Skip Navigation LinksHome > March 2013 - Volume 61 - Issue 3 > MicroRNA-30b-5p Is Involved in the Regulation of Cardiac Hyp...
Journal of Investigative Medicine:
doi: 10.231/JIM.0b013e3182819ac6
Original Articles

MicroRNA-30b-5p Is Involved in the Regulation of Cardiac Hypertrophy by Targeting CaMKIIδ

He, Juan MS; Jiang, Shan MS; Li, Feng-lan MS; Zhao, Xue-jiao MS; Chu, Er-fu MS; Sun, Mei-na MS; Chen, Ming-zi MS; Li, Hui PhD

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Abstract

Background: MicroRNAs (miRNAs) participate in the regulation of cardiac hypertrophy. However, it remains largely unknown as to how miRNAs are integrated into the hypertrophic program. Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a hypertrophic signaling marker. It is not yet clear which miRNAs can regulate CaMKIIδ.

Purpose: In this study, we identified which miRNAs could regulate CaMKIIδ and how to regulate CaMKIIδ.

Methods: Through computational and expression analyses, miR-30b-5p was identified as a candidate regulator of CaMKIIδ. Quantitative expression analysis of hypertrophic models demonstrated significant down-regulation of miR-30b-5p compared with control groups. Luciferase reporter assay showed that miR-30b-5p could significantly inhibit the expression of CaMKIIδ. Moreover, through gain-of-function and loss-of-function approaches, we found miR-30b-5p could negatively regulate the expression of CaMKIIδ and miR-30b-5p was a regulator of cardiac hypertrophy.

Conclusion: Our study demonstrates that the expression of miR-30b-5p is down-regulated in cardiac hypertrophy, and restoration of its function inhibits the expression of CaMKIIδ, suggesting that miR-30b-5p may act as a hypertrophic suppressor.

© 2013 American Federation for Medical Research

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