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Influence of Some Detoxification Enzyme Polymorphisms on Cytogenetic Biomarkers Between Individuals Exposed to Very Low Doses of 1,3-Butadiene

Bukvic, Nenad MD, PhD; Lovreglio, Piero MD, PhD; Fanelli, Margherita PhD; Susca, Francesco C. PhD; Ballini, Andrea BSc; Lastella, Patrizia MD; Foà, Vito MD, PhD; Fustinoni, Silvia MD, PhD; Soleo, Leonardo MD, PhD; Guanti, Ginevra MD, PhD

Journal of Occupational & Environmental Medicine:
doi: 10.1097/JOM.0b013e3181a88d7f
Original Articles
Abstract

Objective: To evaluate the variation of some biomarkers related to the level of enzymatic activity dependent on the different polymorphisms.

Methods: We studied 27 butadiene-exposed workers and 37 controls using different biomarkers of the genotoxic effect. The genotypes were determined using polymerase chain reaction and restriction fragment length polymorphism-polymerase chain reaction techniques; the subjects were assigned to a specific group based on the microsomal epoxide hydrolase (mEH) activity predicted by their genotype (low, intermediate, high).

Results: The studied biomarkers were not able to discriminate between exposed and control individuals, but sister chromatid exchange (SCE) and high frequency cells were influenced by smoking habits. Smokers having fast microsomal epoxide hydrolase activity showed higher SCE frequency (7.61) respect to those presenting intermediate (5.86) or slow (6.65) enzymatic activity.

Conclusions: On the basis of these results, can we suppose the existence of an “intermediate genotype” advantage (at least for induction of SCE)?

Author Information

From the Department of Internal and Public Medicine (Dr Bukvic, Dr Susca, Ms Ballini, Dr Lastella, Dr Guanti), Section of Medical Genetics, University of Bari, Bari; Department of Biomedical Science (Dr Bukvic), Section of Medical Genetics, University of Foggia, Foggia; Department of Clinical Pathology (Dr Bukvic), Section of Cytogenetics and Molecular Biology, University Hospital, Foggia; Department of Internal and Public Medicine (Dr Lovreglio, Dr Soleo), Section of Occupational Medicine “E.C. Vigliani,” University of Bari, Bari; Department of Internal and Public Medicine (Dr Fanelli), Section of Diagnostic Imaging, University of Bari, Bari; Department of Occupational Medicine (Dr Foà, Dr Fustinoni), University of Milan, Milan, Italy.

Address correspondence to: Nenad Bukvic, MD, PhD, Section of Cytogenetics and Molecular Biology, University Hospital, OORR Foggia, 71100 Foggia, Italy; E-mail: nenadbukvic@virgilio.it.

©2009The American College of Occupational and Environmental Medicine