Journal of Craniofacial Surgery

Skip Navigation LinksHome > July 2012 - Volume 23 - Issue 4 > Impact of Smoking on p65 Nuclear Factor κB, p38 Mitogen-Acti...
Journal of Craniofacial Surgery:
doi: 10.1097/SCS.0b013e31824e2c34
Original Articles

Impact of Smoking on p65 Nuclear Factor κB, p38 Mitogen-Activated Protein Kinase, and Inducible Nitric Oxide Synthase Expression Levels in Oral Mucosa

Meric, Aysenur MD*; Ozucer, Berke MD*; Gedikli, Orhan MD; Yildirim, Yalcin MD*; Korkut, Arzu Yasemin MD; Kahya, Volkan MD*; Eren, Sabri Baki MD*; Somay, Adnan MD§

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Objectives: Smoking plays an important role in oral cancer development; however, the molecular carcinogenesis mechanism in oral mucosa is not well understood. The aim of this study was to examine and compare the levels of p65 nuclear factor κB (NF-κB), p38 mitogen-activated protein kinase (MAPK), and inducible nitric oxide synthase (iNOS) expressions between oral mucosa of nonsmoker and smoker volunteers.

Methods: Oral cheek mucosa was collected from 78 volunteers. Smokers were divided into 2 subgroups: light smokers (<40 pack years) and heavy smokers (≥40 pack years). Paraffinized tissue immunochemistry was carried out for p65 NF-κB, p38 MAPK, and iNOS expression with specific antibodies. Results were evaluated based on diffuseness and intensity of staining.

Results: Group 1 composed of 40 nonsmokers: 52.5% were female and 47.5% were male, with a mean age of 46.4 years. Group 2 composed of 38 smokers (20 light smokers, 18 heavy smokers): 39.5% were female and 60.5% were male, with a mean age of 48.9 years. Total immunohistochemical staining scores of smokers were significantly higher compared with those of nonsmokers in p65 NF-κB, p38 MAPK, and iNOS expression (P < 0.001). The highest p65 NF-κB, p38 MAPK, and iNOS expression levels were detected in the oral mucosa of heavy smokers. The expression of iNOS and p65 NF-κB in heavy smokers was significantly higher compared to that in light smokers (P < 0.01 and P < 0.05, respectively). Although p38 MAPK expressions were higher in heavy smokers compared with light smokers, the difference was not statistically significant (P > 0.05).

Conclusions: Our results show for the first time the significant increase in the expression of p65 NF-κB and p38 MAPK in the oral mucosa of smokers. Levels of p65 NF-κB, p38 MAPK, and iNOS expression in the oral mucosa of smokers were related to the number of pack years.

© 2012 Mutaz B. Habal, MD

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