Skip Navigation LinksHome > June 2010 - Volume 50 - Issue 6 > Bilirubin, a Physiological Antioxidant, Can Improve Cryopres...
Journal of Pediatric Gastroenterology & Nutrition:
doi: 10.1097/MPG.0b013e3181cd26e5
Short Communication

Bilirubin, a Physiological Antioxidant, Can Improve Cryopreservation of Human Hepatocytes

Galbiati, G*; Muraca, M*; Mitry, RR; Hughes, RD; Lehec, SC; Puppi, J; Sagias, FG; Caruso, M*; Mieli-Vergani, G; Dhawan, A

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The availability of cryopreserved hepatocytes is required for a more widespread use of hepatocyte transplantation, but human hepatocytes are easily damaged during freezing-thawing. Here, preincubation with unconjugated bilirubin, a physiological antioxidant, resulted in increased viability and function of hepatocytes (as determined by trypan blue exclusion, mitochondrial succinate dehydrogenases activity, urea synthesis, and cytochrome P450 1A/2) compared with cells incubated without the pigment. These findings suggest that unconjugated bilirubin may be used as cryoprotectant in clinical hepatocyte transplantation.

© 2010 Lippincott Williams & Wilkins, Inc.


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