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Which Lesions Should Be Biopsied During High-Resolution Anoscopy? Prospective Descriptive Study of Simple Morphological Criteria

Camus, Marine MD1; Lesage, Anne-Carole MD1; Fléjou, Jean-Francois MD, PhD2; Hoyeau, Nadia MD2; Atienza, Patrick MD1; Etienney, Isabelle MD1

Journal of Lower Genital Tract Disease: April 2015 - Volume 19 - Issue 2 - p 156–160
doi: 10.1097/LGT.0000000000000064
Original Articles: Perianal and Anal

Objective: High-resolution anoscopy (HRA) is a useful screening tool for anal intraepithelial neoplasia (AIN), although reputedly challenging for interpretation of suspected lesions.

Materials and Methods: All consecutive patients who underwent biopsies for at least 1 lesion suspicious for AIN during HRA from November 2010 to March 2012 were prospectively included. The characteristics (borders, staining with acetic acid and Lugol iodine solution, and patterns of epithelium and vascularization) of the lesions detected during HRA were compared with histology.

Results: A total of 168 suspicious anal lesions were biopsied and analyzed in 103 patients (68% men, mean age ± standard deviation = 49.8 ± 9 y, 57% positive status on human immunodeficiency virus infection). According to histology, 41.7% of the lesions were high grade, 34.5% were low grade, and 23.8% were nondysplastic. Lesions with irregular epithelial pattern (or irregular vascularization) were twice as likely to be high grade compared with lesions with regular epithelial pattern (or regular vascularization). The incidences of acetic acid-induced whitening were 91.4%, 94.8%, and 70% among the high-grade AIN, low-grade AIN, and nondysplastic samples, respectively. Among the high-grade AIN, 62.9% were not stained by Lugol solution (vs 31% of the low-grade AIN). The positive predictive value of a combination of these simple morphological criteria was 68.6%.

Conclusions: Several simple morphological criteria are significantly associated with high-grade AIN and are found less often in low-grade AIN. A combination of these morphological criteria provides sufficient positive predictive value to guide biopsy placement during HRA.

Simple morphological criteria help to recognize anal high-grade intraepithelial neoplasia before biopsy during high-resolution anoscopy.

1Department of Proctology, Diaconesses Hospital, and 2Department of Anatomopathology, University Paris VI, Saint-Antoine Hospital, APHP, Paris, France

Reprint requests to: Isabelle Etienney, MD, Department of Proctology, Diaconesses Hospital, 18 rue du Sergent Bauchat, 75012 Paris, France. E-mail: proctologie@hopital-dcss.org

The authors have declared they have no conflicts of interest.

There is no financial support to declare.

Podium meeting presentation: The American Society of Colon and Rectal Surgeons, Annual Scientific Meeting, Phoenix, AZ; April 27 to May 1, 2013.

Podium meeting presentation: Journées Francophones d’Hépatogastroentérologie et d’Oncologie Digestive, French Gastrointestinal Week, Paris, France; March 16 to 21, 2013.

Copyright © 2015 by the American Society for Colposcopy and Cervical Pathology