Skip Navigation LinksHome > February 2014 - Volume 62 - Issue 2 > Effect of Prostaglandin I2 Analogs on Macrophage Inflammator...
Journal of Investigative Medicine:
doi: 10.231/JIM.0000000000000042
Original Articles

Effect of Prostaglandin I2 Analogs on Macrophage Inflammatory Protein 1α in Human Monocytes Via I Prostanoid Receptor and Cyclic Adenosine Monophosphate

Tsai, Ming-Kai MD*†; Hsieh, Chong-Chao MD; Kuo, Hsuan-Fu MD§; Yang, San-Nan MD, PhD∥¶; Kuo, Chang-Hung MD**††‡‡; Huang, Ming-Yii MD, PhD§§; Tsai, Ying-Ming MD∥∥; Lee, Min-Sheng MD††‡‡; Hung, Chih-Hsing MD, PhD**††‡‡¶¶

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Abstract

Aims

Inflammation plays critical roles in atherosclerosis. Chemokines are responsible for leukocyte trafficking and involve in inflammatory diseases. Macrophage inflammatory protein 1α (MIP-1α) has been implicated in atherosclerotic lesion formation. Prostaglandin I2 (PGI2) analog, used in pulmonary hypertension, has been reported to have anti-inflammatory functions. However, little is known about its role in the MIP-1α production in human monocytes.

Methods

We investigated the effects of 3 conventional (iloprost, beraprost, and treprostinil) and 1 new (ONO-1301) PGI2 analogs, on the expression of MIP-1α expression in human monocytes. Human primary monocytes from control subjects and THP-1 cell line were treated with PGI2 analogs, with or without lipopolysaccharide (LPS) stimulation. Supernatants were harvested to measure MIP-1α levels by enzyme-linked immunosorbent assay. To explore which receptors involved the effects of PGI2 analogs on the expression of MIP-1α expression, I prostanoid (IP) and E prostanoid, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-r receptor antagonists were used to pretreat THP-1 cells. Forskolin, a cyclic adenosine monophosphate (cAMP) activator, was also used to further confirm the cAMP involvement on the effect of PGI2 analogs in MIP-1α production.

Results

Three PGI2 analogs could suppress LPS-induced MIP-1α production in THP-1 cells and human primary monocytes. ONO-1301 had a similar effect. CAY 10449, an IP receptor antagonist, could reverse the suppressive effects on MIP-1α production of iloprost. Forskolin, a cAMP activator, also suppressed MIP-1α production in THP-1 cells.

Conclusions

Prostaglandin I2 analogs suppressed LPS-induced MIP-1α production in human monocytes via the IP receptor and cAMP pathway. The PGI2 analog may be potential in the treatment for atherosclerosis.

Copyright © 2014 by The American Federation for Medical Research

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