Skip Navigation LinksHome > January 2012 - Volume 71 - Issue 1 > The Importance of 10q Status in an Outcomes-Based Comparison...
Journal of Neuropathology & Experimental Neurology:
doi: 10.1097/NEN.0b013e318240fa65
Original Articles

The Importance of 10q Status in an Outcomes-Based Comparison Between 1p/19q Fluorescence In Situ Hybridization and Polymerase Chain Reaction–Based Microsatellite Loss of Heterozygosity Analysis of Oligodendrogliomas

Horbinski, Craig MD, PhD; Nikiforova, Marina N. MD; Hobbs, Jonathan BS; Bortoluzzi, Stephanie BS; Cieply, Kathleen MLS; Dacic, Sanja MD, PhD; Hamilton, Ronald L. MD

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Abstract: 1p/19q codeletion is a favorable prognostic marker of oligodendrogliomas. Although fluorescence in situ hybridization (FISH) and microsatellite-based polymerase chain reaction (PCR) for loss of heterozygosity (LOH) are common methods to test for 1p/19q codeletion, it is unclear which test is better at prognostic stratification. This study analyzed outcomes of 111 oligodendrogliomas with both 1p/19q FISH and LOH done at the time of diagnosis. Overall concordance between the 2 assays was 81.1%. In grade III oligodendrogliomas, LOH was better than FISH at survival stratification (p < 0.0001 for LOH vs p = 0.02 for FISH), although increasing the stringency of FISH interpretation criteria improved concordance and prognostic power. Oligodendrogliomas that were 1p/19q-codeleted by FISH but also had 10q LOH were negative for 1p/19q codeletion by PCR analysis in more than 70% of cases, with very poor survival in the grade III subset. Thus, although PCR-based LOH is a better stratifier of 1p/19q status, FISH still has clinical and prognostic utility, especially if 10q data can be incorporated.

© 2012 American Association of Neuropathologists, Inc


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