Skip Navigation LinksHome > September 2011 - Volume 22 - Issue 5 > Effects of Irradiation on Growth and Differentiation-Related...
Journal of Craniofacial Surgery:
doi: 10.1097/SCS.0b013e31822e5f66
Original Articles

Effects of Irradiation on Growth and Differentiation-Related Gene Expression in Osteoblasts

He, Jie PhD*; Qiu, Weiliu MD, PhD*; Zhang, Zhiyuan MD, PhD*; Wang, Zhonghe MD†; Zhang, Xiuli MD*; He, Yue MD, PhD*

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Abstract

Osteoblasts are bone-forming cells that are responsible for the production of bone extracellular matrix. Osteoradionecrosis is a complication of radiation therapy for carcinoma of the head and neck that occurs in 3% to 8.2% of irradiated patients. The irradiation effect on osteoblast differentiation has not been fully elucidated. The objective of our research was to elucidate the effects of radiation on the growth and differentiation-related gene expression in osteoblast in vitro. Three differentiation-related genes, alkaline phosphatase, type I collagen, and osteocalcin, were tested in our experiment. The results showed that radiation inhibited the proliferation of the osteoblasts in a dose-dependent manner and induced G2/M cell cycle arrest. Irradiation, 4 and 8 Gy, enhanced the differentiation-related gene expression of MC3T3-E1 cells 7 days after irradiation. However, the differentiation-related gene expression was decreased 21 days after irradiation in the 4- and 8-Gy groups. This work presents the dynamic phenotypic expression changes of osteoblastic cells after x-ray irradiation.

© 2011 Mutaz B. Habal, MD

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