Skip Navigation LinksHome > June 2010 - Volume 50 - Issue 6 > Bilirubin, a Physiological Antioxidant, Can Improve Cryopres...
Journal of Pediatric Gastroenterology & Nutrition:
doi: 10.1097/MPG.0b013e3181cd26e5
Short Communication

Bilirubin, a Physiological Antioxidant, Can Improve Cryopreservation of Human Hepatocytes

Galbiati, G*; Muraca, M*; Mitry, RR; Hughes, RD; Lehec, SC; Puppi, J; Sagias, FG; Caruso, M*; Mieli-Vergani, G; Dhawan, A

Collapse Box

Abstract

The availability of cryopreserved hepatocytes is required for a more widespread use of hepatocyte transplantation, but human hepatocytes are easily damaged during freezing-thawing. Here, preincubation with unconjugated bilirubin, a physiological antioxidant, resulted in increased viability and function of hepatocytes (as determined by trypan blue exclusion, mitochondrial succinate dehydrogenases activity, urea synthesis, and cytochrome P450 1A/2) compared with cells incubated without the pigment. These findings suggest that unconjugated bilirubin may be used as cryoprotectant in clinical hepatocyte transplantation.

© 2010 Lippincott Williams & Wilkins, Inc.

Login

Article Tools

Share

Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.

Connect With Us

 

 

Twitter

twitter.com/JPGNonline