Skip Navigation LinksHome > September 2014 - Volume 59 - Issue 3 > Tryptophan Requirement of the Enterally Fed Term Infant in t...
Journal of Pediatric Gastroenterology & Nutrition:
doi: 10.1097/MPG.0000000000000434
Original Articles: Hepatology and Nutrition

Tryptophan Requirement of the Enterally Fed Term Infant in the First Month of Life

Huang, Lisha*; Hogewind-Schoonenboom, Jacomine E.*; Zhu, Li; Kraaijenga, Juliette V.S.*; van Haren, Nicky P.C.§; Voortman, Gardi J.; Schierbeek, Henk*; Twisk, Jos W.R.||; Huang, Ying; Chen, Chao; van Goudoever, Johannes B.*

Collapse Box

Abstract

Objectives: Tryptophan not only is an amino acid essential to protein synthesis but also serves as a precursor in 2 important metabolic pathways: the serotonin and the kynurenine pathways. Tryptophan is related to sleeping patterns. The objective of the present study was to determine the tryptophan requirement of term infants using the indicator amino acid oxidation (IAAO) method with L-[1-13C]phenylalanine as the indicator.

Methods: Enterally fed infants were randomly assigned to tryptophan intakes ranging from 0.5 to 73 mg · kg−1 · day−1 as part of an elemental diet. After 1-day adaptation to the test diet, [13C]bicarbonate and L-[1-13C]phenylalanine tracers were given enterally. Breath samples were collected at baseline and during isotopic plateaus. The mean tryptophan requirement was determined by using the biphasic linear regression crossover analysis on the fraction of 13CO2 recovery from L-[1-13C]phenylalanine oxidation (F13CO2). Data are presented as mean ± standard deviation.

Results: A total of 30 term neonates (gestational age 39 ± 1 weeks) were studied at 9 ± 4 days. F13CO2 decreased until a tryptophan intake of 15 mg · kg−1 · day−1; additional increases in tryptophan intake did not affect F13CO2. Mean requirement was determined to be 15 mg · kg−1 · day−1.

Conclusions: The mean tryptophan requirement for elemental formula-fed term infants is 15 mg · kg−1 · day−1. This requirement is lower than the present recommended intake of 29 mg · kg−1 · day−1, which is based on the average intake of a breastfed infant.

© 2014 by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology,

Login

Article Tools

Share

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.

Connect With Us

 

 

Twitter

twitter.com/JPGNonline

 

Visit JPGN.org on your smartphone. Scan this code (QR reader app required) with your phone and be taken directly to the site.