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Journal of Lower Genital Tract Disease:
doi: 10.1097/LGT.0b013e3182a7bb89
Original Articles

Molecular Epidemiology of Human Papillomavirus Infections in Cervical Samples From Cuban Women Older Than 30 Years

Soto, Yudira MSc1; Torres, Griselda MSc2; Kourí, Vivian MD, PhD1; Limia, Celia María BSc1; Goicolea, Adibel MD3; Capó, Virginia MD, PhD2; Pérez, Lissette PhD1; de la Torre, Ana Isabel MD4; López, Ledy Xiomara MSc2; Govín, Anamays MSc2; Correa, Consuelo Beatriz MD, MSc1; Alemán, Yoan MSc1; Álvarez, Alina Ana Tech1; Manzano, Blanca Rosa MD, DrSc4

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Objective: This study aimed to provide information about the molecular epidemiology of human papillomavirus (HPV) in a group of Cuban women.

Materials and Methods: DNA from cervical samples was analyzed using a quantitative real-time polymerase chain reaction (PCR), which detects 6 of the clinically most relevant high-risk HPV types. Furthermore, end point PCR and sequencing were performed. Three hundred twenty-two women (211 with positive and 111 with negative cytologic results) aged between 30 and 69 years were enrolled. Risk factors associated with HPV infections and premalignant lesions were also investigated.

Results: HPV DNA was detected in 76.1% (245/322) of the studied population, and 34 different genotypes were found. There was an association between HPV infection and low educational level, history of oral contraceptives, menopausal stage, as well as cigarette and/or alcohol consumption. Besides, in a multivariate analysis, previous positive Pap test result and positive colposcopy finding were both predictor variables for HPV infections and for premalignant lesions. Human papillomavirus infection was found in 94.3% of women (199/211) with positive cytologic result and in 41.4% (46/111) of those with negative results, being more likely that the first group was infected with any HPV (odds ratio = 23.43; 95% CI = 11.70–46.92; p = .000). The most common genotypes were HPV types 16, 18, 31, 58, 33, and 45. All the cases with HPV positive findings had at least 1 high-risk HPV genotype.

Conclusions: This is the first report of the molecular epidemiology of HPV in Cuban women, based on results from a DNA sequence and quantitative PCR. Most individuals were infected with high-risk HPV types. These findings support the inclusion of HPV vaccine in Cuba.

Copyright © 2014 by the American Society for Colposcopy and Cervical Pathology


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