Journal of Neurologic Physical Therapy:
Motor Skill Learning Is Associated With Diffusion Characteristics of White Matter in Individuals With Chronic Stroke
Borich, Michael R. DPT, PhD; Brown, Katlyn E. MSc; Boyd, Lara A. PT, PhD
Department of Physical Therapy (M.R.B., L.A.B.), Graduate Program in Rehabilitation Sciences (K.E.B., L.A.B.), and Brain Research Centre (L.A.B.), University of British Columbia, Vancouver, British Columbia, Canada.
Correspondence: Lara A. Boyd, PT, PhD, Neurobiology of Motor Learning, University of British Columbia, 212–2177 Wesbrook Mall, Vancouver, BC V6T 2B5, Canada (firstname.lastname@example.org).
Funding received from NIH-NS051714 and CIHR MOP-106651.
This article contains work in part published in another form.
Supplemental digital content is available for this article. Direct URL citation appears in the printed text and is provided in the HTML and PDF versions of this article on the journal's Web site (www.jnpt.org).
The authors declare no conflicts of interest.
Background and Purpose: Imaging advances allow investigation of white matter after stroke; a growing body of literature has shown links between diffusion-based measures of white matter microstructure and motor function. However, the relationship between these measures and motor skill learning has not been considered in individuals with stroke. The aim of this study was to investigate the relationships between posttraining white matter microstructural status, as indexed by diffusion tensor imaging within the ipsilesional posterior limb of the internal capsule (PLIC), and learning of a novel motor task in individuals with chronic stroke.
Methods: A total of 13 participants with chronic stroke and 9 healthy controls practiced a visuomotor pursuit task across 5 sessions. Change in motor behavior associated with learning was indexed by comparing baseline performance with a delayed retention test. Fractional anisotropy (FA) indexed at the retention test was the primary diffusion tensor imaging-derived outcome measure.
Results: In individuals with chronic stroke, we discovered an association between posttraining ipsilesional PLIC FA and the magnitude of change associated with motor learning; hierarchical multiple linear regression analyses revealed that the combination of age, time poststroke, and ipsilesional PLIC FA posttraining was associated with motor learning-related change (R2 = 0.649; P = 0.02). Baseline motor performance was not related to posttraining ipsilesional PLIC FA.
Discussion and Conclusions: Diffusion characteristics of posttraining ipsilesional PLIC were linked to the magnitude of change in skilled motor behavior. These results imply that the microstructural properties of regional white matter indexed by diffusion behavior may be an important factor to consider when determining potential response to rehabilitation in persons with stroke.
Video Abstract available: (see Video, Supplemental Digital Content 1, http://links.lww.com/JNPT/A59) for more insights from the authors.
Past work robustly demonstrates that the learning of new motor skills is not abolished by stroke.1–3 However, the magnitude of motor learning-related change associated with a fixed amount of practice is highly variable across individuals with stroke, making it difficult to predict response to treatment and potential for recovery. In part, the degree of change that may occur in response to skilled motor practice depends on stroke severity. Boyd et al3 demonstrated lower slopes of change in individuals with moderate to severe stroke than those with more mild damage. However, this work examined motor learning when individuals employed their less-affected, nonhemiparetic arms and did not examine the relationship between residual white matter status and learning-related change.
