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Prevention of Rotavirus-induced Diarrhea by Preferential Secretion of IgA in Breast Milk via Maternal Administration of Lactobacillus gasseri SBT2055

Kadooka, Yukio*; Tominari, Kazue; Sakai, Fumihiko*; Yasui, Hisako

Journal of Pediatric Gastroenterology & Nutrition: July 2012 - Volume 55 - Issue 1 - p 66–71
doi: 10.1097/MPG.0b013e3182533a2b
Original Articles: Gastroenterology

Objectives: Rotavirus (RV)-induced diarrhea poses a major health problem, particularly to infants. An effective measure to prevent RV infection is to consume breast milk with higher levels of protective IgA. We therefore examined whether Lactobacillus gasseri SBT2055 (LG2055) could augment immumoglobulin (Ig) A levels and reduce the incidence of diarrhea in a mouse model of RV infection.

Methods: Female BALB/c mouse dams were fed a diet containing 0.1% heat-treated LG2055 or a control, beginning 4 weeks before mating with male mice and continuing until the experiment ended. One week after mating, female dams were immunized orally with simian RV SA-11. Five days after birth, mouse pups were infected orally with RV and the incidence of diarrhea was determined 4 days later. RV-specific and total IgA were quantified by an enzyme-linked immunosorbent assay.

Results: LG2055-fed dams immunized with RV (LG2055/RV) secreted breast milk that significantly lowered the incidence of RV-induced diarrhea in their pups as compared with dams immunized with RV alone (C/RV). The LG2055/RV dams also produced a significantly greater amount of RV-specific IgA in breast milk obtained from the pups’ stomach, but not in feces or Peyer's patch cell cultures. In addition, LG2055 stimulated total IgA production in splenocyte cultures from Toll-like receptor (TLR)-4-knockout mice, but not those from TLR-2-knockouts.

Conclusions: LG2055-fed dams reduced RV infection in their pups and elevated RV-specific IgA levels in breast milk of stomach origin, the possible mechanism of which may be TLR-2 stimulation by LG2055.

*Milk Science Research Institute, Megmilk Snow Brand Co Ltd, Kawagoe, Saitama, Japan

Science of Functional Foods, Graduate School of Agriculture, Shinshu University, Kamiina, Nagano, Japan.

Address correspondence and reprint requests to Yukio Kadooka, Milk Science Research Institute, Megmilk Snow Brand Co Ltd, 1-1-2 Minamidai, Kawagoe, Saitama 350-1165, Japan (e-mail: y-kadooka@meg-snow.com).

Received 1 September, 2011

Accepted 4 December, 2011

The authors report no conflicts of interest.

Copyright 2012 by ESPGHAN and NASPGHAN