Objectives: Constipation can adversely affect children's health, with disorders of host immunity and enhanced oxidative stress. As nondigestible carbohydrates, prebiotics can affect the host with constipation; however, whether the prebiotics have effects on the content of intestinal secretory immunoglobulin A (sIgA) and the contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in constipation has not been fully clarified.
Methods: In the present study, constipation was induced in female Sprague-Dawley rats by diphenoxylate, and the prebiotics dissolved in milk were used as an intervention. The indicators of intestinal peristalsis, including the time of passing black stool initially, the grains of black stool in 24 hours, and the advance rate of ponceau, were measured. The content of intestinal sIgA was detected by enzyme-linked immunosorbent assay. The contents of SOD and MDA in serum and intestinal tissue were analyzed by their detection kits.
Results: The changes in intestinal peristalsis show obvious constipation. The content of intestinal sIgA decreases, the content of SOD decreases, but the content of MDA increases in constipated rats. Prebiotics can attenuate the constipation-caused abnormal indicators significantly.
Conclusions: Prebiotics can attenuate decreased intestinal immunity and enhanced oxidative stress, in addition to reduced intestinal peristalsis and of the constipated rats.
*Hebei Key Laboratory of Laboratory Animals, Department of Molecular Biology
†Department of Biochemistry, Hebei Medical University, Shijiazhuang, Hebei Province, PR China.
Address correspondence and reprint requests to Prof Jinsheng Qi, Department of Biochemistry, Hebei Medical University, No. 361 East Zhongshan Rd, Shijiazhuang 050017, Hebei Province, PR China (e-mail: email@example.com).
Received 18 December, 2010
Accepted 11 April, 2011
Yanning Li and Yanhong Zong contributed equally to the work.
This study was funded by the Hebei Natural Science Foundation (C2009001092).
The authors report no conflicts of interest.