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O-Linked Mannose -1,2-N-acetylglucosaminyltransferase 1 Correlated With the Malignancy in Glioma

Lan, Jin PhD*; Guo, Pin PhD*; Chen, Mo BS; Wu, Bin*; Mao, Qing MD, PhD*†; Qiu, Yongming MD, PhD*†

Journal of Craniofacial Surgery: July 2013 - Volume 24 - Issue 4 - p 1441–1446
doi: 10.1097/SCS.0b013e318295378b
Scientific Foundations

Abstract: O-linked mannose β-1,2-N-acetylglucosaminyltransferase 1 (PomGnT1) constitutes one third of the O-linked glycoproteins in brain tissue. However, its functions have been seldom investigated in brain cancers. In this study, immunohistochemistry was used for the detection of the PomGnT1 protein in 133 cases of glioma tissues. Spearman correlation analysis was used for the relationship between PomGnT1 staining and the glioma grade. Receiver operating characteristic curve was used to measure the diagnostic value of PomGnT1 protein in the degree of glioma malignance. We found that PomGnT1 expression was correlated with glioma grade, and it could be used as a marker to distinguish low- and high-grade gliomas. Stably transfected U87 cells were constructed to overexpress short hairpin RNA of PomGnT1. Immunofluorescence test detected that this protein also could restrain the generation of U87 cells’ pseudopodia. Western blotting further showed that the PomGnT1 protein had an impact on the c-myc protein level. In conclusion, our data suggest that PomGnT1 protein was correlated with the malignance of glioma progression, the mechanism involved in glioma cell’s pseudopodium formation, and the expression of c-myc protein.

From the *Department of Neurosurgery, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine; †Shanghai Institute of Head Trauma; and ‡Renji Clinic Medical School, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Received February 25, 2013.

Accepted for publication March 31, 2013.

Address correspondence and reprint requests to Yongming Qiu, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 1630 Dongfang Rd, Shanghai 200127, China; E-mail: qiuzhoub@hotmail.com or Qing Mao, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 1630 Dongfang Rd, Shanghai 200127, China; E-mail: maoq@netease.com

This work was supported by a project of building hospital clinical key disciplines (RJ.4101307) and by the National Natural Science Foundation of China (30901738).

Ethical approval: All experiments and protocol of this study were approved by the Ethics Committee of Renji Hospital, Shanghai Jiaotong University School of Medicine.

The authors report no conflicts of interest.

© 2013 by Mutaz B. Habal, MD.