1) distribution. Two infection procedures were used; coincubation (pathogenic and probiotic strains were simultaneous incubated with T84 cells) and postinfection (probiotic was added three hours after the beginning of the infection). We also investigated the effect of Lactobacillus casei on EPEC adherence.

Results: In this study, we demonstrated that Lactobacillus casei DN-114 001 inhibits in a dose-dependent-manner, EPEC-induced TER decrease and ZO-1 redistribution, whatever the infection procedure used, however, it does not inhibit pathogenic strain adhesion.

Conclusion: Altogether, this study shows that Lactobacillus casei DN-114 001 is able to inhibit EPEC-induced paracellular permeability increase. This protective effect seems independent on EPECs adhesion inhibition.

© 2004 Lippincott Williams & Wilkins, Inc.