JAIDS Journal of Acquired Immune Deficiency Syndromes:
Critical Review: Clinical Science
State of The Science of Adherence in Pre-Exposure Prophylaxis and Microbicide Trials
Muchomba, Felix M. MPH*,†; Gearing, Robin E. PhD‡; Simoni, Jane M. PhD§; El-Bassel, Nabila PhD*,†
*Global Health Research Center of Central Asia, Columbia University, New York, NY
†Social Intervention Group, Columbia University, School of Social Work, Columbia University, New York, NY
‡Columbia University, New York, NY
§Department of Psychology, University of Washington, Seattle, WA.
Correspondence to: Felix M. Muchomba, MPH, Global Health Research Center of Central Asia, Columbia University, 1255 Amsterdam Avenue, New York, NY 10027 (e-mail: email@example.com).
J.M. Simoni is supported by the National Institute to Mental Health (K24MH093243) and the University of Washington Center for AIDS Research (CFAR), a National Institutes of Health–funded program (P30 AI27757). N. El-Bassel is supported by the National Institute of Drug Abuse (R01DA022914).
The authors have no conflicts of interest to disclose.
Received May 7, 2012
Accepted August 16, 2012
Abstract: For pre-exposure prophylaxis (PrEP) and microbicides to effectively prevent HIV, optimal treatment adherence is required. Adherence to these strategies, however, has not been sufficiently studied. This investigation systematically reviews oral PrEP and microbicide trials across 4 domains of adherence: (1) definition and measures used, (2) risks for nonadherence, (3) promotion strategies, and (4) effects on outcomes. Nineteen (n = 19) trials, with 47,157 participants, published between 1987 and 2012 were identified. Reported mean adherence to microbicides was 79% and to oral PrEP 87%. Common risks for microbicide nonadherence were decreased motivation over time, sex with primary (noncommercial/casual) partners, and insufficient supply. Oral PrEP nonadherence risks were older age and medication side effects. Psychoeducation and outreach to participants and communities were frequently used promotion strategies. Most trials failed to systematically identify barriers and monitor and promote adherence, although adherence moderated outcomes. Recommendations for attending to adherence in future trials are provided.
Significant strides in HIV prevention have been achieved through the use of active pharmaceutical agents with individuals at risk but currently HIV-negative.1 These agents include antiretroviral treatments administered orally [ie, oral pre-exposure prophylaxis (PrEP)]2,3 and antimicrobial gels, creams, and films applied topically (ie, microbicides).4,5 The effectiveness of PrEP in reducing incidence of HIV is strongly supported, and the evidence on the use of microbicides is promising.6
While these interventions are typically referred to as biomedical prevention strategies, in reality, they are better characterized as bio-behavioral because of the significant behavioral factors that seem to impact their implementation and moderate their efficacy.7 Perhaps most importantly, individual adherence to the prevention protocols seems paramount to optimal response, yet may be difficult to achieve in those who are currently HIV-negative.8,9 Despite advances in adherence research and strategies in antiretroviral therapy,10 adherence to these bio-behavioral prevention strategies (B-BPSs) remains underdeveloped and underresearched. Adherence barriers encountered in antiretroviral therapy, such as depression,11–13 substance use,11–13 inadequate patient–provider relationships,12,14 lack of social support,12,15 negative publicity,12 stigma,16,17 migration,16 poverty,18 inconvenient dosing frequency,14 distance to health facility,17 lack of coordination across health services,17 and limited involvement of community in program planning,17 may impede adherence to B-BPSs. Investigation of adherence barriers and strategies to promote adherence is therefore required, for which effective adherence measurement is essential.19
This systematic review examines: (1) definitions and measures used for adherence in oral PrEP and microbicide research; (2) the individual-, partner-, agency-, community-, or structural-level risks for nonadherence; (3) strategies/interventions used to promote adherence; and (4) effects of adherence on the major outcomes. Based on the review, recommendations for future research and implementation of B-BPSs are offered.
