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Low Response in White Blood Cell DNA Adducts Among Workers in a Highly Polluted Cokery Environment

Kuljukka, Terhi MSc; Savela, Kirsti PhD; Vaaranrinta, Raija; Mutanen, Pertti MSc; Veidebaum, Toomas PhD; Sorsa, Marja PhD; Peltonen, Kimmo PhD

Journal of Occupational & Environmental Medicine: June 1998 - Volume 40 - Issue 6 - pp 529-537
Original Articles

Coke oven workers are often heavily exposed to polynuclear aromatic hydrocarbons (PAHs); this exposure has been associated with higher cancer rates among these workers. We assessed the exposure of cokery workers in an oil shale processing plant. Personal hygienic monitoring, measurement of urinary 1-hydroxypyrene (1-OHP), and analysis of PAH-DNA adducts in white blood cells (WBCs) were performed. The 32P-postlabeling method was used for adduct measurement. The mean adduct value, 1.6 adducts per 108 nucleotides, did not differ significantly from the control value(P = 0.098). Smokers had significantly higher adduct levels than non-smoking workers (P = 0.002). 1-OHP levels measured in post-shift samples correlated with DNA adducts found in white blood cells (WBCs). We conclude that hygienic monitoring and measurement of urinary metabolites are essential background exposure data when the biologically effective dose of chemical carcinogens is assessed.

From the Finnish Institute of Occupational Health, Helsinki, Finland (Ms Kuljukka, Dr Savela, Ms Vaaranrinta, Mr Mutanen, Dr Sorsa, and Dr Peltonen); the Chemistry Laboratory (Ms Kuljukka, Dr Savela, Ms Vaaranrinta, Dr Peltonen) and the Department of Epidemiology and Biostatistics (Mr Mutanen), the Finnish Institute of Occupational Health, Helsinki, Finland; and the Institute of Experimental and Clinical Medicine, Tallinn, Estonia (Dr Veidebaum).

Address correspondence to: Kimmo Peltonen, PhD, Finnish Institute of Occupational Health, Topeliuksenkatu 41 a A, SF-00250 Helsinki, Finland.

© Williams & Wilkins 1998. All Rights Reserved.