Skip Navigation LinksHome > March 2014 - Volume 56 - Issue 3 > Paraoxonase 1 and Its Relationship With Pesticide Biomarkers...
Journal of Occupational & Environmental Medicine:
doi: 10.1097/01.jom.0000438381.25597.88
Original Articles

Paraoxonase 1 and Its Relationship With Pesticide Biomarkers in Indigenous Mexican Farmworkers

Bernal-Hernández, Yael Yvette PhD; Medina-Díaz, Irma Martha PhD; Barrón-Vivanco, Briscia Socorro PhD; Robledo-Marenco, María de Lourdes PhD; Girón-Pérez, Manuel Iván PhD; Pérez-Herrera, Norma Elena PhD; Quintanilla-Vega, Betzabet PhD; Cerda-Flores, Ricardo PhD; Rojas-García, Aurora Elizabeth PhD

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Abstract

Objective: Biomarkers of pesticide toxicity and paraoxonase 1 (PON1) phenotype and genotypes were evaluated in indigenous Mexican farmworkers exposed mainly to organophosphate (OP) pesticides.

Methods: Acetylcholinesterase, butyrylcholinesterase, and PON1 activities—arylesterase and CMPAase activities—were evaluated spectrophotometrically. PON1 55 and 192 polymorphisms were determined by real-time polymerase chain reaction. Hematological parameters were evaluated using a cytometer.

Results: Butyrylcholinesterase and arylesterase activities were lower in farmworkers, who also showed lower levels of leukocytes but higher percentages of lymphocytes when compared with the nonexposed group. Our results showed a high frequency of OP, high hydrolysis-related PON1 alleles (LL/QR and LL/RR) in the study population. An association was observed between CMPAase activity and PON1Q192R polymorphism.

Conclusions: Our results suggest that pesticide exposure modifies biochemical and hematological biomarkers in the study population, and that the phenotype of PON1 (CMPAase) is a sensible susceptibility biomarker of OP pesticide toxicity.

Copyright © 2014 by the American College of Occupational and Environmental Medicine

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