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Single-Nucleotide Polymorphisms of IL-10 and IL-28B as Predictors of the Response of IFN Therapy in HCV Genotype 4infected Children

Shaker, Olfat G.*; Nassar, Yasser H.*; Nour, Zeinab A.*; El Raziky, Mona

Journal of Pediatric Gastroenterology & Nutrition:
doi: 10.1097/MPG.0b013e31828febf0
Original Articles: Hepatology and Nutrition
Abstract

Background and Aims: Single-nucleotide polymorphisms (SNPs) in the IL-10 gene (−1082 [rs1800896], −819 [rs3021097], and −592 [rs1800872]) and the IL-28B gene (rs12979860) in adults were shown to be associated with hepatitis C virus (HCV) clearance. The present study aimed to investigate the possible association of SNPs of IL-10 and IL-28B in predicting the treatment response of HCV genotype 4 in pediatric patients.

Patients and Methods: A restriction fragment length polymorphism-polymerase chain reaction and real-time polymerase chain reaction techniques were used to genotype 34 pediatric patients with HCV genotype 4 for IL-10 and IL-28B SNPs, respectively. Patients received pegylated interferon-α/ribavirin for 48 weeks subdivided according to their response to treatment into responders and nonresponders; also, 20 healthy individuals served as controls.

Results: A significant difference (P < 0.005) was observed in SNP of IL-28B rs12979860 frequencies between responders and nonresponders. In responders, CC genotype had greater frequency than CT and TT genotypes (60%, 30%, 10%), respectively, with C allele in its homozygous (CC) genotype more likely to respond to treatment than in its homozygous (TT) genotypes. SNPs of IL-10 at −819 (rs3021097) showed significant differences in their genotype frequencies between responders and nonresponders to therapy, and TT genotype had greater frequency in responders than CT and CC (55%, 20%, 25%), respectively. Genotypes with T allele (CT/TT) showed higher rates of response than those with no T allele (CC).

Conclusions: SNPs of the IL-28B gene at (rs12979860) CC genotype as well as the IL-10 gene SNPs at −819 (rs3021097)TT genotype can be used for predicting response to treatment before patients are prescribed the expensive pegylated interferon-α/ribavirin therapy.

Author Information

*Department of Medical Biochemistry and Molecular Biology

Department of Pediatrics, Faculty of Medicine, Cairo University, Cairo 02, Egypt.

Address correspondence and reprint requests to Olfat G. Shaker, MD, Faculty of Medicine, Cairo University, Cairo, Egypt (e-mail: olfatshaker@yahoo.com).

Received 27 October, 2012

Accepted 1 March, 2013

This work was supported by grant from the Egyptian Science and Technology Development Fund (project number 1512 to O.S.) and fund from the Cairo University for the same PI.

The authors report no conflicts of interest.

© 2013 by European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology,