Skip Navigation LinksHome > July 2007 - Volume 49 - Issue 7 > Screening of Serum Biomarkers and Establishment of a Decisio...
Journal of Occupational & Environmental Medicine:
doi: 10.1097/JOM.0b013e318058aa16
Original Articles

Screening of Serum Biomarkers and Establishment of a Decision Tree in Silica-Exposed Populations by Surface-Enhanced Laser Desorption Ionization Time-of-Fly Mass Spectrometry

Wang, Shi-Xin PhD; Zhao, Xue-Feng MMS; Wei, Mao-Ti MMS; Wu, You MMS; Zeng, Jia-Wei MMS; Liang, Xian-Cai BM; Yi, Jian-Hua MMS; He, Bing MMS; Tu, Zi-Guang MMS

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Abstract

Objective: To screen for serum biomarkers of silicosis and to study their roles using surface-enhanced laser desorption ionization time-of-fly mass spectrometry (SELDI-TOF-MS) techniques.

Methods: The serum protein/peptide profile on the CM10 proteinchip was acquired using SELDI-TOF-MS from workers classified by the Chinese national diagnostic standard for pneumoconiosis. Discriminant analysis was performed to establish a decision tree using protein/peptide peaks.

Results: Protein/peptide peaks changed much more in silica-exposed populations than they did in the control. Discriminant analysis using Wilks' lambda method could give high sensitivity and specificity in distinguishing the silica-exposed population from the control; the decision tree could give 100% sensitivity and 95% specificity in distinguishing the silicosis phase among the control group.

Conclusions: Special proteins/peptides may change in silica-exposed workers and these changes may be used to distinguish silica-exposed populations from the control with the proper discriminant analytical method.

©2007The American College of Occupational and Environmental Medicine

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