Consideration of how residual brain tissue microstructure impacts motor learning-related change is key in advancing the understanding of which individuals with stroke may benefit from certain forms of rehabilitation aimed at restoration of motor function. A large body of work highlights the relationship between motor learning and changes in gray matter; developments in imaging technology now allow us to examine the relationship between white matter and learning. Diffusion tensor imaging (DTI) can provide a noninvasive characterization of white matter tissue status in both healthy individuals and individuals after stroke.4 Specifically, DTI-derived metrics, for example, fractional anisotropy (FA), can be used to determine the magnitude and direction of water diffusion, which are influenced by the microstructural properties of brain tissue.5 In particular, FA is the most commonly reported DTI-derived measure of white matter integrity and has been associated with function in neurologically intact individuals and persons with neurological injury.6–8
Past work examined white matter integrity in relation to training across different modalities. More specifically, training to improve knowledge of grammar rules, spelling, memory, or speech therapy in individuals with aphasia has all been linked to higher integrity (ie, FA) of white matter in the tracts corresponding with cortical areas supporting these behaviors.9–12 However, limited work has linked motor training with change in diffusion behavior (ie, increased FA) in regional white matter in healthy individuals. Past work has not considered whether DTI is sensitive to change in white matter microstructure associated with experience-dependent neuroplasticity in individuals with stroke or in older healthy adults. The few studies using DTI that show increased FA after training have employed very large doses of practice and been conducted in young healthy individuals. For example, after 6 weeks of practicing juggling, healthy individuals demonstrate increased FA values in the right posterior intraparietal sulcus.13 Interestingly, these increases in FA values were not related to performance improvements, suggesting that the training time, rather than performance outcome, is a key component to influencing white matter plasticity.13
A growing body of literature has examined white matter integrity using single time-point diffusion scans in stroke populations. Hemispheric asymmetry of FA values within the posterior limb of the internal capsule (PLIC) and the corticospinal tract have been shown to predict change in the Fugl-Meyer score following increased use of the hemiparetic arm to move a series of wooden blocks.14 Furthermore, the degree of impairment in function of the upper limb is closely linked to FA values in the PLIC, even more so than the blood oxygen level–dependent signal in motor regions during training.15 The contralesional and ipsilesional corticospinal tracts have also been linked to motor function on measures such as grip strength, hand dexterity, pegboard, and index finger tapping.16,17 Similar trends have been shown with a varying array of functional measures and the corresponding white matter tracts.18,19 Short-term practice effects also appear to be related to baseline FA values in the PLIC bilaterally.20 However, it remains unclear whether long-term practice effects are also mediated by white matter status in individuals with chronic stroke.
The purpose of this study was to evaluate how posttraining white matter status is linked to the magnitude of motor skill learning in individuals with chronic stroke. We hypothesized that ipsilesional white matter microstructural status at retention testing would be linked to motor learning-related change such that higher ipsilesional FA values would be associated with larger change in motor performance after training. Evaluation of these relationships is of interest for both neuroscientists and clinicians alike, and should inform our collective understanding of how white matter tissue status within the stroke-affected brain may affect the capacity for motor learning. Furthermore, a refined understanding of the relationships between microstructural status in the stroke-damaged brain and potential for motor learning-related change may enable clinicians to better predict response to, and evaluation of, rehabilitation interventions.
Thirteen well-recovered individuals with a single, clinically diagnosed chronic (>6 months poststroke)21 ischemic stroke affecting the middle cerebral artery distribution, mean age of 63.8 (SD = 1.8) years, and 9 healthy control participants, mean age of 66.0 (SD = 8.0) years, were recruited from the community (Table 1). The 2 groups had similar distributions of age, sex, and handedness. Informed consent was obtained from each participant in accordance with the Declaration of Helsinki. The University of British Columbia research ethics boards approved all aspects of the study protocol.
Individuals with a single middle cerebral artery ischemic stroke occurring more than 6 months before participation were included in this study. Individuals were excluded from participation if they had any other known neurological impairments, scored below the 25th percentile on the Mini-Mental Status Exam,22 scored below 15 on the Fugl-Meyer Upper Extremity Motor Scale,23 or had any contraindications to magnetic resonance imaging (MRI). In addition, participants required sufficient strength and motor control to grasp and manipulate a joystick to complete the skilled movements associated with our training task. Controls were excluded if they had known neurological impairments or contraindications to MRI.
Each participant completed 7 experimental sessions. The first session indexed baseline motor performance. Next, 5 practice sessions were completed on separate days, followed by a delayed retention test, which was performed on day 7. The data reported here were a part of a larger study that included multiple functional (f)MRI sessions. DTI data for this research were collected as a part of a single structural scanning battery that took place on day 7.
