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JAIDS Journal of Acquired Immune Deficiency Syndromes:
doi: 10.1097/QAI.0b013e3182874d41
Brief Report: Basic and Translational Science

Antibody-Dependent Cell-Mediated Virus Inhibition Antibody Activity Does Not Correlate With Risk of HIV-1 Superinfection

Forthal, Donald N. MD*; Landucci, Gary BS*; Chohan, Bhavna PhD†,‡,§; Richardson, Barbra A. PhD†,‖; McClelland, R. Scott MD, MPH; Jaoko, Walter MBChB; Blish, Catherine MD, PhD§,¶; Overbaugh, Julie PhD§

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*Division of Infectious Diseases, Department of Medicine, University of California, Irvine School of Medicine, Irvine, CA;

Department of Global Heath, University of Washington, Seattle, WA;

Department of Medical Microbiology, University of Nairobi, Nairobi, Kenya

§Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, WA;

Department of Biostatistics, University of Washington, Seattle, WA; and

Blish is now with the Division of Infectious Diseases and Geographic Medicine, Department of Medicine and Stanford Immunology, Stanford University School of Medicine, Stanford, CA.

Correspondence to: Donald N. Forthal, Division of Infectious Diseases, Department of Medicine, 3044 Hewitt Hall, University of California, Irvine School of Medicine, Irvine, CA 92697 (e-mail:; or to: Julie Overbaugh, Division of Human Biology, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N. Seattle, WA 98109-1024 (email:

Supported by National Institutes of Health grant R37-AI038518.

D. N. Forthal drafted the paper and designed the ADCVI experiments; G. Landucci conducted the ADCVI experiments; B. Chohan identified cases of superinfection and the timing of superinfection; B. A. Richardson provided statistical analyses; C. Blish and J. Overbaugh designed the case–control study; R. S. McClelland and W. Jaoko provided clinical data and samples; and J. Overbaugh developed the overall concept and oversaw the study.

The authors have no conflicts of interest to disclose.

Received December 08, 2012

Accepted January 10, 2013

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Abstract: Previous studies of HIV-infected women with high-risk behavior have indicated that neither neutralizing antibody nor cellular immunity elicited by an initial HIV-1 infection is associated with protection against superinfection with a different HIV-1 strain. Here, we measured antibody-dependent cell-mediated virus inhibition (ADCVI) antibody activity in the plasma of 12 superinfected cases and 36 singly infected matched controls against 2 heterologous viruses. We found no association between plasma ADCVI activity and superinfection status. ADCVI antibody activity against heterologous virus elicited by the original infection may not contribute to preventing a superinfecting HIV-1.

Individuals with chronic HIV-1 infection can become infected with additional strains of HIV-1 upon sexual exposure.1 In some high-risk populations, such superinfections occur at a rate approaching that of primary infection, calling into question the ability of virus-elicited immunity to prevent further infection.2–5 This question has been directly investigated in a comparison of neutralizing antibody activity in plasma of superinfected and singly infected women from Mombasa, Kenya.6,7 In that study, no significant differences were found in the breadth or potency of HIV-1–specific neutralizing antibody responses between the singly and the superinfected women in specimens obtained just before the time of superinfection. Further investigation in this same cohort of women found that cellular immune responses, measured by the frequency of HIV-1–specific cytokine-producing or cytolytic CD8+ or CD4+ T cells, were not significantly associated with the odds of superinfection.8

To explore further the possible role of immunity induced by the initial infection on acquiring a second infection, we measured antibody-dependent cell-mediated virus inhibition (ADCVI) activity in plasma from the Mombasa cohort. ADCVI is a measure of Fcγ receptor (FcγR)–mediated antiviral activity and occurs when antibody bound to virus-infected target cells engages FcγR-bearing effector cells, such as natural killer cells, monocytes, or macrophages. The virus inhibition that results is due in part to target cell death (antibody-dependent cellular cytotoxicity) and in part to noncytolytic mechanisms, such as β-chemokine release from the effector cells. ADCVI antibody activity has been associated with protection from lentivirus infections in macaques.9 In addition, higher ADCVI activity in serum from healthy individuals vaccinated with recombinant gp120 in the Vax004 trial was associated with a lower rate of sexually acquired HIV infection.10

The cohort of subjects for this study has been described in detail elsewhere and consisted of 12 women with superinfection (cases) and 3 singly infected women matched to each superinfected case (controls; n = 36) according to HIV-1 subtype, timing of samples in relation to initial infection, and viral load.6,8 The median estimated time between sample collection and superinfection was 102 days (range 13–573). All subjects were from Mombasa, Kenya, and were infected through heterosexual contact; none used antiretroviral therapy during the study period. Women were classified as superinfected or singly infected based on phylogenetic analyses of env and gag sequences. The ethical review committees of the University of Nairobi, the University of Washington, the Fred Hutchinson Cancer Research Center, and the University of California, Irvine, approved this study.