Empirical studies on oral PrEP and topical microbicides taken prophylactically by HIV-negative individuals to prevent HIV infection were searched using Medline, PsycINFO, and Cochrane Library databases. Searches were limited to clinical randomized controlled trials published in English between January 1, 1987 and January 1, 2012 that matched the MeSH keywords: HIV infections, anti-infective agents, vaginal creams, foams, and jellies, or gels, and any of the following terms: negative, seronegative, uninfected, pre-exposure, microbicide, prophylaxis, cellulose sulfate, tenofovir, carrageenan, naphthalenesulfonates, truvada, buffergel, PRO2000, or adenine. The comprehensiveness of search results was further verified by reviewing cited references, conference abstracts databases (Conference on Retroviruses and Opportunistic Infections Abstracts, International Aids Conference Abstracts, and International Aids Society Conference on HIV Pathogenesis and Treatment and Prevention Abstracts), and HIV prevention trials Web sites (HIV Prevention Trials Network, Microbicide Trials Network, Global Advocacy for HIV Prevention, and the Centers for Disease Control and Prevention). Studies were excluded when: (1) efficacy or effectiveness in preventing HIV infection was not assessed, (2) neither preliminary nor final outcomes were reported, (3) the treatment was a vaccine, or (4) the intervention being assessed was treatment of STIs other than HIV. The search identified 591 articles. A review of the abstracts by the research team resulted in the inclusion of 33 articles representing 19 randomized controlled trials.
For each eligible study, authors used a comprehensive template to extract features of the study population, preventive treatment, treatment protocol, study findings, and descriptions of adherence measurement, effect of adherence on outcomes, risks for nonadherence, and strategies used to improve adherence. Other articles about the same study were identified and reviewed for additional details or verification of extracted data. The lead researcher in one was contacted for and provided additional details (J. Baeten, MD, PhD, written communication, February 2012).
Two authors (F.M.M. and R.E.G.) separately identified risks and strategies and coded them as individual-, partner-, agency-, or community-level based on whether they were focused on the study participants, the sexual partners of study participants, study staff and structure, or leaders and members of the communities to which the participants belonged respectively. Nonadherence risks that involved both the study participants and their partners (ie, lack of time to apply microbicide and sex acts with primary partner) were coded as both individual- and partner-level risks. Individual-, partner-, and community-level strategies were further grouped into psychoeducation,20 outreach (ie, contacting participants who missed follow-up visits or to remind them to take treatment), and dosing (ie, use of schedules and dose organizing tools). Agency-level strategies were further categorized into staff training, client-provider relations, and convenient locations and hours of operation. Interrater agreement surpassed 90%, with all differences resolved through discussion.
As seen in Table 1, the number of studies increased over time, with 2 studies conducted before 2000,33,34 3 studies between 2000 and 2005,28,29,31 and 14 studies after 2005.21–27,30,32,35,37–40 Most (n = 14) studies were microbicide studies, and only 1 study had both microbicide and oral PrEP treatment arms.21–34
TABLE 1-a Characteri...Image Tools
TABLE 1-b Characteri...Image Tools
TABLE 1-c Characteri...Image Tools
A total of 47,157 participants were investigated across the 19 studies (Table 1). The range in sample size was larger in microbicide studies (138–9385) than in PrEP studies (936–5029) and mean sample size in microbicide studies was smaller (2557 vs. 2732).
All participants in microbicide studies were females at risk for HIV because of sexual activity,21–34 with 6 specifying sex workers.27,28,31–34 Six (43%) microbicide studies excluded injection drug users,22,24,27,30–32 1 study reported levels of drug (khat) use,28 and the remainder did not report drug use characteristics of the participants.
Participants in oral PrEP studies also varied. Two studies included only female participants, of which, 1 recruited sex workers.37,39 Of the 2 PrEP studies that had both female and male participants, 1 recruited HIV-serodiscordant couples.35,40 The remaining oral PrEP study recruited men and transgender women who had sex with men, with some participants reporting engaging in sex work.38 Alcohol use was reported in 1 oral PrEP study.38 No other studies reported drug or alcohol use among participants.
At 12 months, 5 (26%) studies reported a retention rate of 70% or less.23,24,28,31,39 On average, studies that reported recruiting sex workers had lower retention rates (73%) than those that did not (87%). The most often reported reason for participants' discontinuation was pregnancy.23–25,27,30–32,34,35,39 Studies did not report whether participants who dropped out were less adherent than retained participants before leaving the study.