Visuomotor Pursuit Task
Participants sat in front of a computer monitor that displayed a white circle indicating the target and a red dot representing movement by the participant. Participants practiced tracking a moving target continuously using a joystick controlled by the hemiparetic arm (stroke group); healthy controls were matched for arm use (ie, right vs left) (Table 1). The participants' forearms were fixed into foam-lined trays throughout the task, which necessitated movements into radial and ulnar deviation to control the joystick. On each training day, 5 tracking task blocks, composed of 10 tracking trials (20s each), were completed. Although the target and movement were visible, visual feedforward and feedback information regarding the upcoming movement pattern or previous tracking performance was not available (ie, no pattern or trace was displayed on the computer screen). Each day participants were instructed to track the target as accurately as possible by controlling the position of the cursor with the joystick. At the completion of practice sessions, each participant completed 83.3 minutes of tracking training in total. The retention test consisted of a single block of the task (ten 20s tracking trials) completed in the functional (f)MRI scanner. This test was performed to assess improvement that had occurred as a result of previous training (as opposed to improvement within a single session). The time elapsed between each training session and between the final training session and retention test varied between 2 and 5 days.
Skill performance was quantified by the root mean square error (RMSE) between target and response over time of each trial individually. The RMSE was averaged across the first block of 10 (20s) trials for day 1 and day 7 assessments. A change score was calculated (day 7 − day 1) separately to assess improvement in tracking error associated with practice. Larger negative change scores represented greater reductions in visuomotor pursuit RMSE, indicative of training-associated improvements in tracking skill.
MRI Data Acquisition
MR acquisition was conducted at the University of British Columbia MRI Research Centre on a Philips Achieva 3.0T whole body MRI scanner (Phillips Healthcare, Andover, MD) using an 8-channel sensitivity encoding head coil (Sensitivity encoding factor = 2.4) and parallel imaging. A high-resolution anatomical scan (Repetitions Time [TR] = 12.4 ms; Echo Time [TE] = 5.4 ms; flip angle θ = 8°; Field of View [FOV] = 256 mm; 170 slices, 1 mm thickness) was collected. A diffusion-weighted scan was conducted with a single-shot echoplanar imaging sequence (TR = 7465 ms; TE = 75 ms; FOV = 212 × 212 mm; 60 slices, 2.2 mm slice thickness; voxel dimension = 2.2 mm3). Diffusion weighting was applied across 15 independent noncollinear orientations (b = 1000 s/mm2) along with a nonweighted, diffusion-weighted image acquired (b = 0). A gradient table was used for subsequent data analysis, computed using parameters of the diffusion-weighted images.24
MR Data Processing
Diffusion-weighted images (Figure 1A) were visually inspected for excessive motion artifact or instrumental noise; if an image was deemed corrupt, it was removed before tensor calculation.25 Less than 1% of images were removed across all subjects. After tensor calculation, FA maps were produced on the basis of the magnitude of diffusivity in 3 defined orientations and the mean diffusivity of each within a given tensor.5 Color-coded orientation maps were used to visualize the principal fiber orientation within each pixel (red: right–left; blue: superior–inferior; green: anterior–posterior).
The ROIEditor software program (www.MRIStudio.org) was used to perform manual quantification of PLIC for each participant in subject space. The PLIC was delineated bilaterally, beginning at the level of the anterior commissure and terminating at the inferior border of the corona radiata14 (Figure 1B and C). The regions of interest were delineated using the FA and color maps and a white matter atlas.26 Lesions penetrating the PLIC were not excluded from the drawing procedure. Complete disruption of the affected PLIC was not observed for any participant. Mean FA values for the delineated region were computed by removing pixels with zero or negative FA values; the mean FA from the remaining voxels within the manually defined regions of interest mask was used for statistical analyses. We have previously shown that this approach has good reproducibility in individuals with stroke and healthy participants.7,16
A 2-way (group × day) repeated measures analysis of variance was conducted to evaluate the differences in motor skill performance (ie, RMSE) between the participants with stroke and healthy participants. The groups were then evaluated separately to measure the relationship between skill performance change and posttraining FA values within the PLIC bilaterally. A 1-way (group) multiple analysis of variance was conducted to assess differences in ipsilesional and contralesional posttraining PLIC FA values in the stroke group compared with the nondominant and dominant PLIC in the control group.
