Objective: The work aimed to study the potential correlation between the high-level benzene exposure and its urinary metabolites S-phenylmercapturic acid (SPMA) and trans, trans-muconic acid (t,t-MA) in Chinese shoe-making workers.
Methods: Individual benzene-exposed levels were determined by gas chromatography analysis, urinary t,t-MA, and urinary SPMA were determined by high performance liquid chromatography-an ultraviolet detector and liquid chromatography/electrospray tandem mass spectrometry method, respectively.
Results: The concentration of benzene ranged from 2.57 to 146.11 mg/m3. And the correlation between benzene and t,t-MA was significantly higher than that of SPMA at the postshift, for example, the correlation coefficient was 0.905 and 0.537 for t,t-MA and SPMA, respectively. Moreover, The relative internal exposure index of t,t-MA (0.28 mg/g Cr: mg/m3) was more similar to the data supplied by American Conference of Governmental Industrial Hygienists compared to the index of SPMA (0.025 mg/g Cr:mg/m3).
Conclusions: t,t-MA appeared to be a more specific biomarker than SPMA at high-level benzene exposure.
From the Department of Preventive Medicine (Drs Zhang, Mei, and Song and Mr Ye), Medical College, Wuhan University of Science and Technology, Wuhan, China; and Ministry of Education Key Laboratory of Environment and Health (Dr Chen), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Address correspondence to: Shi-zhen Song, PhD, Department of Preventive Medicine, Medical College, Wuhan University of Science and Technology, Wuhan 430065, China; (firstname.lastname@example.org).