Treatment Characteristics and Outcomes
One microbicide study used a sponge,33 another used a film,34 and the rest used gels.21–32 Most (n = 10) microbicide studies required treatment application before every sex act. Oral PrEP studies largely used an Emtricitabine and Tenefovir Disoproxil Fumarate combination drug.26,35,37,38,40
One microbicide treatment arm and 4 oral PrEP treatment arms were found to have a statistically significant protective effect.21,35,38,40 Eight microbicide and 3 oral PrEP treatment arms across 9 studies were terminated prematurely.23–28,32,37,39
Twelve studies reported 1 method of adherence measurement, 1 study reported 2, and 6 studies reported more than 2. Eight studies (42%) measured adherence solely through self-report,22–24,27–29,31,34 1 microbicide study used an applicator staining assay to test for vaginal insertion,30 and 2 oral PrEP studies used blood level tests.37,38 Most (89%) studies assessed adherence once a month. Two studies assessed adherence quarterly.22,30 Of the 12 microbicide studies that included self-report adherence measures, 4 used a 7-day recall period,23,24,27,29 2 asked about microbicide use at the last sex act,25,30 2 used a measure with a 7-day recall period and an additional measure regarding the last sex act or last working day for sex workers,22,31 1 used a 1-month recall period,21 and 3 did not report information on the self-report measures used.26,28,34 Both oral PrEP studies that included self-report measures used a 4-week recall period.37,38 Studies did not report psychometric properties of the self-report measures.
Self-report measures were biased upward in the 2 studies that reported adherence rates from multiple measures.30,38 Adherence based on self-reports was much higher than adherence based on applicator staining assays (96% vs. 41%) in one of the studies and slightly higher than that based on pill counts (95% vs. 86%) and prescription refills (95% vs. 91%) in the second study.
Adherence to Microbicides
Three microbicide studies reported adherence rates in terms of proportion of participants correctly adhering to treatment protocols.22,25,30 Adherence rates reported in the remaining studies referred to average proportion of sexual acts in which microbicides were used. Adherence rates in microbicide studies ranged from 69% to 89% with a study mean of 79%. The average adherence rate for microbicides that required application before every sex act was higher (80%) than for microbicides that required only once or twice daily application (76%). The adherence rate was comparable between microbicide studies that reported including sex workers versus those that did not (81% vs. 78%); however, no study reported whether sex workers had a different adherence level from non–sex workers.
Adherence to Oral PrEP
Adherence rates reported in oral PrEP studies referred to average proportion of pills taken. Adherence rates in oral PrEP studies ranged from 69% to 97%, with a study mean of 87%, indicating higher adherence rates to oral PrEP than to microbicides. The adherence rate was lower in oral PrEP studies that reported including sex workers versus those that did not (80% vs. 92%).
Impact of Adherence on Studies' Outcomes
One oral PrEP study and 3 microbicide studies analyzed and reported the impact of adherence on efficacy.21,25,30,38 High adherers, identified on the basis of pill counts, self-report, and dispensation records, achieved 41% higher efficacy compared with low adherers in the oral PrEP study. In a microbicide study that also reported a statistically significant treatment effect, high adherers, identified by comparing gel applicator returns and self-reported frequency of sexual intercourse, had a 26% higher efficacy. High adherers had a 20% higher efficacy, based on self report, and 16% lower efficacy, based on comparisons between gel applicator testing and self-reported frequency of sexual intercourse, in the remaining 2 microbicide studies that did not report statistically significant treatment effects.
Risks for Microbicide Nonadherence
Few risks for nonadherence were reported in the studies included in this review (Fig. 1). On average, 1 risk was reported in each study (SD = 2.3). Individual-level risks to microbicide adherence identified were (1) heightened frequency of sex acts,21 (2) decreased motivation to use microbicides over time,23,24,27 (3) forgetting to use the microbicide,30 (4) increased number of sex acts with primary partners,32,34 (5) lack of time to apply microbicide or to replenish supplies,32 and (6) perceptions and beliefs such as fear of detection by partner, worry about potential side effects, concern that gel would accumulate from multiple applications within a short time, and a belief that gel use in sex acts occurring during menses is detrimental.32 Partner-level risks for microbicide nonadherence were (1) sex acts with primary partner,32,34 (2) lack of sexual partner cooperation,32 and (3) lack of time to apply microbicide while commercial sex client waited.32 Insufficient supply was the only agency-level risk for microbicide nonadherence.30,32 The most often cited risk for microbicide nonadherence was decrease in motivation to use the treatment over time followed by sex with primary partners and running out of treatment drug.
Risks for Oral PrEP Nonadherence
Most (n = 4) oral PrEP studies did not report nonadherence risks. Older age and medication side effects were the only risks for oral PrEP nonadherence noted.36,38
Adherence Intervention Strategies Used
On average, 3.3 adherence intervention strategies were identified in each study. Individual-level strategies were the most common and included general psychoeducation, skills building, a personalized plan for adherence, adapting dosing protocols to participants' schedules, using calendars and pill organizers, outreach to participants who missed a visit, and proactive outreach using cell phone alarms to remind participants to follow treatment protocols. Partner-level strategies involved either initial or ongoing psychoeducation intervention with the partner such as delivering study information to participants and their male partners as a couple. Agency level strategies included having convenient locations and hours of operation, positive client–provider relationships, and training staff and counselors. Community-level strategies included using community forums and boards to provide information about the study and to elicit feedback. Individual-level strategies were used by all studies. Two studies used partner-level strategies,22,25 6 studies used agency-level strategies,21,22,24,29,32,37 and 6 studies used community-level strategies.21,22,25,34,37,38 Most intervention strategies were delivered by agency staff. In the study with discordant couples,35 anecdotal evidence suggests that support from the HIV-infected partner, particularly the taking of medication together, reinforced adherence to oral PrEP (J. Baeten, MD, PhD, written communication, February 2012).
This systematic review of the state of the science of adherence research in PrEP and microbicide studies found that few studies included detailed information on adherence. In those that did, adherence was associated with higher efficacy. Clearly, a lack of vigilance to adherence issues in trials may undermine estimates of the efficacy of these important B-BPSs. This review follows and builds on ongoing, but yet to be published, efforts of B-BPS researchers to promote adherence.
Assessing adherence is essential not only to determining accurate estimates of prevention efficacy but also for whom and in what circumstances adherence is problematic. As there is no “gold standard” for measuring adherence, multiple methods will likely be required.19 Studies that assessed adherence using multiple measures found self-report measures overestimated adherence. This is consistent with the adherence literature, which indicates that self-report is subject to overreporting bias particularly if individuals perceived negative consequences to admitting nonadherence.41 However, the majority of the studies reviewed in this paper used only one measure to assess adherence, many relying solely on self-report.
The most identified risks for nonadherence in microbicide research were a decline in motivation to use the product over time, sex with primary as opposed to casual or commercial partners, and insufficient supply. Risks for oral PrEP nonadherence cited were older age and medication side effects. Intervention strategies used to promote adherence to microbicides and oral PrEP were individual-, partner-, and community-level psychoeducation, adaptation of dosing protocols into participants' schedules, use of organizing tools, outreach to participants and communities, convenient locations and hours of operation, positive client–provider relationships, and staff training.
Adherence in microbicide studies was, on average, lower than in oral PrEP studies. Microbicide studies noted that sex acts with primary rather than casual or commercial partners, fear of detection of microbicide by partners, and lack of cooperation from partners hindered adherence, indicating that primary partner involvement might be important to promote adherence even when the strategies are women initiated and women controlled. Vulnerable populations like sex workers and drug users may face unique lifestyle and social circumstances that undermine adherence to the treatment, but studies generally failed to report this information or simply excluded vulnerable groups.22,24,27,30–32
Study limitations include the inability to geographically generalize findings, as most of the studies were conducted in sub-Saharan Africa. Comprehensiveness of the review may have been compromised by page limits in journals and lack of access to study protocols and manuals. Also, the likelihood of publication bias cannot be ruled out, despite the many nonsignificant findings published.
RECOMMENDATIONS FOR FUTURE TRIALS ON BIO-BEHAVIORAL PREVENTION STRATEGIES (B-BPSs)
Drawn from this review, 5 areas of recommendations for future research on B-BPS are outlined: (1) barriers to adherence, (2) strategies to promote adherence, (3) assessment of adherence and fidelity, (4) retention, and (5) impact of adherence on study outcomes (reduced incidence of HIV).
1. Pretrial formative work should include a multilevel assessment of potential barriers to optimal adherence, especially in any vulnerable populations to be targeted. Stigma toward the use of B-BPSs, mental health conditions, such as depression and substance use, and structural barriers encountered in antiretroviral therapy adherence should be considered.11–18 Development of a risk screen based on known risk factors for nonadherence is also recommended.
2. Trial protocols should incorporate evidence-based and culturally appropriate strategies to systematically promote adherence in all participants.42 Multilevel strategies that engage sexual partners, client–provider relationships, peer networks, communities, and case management can provide social support and address gender, community, and cultural norms regarding use of oral PrEP and microbicides.10,43 Couple-based approaches need to be provided as a choice for women in microbicide studies. Giving women an option to involve their partners may increase partner cooperation and improve adherence.44,45 While microbicides seem to be women initiated and women controlled; women also need education on possible partner responses and how to handle the responses and choice to involve the partner.
3. Trial procedures must include a priori plans to accurately and longitudinally monitor adherence. Measurements that are not subject to reporting bias, such as biomarkers46 and applicator staining assays,47,48 are recommended as one of several measures used for triangulation. As use of self-report measures is likely to continue, attention to the psychometric properties of these measures, including their recall time frame, is needed to improve their validity and reliability. Additionally, research into factors that reduce bias of self-reports such as normalizing nonadherence is recommended. Adherence measurement should be supplemented by assessment of fidelity, defined as the extent to which core components of interventions are delivered as intended by the protocols.49 Failure to implement a study with a high degree of fidelity could undermine a valuable prevention strategy.
4. Development of B-BPS that will not interfere with pregnancy is recommended. Restricting studies to women already on effective contraceptives is a strategy that one study has opted for to address high study discontinuation rates.50
5. Analyses of efficacy should include (a priori) plans to investigate the role of adherence as a key effect moderator.
1. Cohen MS, Gay C, Kashuba AD, et al.. Narrative review: antiretroviral therapy to prevent the sexual transmission of HIV-1. Ann Intern Med. 2007;146:591–601.
2. Garcia-Lerma JG, Paxton L, Kilmarx PH, et al.. Oral pre-exposure prophylaxis for HIV prevention. Trends Pharmacol Sci. 2010;31:74–81.
3. Padian NS, Buve A, Balkus J, et al.. Biomedical interventions to prevent HIV infection: evidence, challenges, and way forward. Lancet. 2008;372:585–599.
4. Balzarini J, Van Damme L. Microbicide drug candidates to prevent HIV infection. Lancet. 2007;369:787–797.
5. McGowan I. Microbicides for HIV prevention: reality or hope? Curr Opin Infect Dis. 2010;23:26–31.
6. Karim SS, Karim QA. Antiretroviral prophylaxis: a defining moment in HIV control. Lancet. 2011;378:e23–e25.
7. Kippax S, Stephenson N. Beyond the distinction between biomedical and social dimensions of HIV prevention through the lens of a social public health. Am J Public Health. 2012;102:789–799.
8. Grant RM. Antiretroviral agents used by HIV-uninfected persons for prevention: pre-and postexposure prophylaxis. Clin Infect Dis. 2010;50(suppl 3):S96–S101.
9. Karim SS, Kashuba AD, Werner L, et al.. Drug concentrations after topical and oral antiretroviral pre-exposure prophylaxis: implications for HIV prevention in women. Lancet. 2011;378:279–281.
10. Simoni JM, Amico KR, Pearson CR, et al.. Strategies for promoting adherence to antiretroviral therapy: a review of the literature. Curr Infect Dis Rep. 2008;10:515–521.
11. DiMatteo MR, Lepper HS, Croghan TW. Depression is a risk factor for noncompliance with medical treatment: meta-analysis of the effects of anxiety and depression on patient adherence. Arch Intern Med. 2000;160:2101–2107.
12. Mills EJ, Nachega JB, Bangsberg DR, et al.. Adherence to HAART: a systematic review of developed and developing nation patient-reported barriers and facilitators. PLoS Med. 2006;3:e438.
13. Mugavero M, Ostermann J, Whetten K, et al.. Barriers to antiretroviral adherence: the importance of depression, abuse, and other traumatic events. AIDS Patient Care STDS. 2006;20:418–428.
14. Chesney MA. Factors affecting adherence to antiretroviral therapy. Clin Infect Dis. 2000;30(suppl 2):S171–S176.
15. Fogarty L, Roter D, Larson S, et al.. Patient adherence to HIV medication regimens: a review of published and abstract reports. Patient Educ Couns. 2002;46:93–108.
16. Weiser S, Wolfe W, Bangsberg D, et al.. Barriers to antiretroviral adherence for patients living with HIV infection and AIDS in Botswana. J Acquir Immune Defic Syndr. 2003;34:281.
17. Posse M, Meheus F, Van Asten H, et al.. Barriers to access to antiretroviral treatment in developing countries: a review. Trop Med Int Health. 2008;13:904–913.
18. Kagee A, Remien R, Berkman A, et al.. Structural barriers to ART adherence in Southern Africa: challenges and potential ways forward. Glob Public Health. 2011;6:83–97.
19. Williams AB, Amico KR, Bova C, et al.. A proposal for quality standards for measuring medication adherence in research. AIDS Behav. 2012:1–14. [epub ahead of print; DOI: 10.1007/s10461-012-0172-7].
20. Lukens EP, McFarlane WR. Psychoeducation as evidence-based practice: considerations for practice, research, and policy. Brief Treat Crisis Interv. 2004;4:205.
21. Abdool Karim Q, Abdool Karim SS, Frohlich JA, et al.. Effectiveness and safety of tenofovir gel, an antiretroviral microbicide, for the prevention of HIV infection in women. Science. 2010;329:1168–1174.
22. Abdool Karim SS, Richardson BA, Ramjee G, et al.. Safety and effectiveness of BufferGel and 0.5% PRO2000 gel for the prevention of HIV infection in women. AIDS. 2011;25:957.
23. Feldblum PJ, Adeiga A, Bakare R, et al.. SAVVY vaginal gel (C31G) for prevention of HIV infection: a randomized controlled trial in Nigeria. PLoS One. 2008;3:e1474.
24. Halpern V, Ogunsola F, Obunge O, et al.. Effectiveness of cellulose sulfate vaginal gel for the prevention of HIV infection: results of a phase III trial in Nigeria. PLoS One. 2008;3:e3784.
25. McCormack S, Ramjee G, Kamali A, et al.. PRO2000 vaginal gel for prevention of HIV-1 infection (Microbicides Development Programme 301): a phase 3, randomised, double-blind, parallel-group trial. Lancet. 2010;376:1329–1337.
26. Microbicide Trials Network (MTN). Microbicide trials network statement on decision to discontinue use of tenofovir gel in VOICE, a major HIV prevention study in women. Microbicide Trials Network Web site. Available at: http://www.mtnstopshiv.org/node/3909
. Accessed December 17, 2011.
27. Peterson L, Nanda K, Opoku BK, et al.. SAVVY®(C31G) gel for prevention of HIV infection in women: a phase 3, double-blind, randomized, placebo-controlled trial in Ghana. PLoS One. 2007;2:e1312.
28. Richardson BA, Lavreys L, Martin HL Jr, et al.. Evaluation of a low-dose nonoxynol-9 gel for the prevention of sexually transmitted diseases: a randomized clinical trial. Sex Transm Dis. 2001;28:394.
29. Roddy RE, Zekeng L, Ryan KA, et al.. Effect of nonoxynol-9 gel on urogenital gonorrhea and chlamydial infection. JAMA. 2002;287:1117–1122.
30. Skoler-Karpoff S, Ramjee G, Ahmed K, et al.. Efficacy of Carraguard for prevention of HIV infection in women in South Africa: a randomised, double-blind, placebo-controlled trial. Lancet. 2008;372:1977–1987.
31. Van Damme L, Ramjee G, Alary M, et al.. Effectiveness of COL-1492, a nonoxynol-9 vaginal gel, on HIV-1 transmission in female sex workers: a randomised controlled trial. Lancet. 2002;360:971–977.
32. Van Damme L, Govinden R, Mirembe FM, et al.. Lack of effectiveness of cellulose sulfate gel for the prevention of vaginal HIV transmission. N Engl J Med. 2008;359:463–472.
33. Kreiss J, Ngugi E, Holmes K, et al.. Efficacy of nonoxynol 9 contraceptive sponge use in preventing heterosexual acquisition of HIV in Nairobi prostitutes. JAMA. 1992;268:477–482.
34. Roddy RE, Zekeng L, Ryan KA, et al.. A controlled trial of nonoxynol 9 film to reduce male-to-female transmission of sexually transmitted diseases. N Engl J Med. 1998;339:504–510.