Because subtype A is the major subtype in the population,11 2 subtype A HIV-1 variants were used to infect target cells for the ADCVI assays. The 2 replication-competent viruses were derived from proviral clones that were isogenic in all genome regions except envelope. Q23XhoΔXho is a full-length molecular clone obtained from a sexually infected woman at 142 days postinfection, and the BS208.B1 chimeric provirus was generated from Q23XhoΔXho by replacing the envelope with one derived from a vertically infected infant at the first time of documented infection (6 weeks of life).12 These strains were chosen from a panel of clade A molecular clones based on their ability to grow well in our CEM.NKR-CCR5 target cells. In addition, Q23XhoΔXho and BS208.B1 were felt to be representative of other strains because ADCVI results using Q23XhoΔXho and BS208.B1 correlated with results using the other strains.

The ADCVI assay has been described previously.10,13 Briefly, target cells, consisting of CEM.NKR-CCR5 cells (National Institutes of Health AIDS Research and Reference Reagent Program), were infected with either of the viral strains for 72 hours and then washed to remove cell-free virus. Next, effector cells (peripheral blood mononuclear cells from healthy donors) and a 1:100 dilution of test plasma from cases or controls were added to the infected target cells. The effector:target ratio was 10:1. After 3 days, supernatant fluid bathing the target and effector cells was replaced with fresh medium after washing 5 times. After an additional 4 days (total of 10 days after infecting target cells), the supernatant fluid was assayed for p24 by enzyme-linked immunosorbent assay (Zeptometrix, Minneapolis, MN). The percent virus inhibition was calculated by comparison with negative control samples as previously described. The ADCVI assay was conducted with 2 different peripheral blood mononuclear cell effector cell donors for each virus strain. Thus, a total of 4 different donors were used; for each donor, the ADCVI assay was run in triplicate and mean percent virus inhibition is reported.

Plasma ADCVI antibody activity ranged from 0% to >95% irrespective of virus strain or effector cell donor. Although absolute ADCVI activity for each virus differed between experiments with different effector cell donors, there was a strong correlation between the results from the 2 individual donor effector cells when assayed with the same virus (Spearman rho = 0.8, P < 0.0001 for both viruses, Fig. 1). Thus, despite effector cell donor-to-donor variability, the relative antibody activity of plasma stayed similar in repeated assays.

Figure 1
Figure 1
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These results demonstrate the marked variability in the ADCVI antibody response among infected individuals. We have shown previously that vaccination of healthy individuals with recombinant gp120 also elicits a wide range of ADCVI antibody responses.10 Although the factors underlying this variability are not clear, Fc–FcγR interactions, which are required for ADCVI, are highly dependent on immunoglobulin G subclass and on the pattern of Fc glycosylation. Thus, genetic or environmental factors that determine immunoglobulin G subclass responses to infection and vaccination and that influence Fc glycans are likely to be important.

We compared ADCVI activity, as a continuous variable, between superinfected cases and singly infected controls. For target cells infected with the BS208.B1 virus, cases did not differ from controls in either of the 2 experiments using different effector cell donors (P = 0.98 and P = 0.48, Friedman test, Fig. 2A). Similarly, there were no differences between cases and controls in either of the experiments against target cells infected with the Q23XhoΔXho virus (P = 0.17 and P = 0.61, Friedman test, Fig. 2B). We had previously shown that individuals with the highest quartile of ADCVI responses (≥90% virus inhibition) to recombinant gp120 vaccination in the Vax004 trial had almost half the infection rate as those in the lowest quartile.10 Therefore, we conducted separate analyses using ADCVI data bifurcated at 90%, 80%, or 70% virus inhibition. We also quartilized virus inhibition. In all these generalized estimating equations logistic models, there was no association between the ADCVI activity and the odds of a superinfection (data not shown).

Figure 2
Figure 2
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These results indicate that the ADCVI antibody response to a primary infection with HIV-1 may not prevent infection after exposure to additional strains. Of particular note, 3 of the 12 superinfected women had high ADCVI activity (ranging from 82% to >99% virus inhibition) against both viruses tested. Because a study of this size required that we use heterologous virus as a surrogate for the measurement of protective ADCVI responses, it remains possible that the viruses that established superinfection were not recognized by the relatively potent heterologous responses that we measured. We also cannot rule out the possibility that some of the women who were not superinfected do, in fact, have effective immune responses against strains that were successfully inhibited. In addition, in vivo ADCVI activity depends on the presence of well-functioning FcγR-bearing effector cells presumably at or near sites of exposure. We did not measure effector cell function in this cohort, and individuals who are chronically infected are likely to have defects in FcγR-mediated immune functions.7,14–16 Thus, despite broad and potent ADCVI antibody activity, antiviral activity might be ineffective in vivo. Nonetheless, our results, together with those from some6,8,17 but not other studies,18,19 provide further evidence that immunity engendered by a primary HIV infection may do little to prevent a subsequent infection upon sexual exposure to another strain.

