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Human Faecal Microbiota Develops the Ability to Degrade Type 3 Resistant Starch During Weaning

Scheiwiller, Judith*; Arrigoni, Eva*; Brouns, Fred†,‡; Amadò, Renato*

Journal of Pediatric Gastroenterology & Nutrition: November 2006 - Volume 43 - Issue 5 - p 584–591
doi: 10.1097/01.mpg.0000237937.05050.0d
Original Articles: Gastroenterology

Background: Colonisation of the human colon starts immediately after birth. Bacterial composition is substantially influenced by the type of feeding. During weaning, microbiota diversifies considerably to finally approach the composition of that of adults. The aim of this study was to investigate the ability of colonic microbiota obtained from different age groups to ferment resistant starch (RS).

Methods: Faecal samples of breast-fed and formula-fed infants, infants at weaning, adults and elderly subjects were used as inocula. Fermentation experiments were performed by applying a standardised in vitro batch method. Fermentability was established by measuring both metabolite production and substrate degradation. An RS type 3 (RS-3) was used as substrate; its behaviour was compared with that of lactulose (positive control), whereas inoculum without substrate was used as negative control.

Results: Overall fermentation patterns clearly showed that the human microbiota of all age groups is able to degrade lactulose. In contrast, RS-3 was found resistant to the attack by microbiota of both breast-fed and formula-fed infants. Bacteria collected from infants at weaning were able to degrade RS-3 completely, but slower compared with adults. With increasing age, RS-3 fermentation was observed to be slightly retarded again.

Conclusions: Human faecal microbiota of all age groups is able to ferment lactulose in vitro quickly and completely. The ability to degrade RS-3, however, is only established during weaning. Whether fermentation-related production of short-chain fatty acids from RS-3 and concurrent modifications of the microbiota can result in potential health benefits to the host at this stage of life needs to be elucidated.

*Swiss Federal Institute of Technology (ETH), Institute of Food Science and Nutrition, Zurich, Switzerland

Cargill R and D Centre, Vilvoorde, Belgium

Maastricht University, Nutrition and Toxicology Research Institute, Maastricht, The Netherlands

Received 27 December, 2005

Accepted 23 July, 2006

Address correspondence and reprint requests to Eva Arrigoni, Swiss Federal Institute of Technology (ETH), Institute of Food Science and Nutrition, Laboratory of Food Chemistry and Technology (ETH), Zurich, Switzerland (e-mail: eva.arrigoni@ilw.agrl.ethz.ch).

Supported by a research grant from Cargill-Cerestar.

Dr Fred Brouns is active at the Cargill R and D Centre, Vilvoorde, Belgium. Cargill produces and markets Actistar.

© 2006 Lippincott Williams & Wilkins, Inc.