Hierarchical multiple linear regression analyses were performed to assess the amount of variance in RMSE change scores explained by PLIC FA values and demographic information. In the stroke group, poststroke duration and age were entered together first into the model to account for stroke chronicity27–29 and age-related changes in learning capacity30 and white matter status.31 Posttraining ipsilesional PLIC FA was the next predictor entered into the model, based on findings suggesting that ipsilesional tract integrity is related to upper limb function,14 followed by contralesional posttraining PLIC FA. In the healthy group, the same order of entry was used with age entered first into the model followed by nondominant (trained) posttraining PLIC FA and then dominant (untrained) posttraining PLIC FA. We conducted the same analyses using hemispheric posttraining PLIC FA asymmetry [(FAcontra − FAipsi)/(FAcontra + FAipsi)] as a single predictor, where larger positive asymmetry values represented greater within-subject differences in posttraining PLIC microstructural integrity. The analyses were also conducted to evaluate the relationship between naïve skill performance (day 1) and post training FA values.
Zero-order, part, and partial correlations were produced for each model. Colinearity between multiple predictors was evaluated using variance inflation factor and tolerance levels. Values under 10 for variance inflation factor and above 0.1 for tolerance were critical levels for acceptable colinearity.32 Residual statistics and plots were produced to ensure normality and homoscedasticity of the data.32,33 Durbin-Watson diagnostics were provided to ensure independence of the residuals. Influential cases and extreme outliers were determined using Cook's distance (>1), studentized residuals (>3), and Mahalanobis distance (>10).32 No extreme outliers were detected. For each statistical test, the significance level was set at P < 0.05. All statistical procedures were conducted using SPSS software (SPSS 19.0).
Comparisons Between Healthy and Chronic Stroke Individuals
Across sessions, healthy participants demonstrated superior skill performance (indicated by lower RMSE values) than participants with stroke (main effect of the group; F(1,20) = 6.09; P = 0.023). Examining the change in skill across training, a group by time interaction was not observed (F(1,20) = 0.38; P = 0.545). Both groups benefited significantly from practice as shown by reduced RMSE values from day 1 to the retention test on day 7 (main effect of time; F(1,20) = 33.60; P < 0.0005) (Figure 2). Results from the multiple analysis of variance demonstrated differences in posttraining FA between groups (Pillai's trace: F(2,19) = 4.34; P = 0.028). This was attributed to difference in posttraining FA in the ipsilesional PLIC. Posttraining mean FA values in the contralesional PLIC did not differ between groups (F(1,20) = 2.24; P = 0.151); however, posttraining mean FA values for the ipsilesional PLIC were lower in the stroke group than in healthy controls (F(1,20) = 7.85; P = 0.011) (Figure 3).
Associations Between Visuomotor Skill Performance and Diffusion Measures
Results of the regression analyses conducted for both groups are summarized in Table 2. In the stroke group, the initial model (poststroke duration, age) did not alone explain a significant amount of variance in change in RMSE associated with skill training or baseline skill performance. Adding posttraining ipsilesional PLIC FA values produced a model that explained a unique amount of variance in response to skill training (R2 = 0.649; F(3,9) = 5.55; P = 0.020; βipsiFA = −1.073) but was not associated with baseline performance. Individuals that demonstrated greater improvements associated with practice (indicated by larger negative RMSE change scores) had higher posttraining ipsilesional PLIC FA values. The bivariate relationship between change in RMSE and posttraining ipsilesional PLIC FA is illustrated in Figure 4A. Note that when the amount of variance in change in RMSE explained by age and poststroke duration is accounted for, posttraining ipsilesional PLIC mean FA explains a larger proportion of variance in performance improvement (reduced RMSE) after training (Figure 4B). Adding posttraining contralesional PLIC FA to the model did not explain an additional amount of unique variance in change in performance; however, the overall model remained significant (R2 = 0.746; F(4,8) = 5.89; P = 0.016).
In a separate model, adding posttraining FA asymmetry as a predictor of change in motor skill learning produced a significant model (R2 = 0.573; F(3,9) = 4.03; P = 0.045; βFA asymmetry = 0.984) (Figure 5A and B), where less asymmetry in posttraining PLIC FA was associated with greater change in motor skill after training. No association was observed with baseline tracking performance for posttraining FA asymmetry (P > 0.05).
