Skip Navigation LinksHome > June 2014 - Volume 73 - Issue 6 > Mechanisms Involved in Spinal Cord Central Synapse Loss in a...
Journal of Neuropathology & Experimental Neurology:
doi: 10.1097/NEN.0000000000000074
Original Articles

Mechanisms Involved in Spinal Cord Central Synapse Loss in a Mouse Model of Spinal Muscular Atrophy

Tarabal, Olga PhD; Caraballo-Miralles, Víctor PhD; Cardona-Rossinyol, Andrea MSc; Correa, Francisco J. MSc; Olmos, Gabriel PhD; Lladó, Jerònia PhD; Esquerda, Josep E. MD, PhD; Calderó, Jordi MD, PhD

Collapse Box


Abstract: Motoneuron (MN) cell death is the histopathologic hallmark of spinal muscular atrophy (SMA), although MN loss seems to be a late event. Conversely, disruption of afferent synapses on MNs has been shown to occur early in SMA. Using a mouse model of severe SMA (SMNΔ7), we examined the mechanisms involved in impairment of central synapses. We found that MNs underwent progressive degeneration in the course of SMA, with MN loss still occurring at late stages. Loss of afferent inputs to SMA MNs was detected at embryonic stages, long before MN death. Reactive microgliosis and astrogliosis were present in the spinal cord of diseased animals after the onset of MN loss. Ultrastructural observations indicate that dendrites and microglia phagocytose adjacent degenerating presynaptic terminals. Neuronal nitric oxide synthase was upregulated in SMNΔ7 MNs, and there was an increase in phosphorylated myosin light chain expression in synaptic afferents on MNs; these observations implicate nitric oxide in MN deafferentation and suggest that the RhoA/ROCK pathway is activated. Together, our observations suggest that the earliest change occurring in SMNΔ7 mice is the loss of excitatory glutamatergic synaptic inputs to MNs; reduced excitability may enhance their vulnerability to degeneration and death.

© 2014 by American Association of Neuropathologists, Inc.


Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.