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1. Chohan BH, Piantadosi A, Overbaugh J. HIV-1 superinfection and its implications for vaccine design. Curr HIV Res. 2010;8:596–601.

2. Smith DM, Wong JK, Hightower GK, et al.. Incidence of HIV superinfection following primary infection. JAMA. 2004;292:1177–1178.

3. Chohan B, Lavreys L, Rainwater SM, et al.. Evidence for frequent reinfection with human immunodeficiency virus type 1 of a different subtype. J Virol. 2005;79:10701–10708.

4. Piantadosi A, Chohan B, Chohan V, et al.. Chronic HIV-1 infection frequently fails to protect against superinfection. PLoS Pathog. 2007;3:e177.

5. Redd AD, Mullis CE, Serwadda D, et al.. The rates of HIV superinfection and primary HIV incidence in a general population in Rakai, Uganda. J Infect Dis. 2012;206:267–274.

6. Blish CA, Dogan OC, Derby NR, et al.. Human immunodeficiency virus type 1 superinfection occurs despite relatively robust neutralizing antibody responses. J Virol. 2008;82:12094–12103.

7. Brenner BG, Gryllis C, Wainberg MA. Role of antibody-dependent cellular cytotoxicity and lymphokine-activated killer cells in AIDS and related diseases. J Leukoc Biol. 1991;50:628–640.

8. Blish CA, Dogan OC, Jaoko W, et al.. Cellular immune responses and susceptibility to HIV-1 superinfection: a case-control study. AIDS. 2012;26:643–646.

9. Hessell AJ, Hangartner L, Hunter M, et al.. Fc receptor but not complement binding is important in antibody protection against HIV. Nature. 2007;449:101–104.

10. Forthal DN, Gilbert PB, Landucci G, et al.. Recombinant gp120 vaccine-induced antibodies inhibit clinical strains of HIV-1 in the presence of Fc receptor-bearing effector cells and correlate inversely with HIV infection rate. J Immunol. 2007;178:6596–6603.

11. Rainwater S, DeVange S, Sagar M, et al.. No evidence for rapid subtype C spread within an epidemic in which multiple subtypes and intersubtype recombinants circulate. AIDS Res Hum Retroviruses. 2005;21:1060–1065.

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13. Forthal DN, Landucci G, Daar ES. Antibody from patients with acute human immunodeficiency virus (HIV) infection inhibits primary strains of HIV type 1 in the presence of natural-killer effector cells. J Virol. 2001;75:6953–6961.

14. Ahmad A, Morisset R, Thomas R, et al.. Evidence for a defect of antibody-dependent cellular cytotoxic (ADCC) effector function and anti-HIV gp120/41-specific ADCC-mediating antibody titres in HIV-infected individuals. J Acquir Immune Defic Syndr. 1994;7:428–437.

15. Forthal DN, Landucci G, Haubrich R, et al.. Antibody-dependent cellular cytotoxicity independently predicts survival in severely immunocompromised human immunodeficiency virus-infected patients. J Infect Dis. 1999;180:1338–1341.

16. Forthal DN, Landucci G, Keenan B. Relationship between antibody-dependent cellular cytotoxicity, plasma HIV type 1 RNA, and CD4+ lymphocyte count. AIDS Res Hum Retroviruses. 2001;17:553–561.

17. Altfeld M, Allen TM, Yu XG, et al.. HIV-1 superinfection despite broad CD8+ T-cell responses containing replication of the primary virus. Nature. 2002;420:434–439.

18. Basu D, Kraft CS, Murphy MK, et al.. HIV-1 subtype C superinfected individuals mount low autologous neutralizing antibody responses prior to intrasubtype superinfection. Retrovirology. 2012;9:76.

19. Smith DM, Strain MC, Frost SD, et al.. Lack of neutralizing antibody response to HIV-1 predisposes to superinfection. Virology. 2006;355:1–5.

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This article has been cited 1 time(s).

Plos Pathogens
HIV-1 Superinfection Occurs Less Frequently Than Initial Infection in a Cohort of High-Risk Kenyan Women
Ronen, K; McCoy, CO; Matsen, FA; Boyd, DF; Emery, S; Odem-Davis, K; Jaoko, W; Mandaliya, K; McClelland, RS; Richardson, BA; Overbaugh, J
Plos Pathogens, 9(8): -.
ARTN e1003593
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antibody-dependent cell-mediated virus inhibition; ADCVI; HIV-1; superinfection; primary infection; clade A

© 2013 Lippincott Williams & Wilkins, Inc.


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