In the healthy group, regression analyses did not demonstrate a significant association between posttraining FA values with change in skill or baseline performance (P > 0.05) (Table 2) (Figure 6).
Consistent with past work, our results demonstrate that both individuals with stroke and age-matched healthy controls benefit from skilled practice and can demonstrate motor learning of a novel motor task.2,34 However, our data extend past work showing that magnitude of motor learning-related change in the performance of a complex visuomotor task is associated with posttraining ipsilesional PLIC status, indexed by FA, in individuals with chronic stroke. Interestingly, baseline motor performance was not related to posttraining ipsilesional PLIC FA values, suggesting that white matter status within this region is linked to change in skilled motor behavior rather than naïve motor skill performance.
Numerous studies have shown that given adequate doses of practice individuals with stroke can learn new motor skills using either their hemiparetic1,2,35 or nonhemiparetic arm.3,36–39 Yet, the magnitude of change associated with motor learning after stroke is highly variable, and motor performance after stroke is rarely as accurate or as fast as that of age-matched healthy controls. Our findings suggest that posttraining FA within the ipsilesional PLIC may be an important marker of motor learning-related change in chronic stroke. It has begun to be considered in motor learning studies in healthy controls. When healthy individuals practice a new motor task, a strong relationship exists between skill development and baseline, pretraining FA within the corticospinal tract.40 Thus, future work may need to account for ipsilesional PLIC FA to individualize and optimize motor learning after stroke.
Taken together with other work,14,17,20 our data show the necessity for a certain level of residual white matter tissue to demonstrate motor learning-related behavioral change after stroke. In fact, our data suggest that after stroke behavioral capacity for learning-related change scales with posttraining FA. Although it is possible that training altered FA values, our results show that individuals with higher posttraining FA in the ipsilesional PLIC demonstrated greater magnitude of motor learning-related change (Figure 4A).
Importantly, age and time poststroke alone were not significantly associated with motor learning-related change in behavior; only when these factors were combined with posttraining ipsilesional PLIC FA did the model explain a significant amount of variance in change in skill. This finding indicates that age and poststroke duration should not be used in isolation to determine potential for motor learning-related change. This is important information for individuals who design the content and course of rehabilitation interventions. A recent systematic review supports this contention and has determined there is inconclusive evidence that time since stroke influences upper limb motor recovery.41 It has also been suggested that motor outcomes after stroke may be best predicted by diffusion-based information.6 Furthermore, we propose that altered white matter microstructural integrity within the ipsilesional PLIC may be one source of variability that could account for some degree of between-participant differences in outcomes across motor learning studies. This factor may need to be accounted for in the design and interpretation of experiments intended to ascertain the optimal choice of rehabilitation intervention, dose of practice, and feedback schedule to stimulate motor learning after stroke.
Our data confirm and extend those of Stinear et al14 showing that motor learning-related change is associated with FA asymmetry between ipsi- and contralesional PLIC, where an asymmetry greater than 0.25 was associated with lower potential for functional recovery and reduced response to an experimental block-moving task. Given that even after a unilateral stroke white matter in both PLICs and motor function in both arms may be disrupted, indexing FA asymmetry provides a measure of white matter status that more fully characterizes upper extremity motor function. Interestingly, examination of our results shows that individuals with asymmetry of 0.20 or higher failed to demonstrate motor learning-related change (Figure 5A). It may be that the greater complexity and motor control requirements of our visuomotor pursuit motor learning task necessitated more symmetry between ipsi- and contralesional posttraining PLIC FA values as compared with the relatively simple block-moving task employed in past work. Importantly, it is consistently clear in the literature that greater asymmetry in PLIC FA after stroke contributes to both increased levels of physical impairment (reduced FM scores)14,15 and reduced motor learning capacity.
