Background: Tryptophan is not only an amino acid essential to protein synthesis, it also serves as a precursor in two important metabolic pathways: the serotonin and the kynurenine pathways. Tryptophan is related to sleeping patterns.
Objective: The objective was to determine the tryptophan requirement of term infants using the Indicator Amino Acid Oxidation (IAAO) method with L-[1-13C]phenylalanine as the indicator.
Methods: Enterally fed infants were randomly assigned to tryptophan intakes ranging from 0.5 to 73 mg/kg per day as part of an elemental diet. After 1 day adaptation to the test diet, [13C]bicarbonate and L-[1-13C]phenylalanine tracers were given enterally. Breath samples were collected at baseline and during isotopic plateaus. The mean tryptophan requirement was determined by using the biphasic linear regression crossover analysis on the fraction of 13CO2 recovery from L-[1-13C]phenylalanine oxidation (F13CO2). Data is presented as mean +/- SD.
Results: Thirty term neonates (gestational age 39 +/- 1 wk) were studied at 9 +/- 4 days. F13CO2 decreased until a tryptophan intake of 15 mg/kg per day; additional increases in tryptophan intake did not affect F13CO2. Mean requirement was determined to be 15 mg/kg per day.
Conclusions: The mean tryptophan requirement for elemental formula fed term infants is 15 mg/kg per day. This requirement is lower than the current recommended intake of 29 mg/kg per day, which is based on the average intake of a breastfed infant.
(C) 2014 by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology,