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Identification of Systemic Markers from A Pulmonary Carbon Nanotube Exposure

Erdely, Aaron PhD; Liston, Angie BS; Salmen-Muniz, Rebecca AAS; Hulderman, Tracy BS, MT; Young, Shih-Houng PhD; Zeidler-Erdely, Patti C. PhD; Castranova, Vincent PhD; Simeonova, Petia P. MD, PhD

Journal of Occupational and Environmental Medicine: June 2011 - Volume 53 - Issue - p S80–S86
doi: 10.1097/JOM.0b013e31821ad724
Epidemiologic Research: Original Article

Objective: Interest exists for early monitoring of worker exposure to engineered nanomaterials. Here, we highlight quantitative systemic markers of early effects after carbon nanotube (CNT) exposure.

Methods: Mice were exposed by pharyngeal aspiration to 40-μg CNT and harvested 24 hours, 7 days, and 28 days postexposure for measurements of whole blood, lung and extrapulmonary tissue gene expression, blood and bronchoalveolar lavage (BAL) differentials, and serum protein profiling.

Results: Early effects included increased inflammatory blood gene expression and serum cytokines followed by an acute phase response (eg, CRP, SAA-1, SAP). Beyond 24 hours, there was a consistent increase in blood and BAL eosinophils. At 28 day, serum acute phase proteins with immune function including complement C3, apolipoproteins A-I and A-II, and α2-macroglobulin were increased.

Conclusions: Carbon nanotube exposure resulted in measurable systemic markers but lacked specificity to distinguish from other pulmonary exposures.

From the Toxicology and Molecular Biology Branch (Dr Erdely, Dr Simeonova, Ms Liston, Ms Salmen-Muniz, Ms Hulderman), Pathology and Physiology Research Branch (Dr Young, Dr Zeidler-Erdely, Dr Castranova), and Laboratory of Occupational Cardiovascular Toxicology (Dr Erdely, Dr Castranova, Ms Salmen-Muniz, Ms Hulderman), Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WVa.

Deceased.

Address correspondence to: Aaron Erdely, PhD, NIOSH/HELD/TMBB, 1095 Willowdale Rd, MS-3014, Morgantown, WV 26505 (efi4@cdc.gov).

The findings and conclusions in this report are those of the author(s) and do not necessarily represent the views of the National Institute for Occupational Safety and Health.

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Copyright © 2011 by the American College of Occupational and Environmental Medicine