There are limitations to this study. The sample of participants with stroke was relatively small and homogenous. Although this homogeneity may improve sensitivity to observing effects by reducing intersubject variability, it could also limit its generalizability to all stroke populations. Sample size may have also reduced statistical power required to detect associations between white matter integrity and baseline skill performance. The sample size studied may also limit generalizability of the results from the multiple regression analyses. Future work should evaluate our preliminary findings in a larger participant cohort. Furthermore, we were not powered to detect relationships between our variables within the healthy control group. Diffusion imaging was limited to 1 scan with a limited number of orientations, yet this is comparable to related investigations.14,15,42 However, our scan parameters may have decreased the signal-to-noise ratio and resolution, thus decreasing sensitivity to detecting small changes in diffusion behavior, especially at tissue boundaries and brain regions with crossing fibers. We focused on evaluating 1 DTI-based metric of white matter in the PLIC in an effort to minimize these potential limitations. We also limited our investigation to the PLIC because it was a region previously shown to have reduced integrity after stroke.14,15 Other investigations have used alternative analysis techniques to evaluate this subsection of tract and also other white matter regions within the brain.42–44 Currently there is no gold standard for DTI analysis techniques, and all approaches have potential limitations and pitfalls45 that need to be considered when determining optimal imaging and analysis parameters in stroke.
Another limitation of this study was that diffusion imaging was performed after training. Currently, the time required to observe training-induced white matter plasticity in healthy individuals is unclear. Studies showing change in white matter diffusion behavior after training generally occur over 5 to 8 weeks.9,10,13 Furthermore, in a study specifically related to motor-based training, healthy individuals were trained on a serial reaction time motor skill task for 10 days within only a 2-week period and showed no FA differences after training.46 However, a recent investigation showed that only 2 hours of training on a spatial memory and learning motor task produced short-term changes in hippocampal and parahippocampal white matter.47 Only 1 research report has documented white matter change in individuals with stroke; this work considered the impact of a functional electrical stimulation intervention on mean FA values.48 This work included only 3 participants and has yet to be replicated. Finally, DTI has not shown sensitivity to detect small, positive changes in white matter microstructure (ie, increases in FA) that are likely associated with laboratory-based motor learning studies in older individuals or in people with chronic stroke; this result may stem in part from the relatively low doses of practice delivered in most motor learning studies.2,3,31,34 A newer imaging modality called multicomponent T2 relaxation imaging (MCRI) has recently been shown to index a specific element of white matter microstructure, myelin, in individuals with chronic stroke.49 Although MCRI may allow the quantification of motor learning-related change in white matter microstructure after stroke, it has yet to be fully investigated in this context. Thus, given the issues outlined previously, for this study, we chose not to attempt to index change in FA but instead focused on understanding if posttraining white matter tissue status might be associated with motor learning-related change in behavior. Further work including longitudinal studies of training and white matter plasticity, which employs imaging techniques that are both sensitive to change and specific to brain structure such as MCRI, will be required to determine whether it is possible that training-induced white matter plasticity supports the acquisition of new motor skills.
Finally, this investigation focused on 1 type of skill learning and a single training paradigm. Repeated daily practice sessions performing continuous tracking with the affected extremity have previously demonstrated implicit motor skill learning in individuals with stroke1 and have important implications for rehabilitation.50,51 Future inquiry should evaluate relationships between adaptive brain structure and function, with response to different motor learning task paradigms using multimodal neuroimaging approaches.
Our findings indicate that posttraining diffusion-based measures of white matter microstructural integrity of ipsilesional primary motor output tracts are closely associated with response to motor skill training in well-recovered individuals with stroke in the chronic phase of recovery. Importantly, demographic information in isolation did not predict motor learning-related change. These results imply that regional white matter diffusion characteristics may be an important factor to consider when interpreting or predicting response to rehabilitation or when designing studies that consider motor learning-related change.
This work was supported by the National Institutes of Health [NS051714 to L.A.B.] and the Canadian Institutes of Health Research [MOP-106651 to L.A.B.]. Support was also provided to L.A.B. by the Canada Research Chairs and the Michael Smith Foundation for Health Research. The Natural Sciences and Engineering Research Council of Canada provided support to K.E.B. and the Heart and Stroke Foundation of Canada supported M.R.B.
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cerebrovascular accident; diffusion tensor imaging; motor behavior; posterior limb of the internal capsule; rehabilitation
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