35. Baeten J, Celum C. Antiretroviral pre-exposure prophylaxis for HIV-1 prevention among heterosexual African men and women: the Partners PrEP Study. The 6th IAS Conference on HIV Pathogenesis, Treatment and Prevention, Rome, Italy, July 17–20, 2011. Abstract MOAX0106.
36. Haberer J, Baeten J, Celum C, et al.. Near perfect early adherence to antiretroviral pre-exposure prophylaxis (PrEP) against HIV infection among HIV-serodiscordant couples as determined by multiple measures: preliminary data from the partners PrEP study. 18th Conference on Retroviruses and Opportunistic Infections, Boston, MA, February 27–March 2, 2011. Paper 488.
38. Grant RM, Lama JR, Anderson PL, et al.. Preexposure chemoprophylaxis for HIV prevention in men who have sex with men. N Engl J Med. 2010;363:2587–2599.
39. Peterson L, Taylor D, Roddy R, et al.. Tenofovir disoproxil fumarate for prevention of HIV infection in women: a phase 2, double-blind, randomized, placebo-controlled trial. PLoS Clin Trials. 2007;2:e27.
40. Thigpen M, Kebaabetswe P, Smith D, et al.. Daily oral antiretroviral use for the prevention of HIV infection in heterosexually active young adults in Botswana: results from the TDF2 study. The 6th IAS Conference on HIV Pathogenesis, Treatment and Prevention, Rome, Italy, July 17–20, 2011. Abstract WELBC01.
41. Wilson IB, Carter AE, Berg KM. Improving the self-report of HIV antiretroviral medication adherence: is the glass half full or half empty? Curr HIV/AIDS Rep. 2009;6:177–186.
42. Gearing RE, Townsend L, MacKenzie M, et al.. Reconceptualizing medication adherence: six phases of dynamic adherence. Harv Rev Psychiatry. 2011;19:177–189.
43. Ziguras SJ, Stuart GW. A meta-analysis of the effectiveness of mental health case management over 20 years. Psychiatr Serv. 2000;51:1410–1421.
44. El-Bassel N, Gilbert L, Witte S, et al.. Couple-based HIV prevention in the United States: advantages, gaps, and future directions. J Acquir Immune Defic Syndr. 2010;55:S98.
45. Martire LM, Schulz R, Helgeson VS, et al.. Review and meta-analysis of couple-oriented interventions for chronic illness. Ann Behav Med. 2010;40:325–342.
46. Atkinson AJ, Colburn WA, DeGruttola VG, et al.. Biomarkers and surrogate endpoints: preferred definitions and conceptual framework. Clin Pharmacol Ther. 2001;69:89–95.
47. Wallace A, Thorn M, Maguire RA, et al.. Assay for establishing whether microbicide applicators have been exposed to the vagina. Sex Transm Dis. 2004;31:465.
48. Wallace AR, Teitelbaum A, Wan L, et al.. Determining the feasibility of utilizing the microbicide applicator compliance assay for use in clinical trials. Contraception. 2007;76:53–56.
49. Gearing RE, El-Bassel N, Ghesquiere A, et al.. Major ingredients of fidelity: a review and scientific guide to improving quality of intervention research implementation. Clin Psychol Rev. 2011;31:79–88.
51. Family Health International, The Centre for the AIDS Programme of Research in South Africa. Factsheet: CAPRISA 004 and adherence to study product. Research Triangle Park, NC USA: FHI and the Centre for the AIDS Programme of Research in South Africa; 2010:4.
52. Seyama L. Community engagement in HPTN 035: MTN annual meeting, April 21, 2009, Arlington, Virginia USA. MTN 2009 Annual Meeting Presentations Web site. Available at: http://www.mtnstopshiv.org/2009annualmeeting
. Accessed February 1, 2012.
53. Pool R, Montgomery CM, Morar NS, et al.. A mixed methods and triangulation model for increasing the accuracy of adherence and sexual behaviour data: The microbicides development programme. PloS one. 2010;5:e11600.
54. Family Health International. Technical report: Behavioral and social science support to CONRAD phase III clinical trial of cellulose sulfate 6% gel. Research Triangle Park, NC USA: FHI 360; 2008:89.
adherence; pre-exposure prophylaxis; microbicides; review
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