Skip Navigation LinksHome > December 2011 - Volume 59 - Issue 8 > Quantitative Assessment of Wilms Tumor 1 (WT1) Gene Transcri...
Journal of Investigative Medicine:
doi: 10.231/JIM.0b013e31822a24f7
Original Articles

Quantitative Assessment of Wilms Tumor 1 (WT1) Gene Transcripts in Egyptian Acute Lymphoblastic Leukemia Patients

Ali Sadek, Hoda MD*; El-Metnawey, Wafaa Hassan MD; Shaheen, Iman Abdel-Mohsen MD*; Korshied, Mervat Mamdouh MD*; Mohamed, Azza Sobieh MD

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Abstract

Background: Accurate assessment of minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL) patients after initial chemotherapy is essential to evaluate the efficacy of therapeutic regimens. Wilms tumor 1 (WT1) is a pan-leukemic marker used for identification of the leukemic clone rather than the use of individual specific molecular aberration of ALL.

Methods: Using a real-time quantitative polymerase chain reaction, bone marrow samples from 41 newly diagnosed Egyptian ALL patients; 22 adults and 19 children were examined for WT1 expression. After induction therapy, WT1 expression was reestimated in 20 ALL patients.

Results: WT1 was overexpressed in adult and pediatric ALL patients (95.4% and 89.4%, respectively). WT1 expression at diagnosis had no statistically significant impact on disease-free survival of patients (P = 0.054). However, WT1 expression increased after induction chemotherapy in the 3 pediatric patients who had relapse.

Conclusions: WT1 is a leukemia-associated molecular marker that may be used for the diagnosis and for monitoring clinical progress in ALL; it also can be used as a molecular target for adoptive immunotherapy.

© 2011 American Federation for Medical Research

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