This study involved a histologic, enzyme histologic, immunohistologic, and three-dimensional microstructure evaluating the extent of osteogenesis and repair in the human alveolar extraction socket achievable with an artificial bone substitute. After tooth extraction in 7 patients, extraction sockets were filled with Mastergraft (15% hydroxyapatite, 85% β-tricalcium phosphate complex). Radiomicrographs and histologic examinations were performed on samples obtained during dental implant placement procedure. On micro-computed tomography, new bone was observed in all collected samples, and osteogenesis was observed to have taken place around the artificial bone substitute. Histologically, active osteogenesis was found throughout the region observed. Addition of new bone around the Mastergraft was observed, and osteoblast-like cells were present. Cells that had partially invaded the artificial bone included tartrate-resistant acid phosphate-positive and CD34-positive cells. These findings indicate that the Mastergraft artificial bone induced osteogenesis in the jawbone and seemed effective for repairing bone defects.
From the *Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan; †Surgical Implant Center, University of California at Los Angeles, School of Dentistry, Los Angeles, California; ‡Department of Oral and Maxillofacial Surgery, Fukui University Medical School, Fukui, Japan; §SAR, Department of Material and Life Science, Graduate School of Engineering, Osaka University, Suita, Japan; and ∥Department of Oral and Maxillofacial Surgery, University of California at Los Angeles, School of Dentistry, Los Angeles, California.
Received July 5, 2010.
Accepted for publication September 25, 2010.
Address correspondence and reprint requests to Mari Wakimoto, DDS, Department of Oral and Maxillofacial Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata, Kita-ku, Okayama 7008525, Japan; E-mail: email@example.com
The authors report no conflicts of interest.