Horizontal Distribution of the Fecal Microbiota in Adolescents With Inflammatory Bowel Disease

Gosiewski, Tomasz*; Strus, Magdalena*; Fyderek, Krzysztof; Kowalska-Duplaga, Kinga; Wedrychowicz, Andrzej; Jedynak-Wasowicz, Urszula; Sladek, Malgorzata; Pieczarkowski, Stanislaw; Adamski, Pawel§; Heczko, Piotr B.*

Journal of Pediatric Gastroenterology & Nutrition:
doi: 10.1097/MPG.0b013e31822d53e5
Original Articles: Gastroenterology
Abstract

Background and Aims: The commensal microbiota of the gastrointestinal tract plays an important role in the pathogenesis of inflammatory bowel disease. We examined the horizontal structure of the fecal microbiota in the colon in adolescents with Crohn disease or ulcerative colitis and a control group.

Patients and Methods: Fecal samples were collected in 3 fractions from patients with Crohn disease (n = 22), ulcerative colitis (n = 12), and controls (n = 24) during preparation for colonoscopy. Additionally, biopsies from colon tissue were taken. Samples were examined using a culture technique and a fluorescent in situ hybridization method. The mucin degradation assay was carried out.

Results: Quantitative composition of the microbiota was different in the consecutive 3 fecal fractions and in the colon tissue of the study groups, but in patients from the control group, the composition of microbiota in the consecutive fractions was similar. Statistical analyses showed that the total distribution of the studied bacterial taxons in the contents in all 3 fecal fractions and in the colon tissue in the given disease group, and in the control group was characteristic for the studied patient group. Differences in species distribution among the cohorts studied were highly significant (P < 0.0001). Moreover, it was shown that in the fecal fraction I and in the colon tissue samples, there is no significant difference for any of the analyzed bacterial groups, using the culture methods or fluorescent in situ hybridization, but significant results were demonstrated in the II and III fractions for specific bacterial groups. The bacterial flora attached to the mucus layer in the UC group had significantly more degraded mucus in comparison with the control group (P = 0.045).

Conclusions: Distribution of the microbiota in the colon is layered, which can be called horizontal distribution of the fecal flora. Only in the ulcerative colitis group, the bacterial flora attached to the mucous layer exerts action on the mucin.

Author Information

*Department of Microbiology, Jagiellonian University Medical College

Department of Pediatrics, Gastroenterology and Nutrition

Department of Pediatrics, Polish-American Children's Hospital, Jagiellonian University Medical College

§Institute of Nature Conservation, Polish Academy of Sciences, Cracow, Poland.

Address correspondence and reprint requests to Dr Tomasz Gosiewski, PhD, Chair of Microbiology, Jagiellonian University Medical College, 18 Czysta St, 31-121 Cracow, Poland (e-mail: tomasz.gosiewski@uj.edu.pl).

Received 15 March, 2011

Accepted 7 July, 2011

The present study was supported by Polish Ministry of Science and Higher Education within the framework of the 3-year project grant 3 P05E 091 25 and the 2-year doctoral grant N406 029/31/0892.

The authors report no conflicts of interest.

Copyright 2012 by ESPGHAN and NASPGHAN