Aluisio, Adam MS*; Richardson, Barbra A PhD, MS†‡; Bosire, Rose MBChB, MPH§; John-Stewart, Grace MD, PhD‖¶#; Mbori-Ngacha, Dorothy MBChB, MMed, MPH**; Farquhar, Carey MD, MPH‖¶#
From the *Stony Brook University Medical Center, Stony Brook, NY; †Department of Biostatistics, University of Washington, Seattle, WA; ‡Division of Public Health Sciences, Fred Hutchinson Cancer Research Cancer, Seattle, WA; §Kenya Medical Research Institute, Nairobi, Kenya; Departments of ∥Epidemiology; ¶Medicine; and #Global Health, University of Washington, Seattle, WA; and **Department of Paediatrics, University of Nairobi, Nairobi, Kenya.
Received for publication July 9, 2010; accepted September 21, 2010.
Supported by grant HD-23412 from the US National Institute of Child Health and Human Development.
A. Aluisio was a scholar in the International AIDS Research and Training Program and was supported by grant 5 R24 TW007988 from the National Institutes of Health, Fogarty International Center through Vanderbilt University.
Presented orally at the 5th IAS Conference on HIV Pathogenesis, Treatment and Prevention, July 19-22, 2009, Cape Town, South Africa.
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
The authors have no conflicts of interest to disclose.
Correspondence to: Adam Aluisio, MS, University of Washington, 325 Ninth Avenue, Box 359909, Seattle, WA 98104 (e-mail: firstname.lastname@example.org).
An estimated 1000 children are infected with HIV type 1 daily, 90% of whom live in sub-Saharan Africa. Vertical transmission accounts for approximately 95% of infections in children.1,2 Despite improved access to antiretrovirals for prevention of mother-to-child transmission (PMTCT) of HIV, utilization in resource-limited settings is suboptimal with more than one third of HIV-infected pregnant women and half of their infants failing to receive medications.3,4 High HIV transmission rates, infant feeding practices, and poverty contribute to infant mortality in sub-Saharan Africa, where rates are among the highest in the world.5-7 The extent and interplay of these public health problems makes addressing them in concert of integral importance to improving infant health.
One factor that may be associated with vertical transmission and infant mortality is male partner involvement. Although few data exist on the relationship between male involvement and infant mortality, the role of partners in PMTCT utilization has been well documented.8-12 Male involvement in antenatal voluntary counseling and testing has been associated with increased use of antiretroviral prophylaxis in their HIV-infected pregnant partners.9,12 Furthermore, improved adherence to PMTCT feeding strategies has been associated with partner support.8,11,12 Although there is considerable evidence that male involvement improves PMTCT utilization, limited data exist supporting an association between that involvement and vertical transmission or infant mortality, and this was our goal in the analysis of this prospective cohort.
SUBJECTS AND METHODS
Recruitment and Clinical Procedures
HIV-positive pregnant women were recruited from antenatal clinics in Nairobi, Kenya, from 1999 to 2002 and followed up until 2005 in a prospective cohort study, as previously described.13-15 Recruited women were referred to the study clinic and encouraged to invite their partners for participation. After giving written informed consent, women were enrolled and followed from 32-week gestation until delivery and seen monthly postpartum with their infants for 12 months. At enrollment a standardized questionnaire was administered accessing maternal health and sociodemographic and partnership characteristics. The partnership assessment ascertained if the male partner of the enrolled women had been previously tested for HIV; however, it did not identify the type or results of previous testing.
Women received short-course zidovudine from 34- to 36-week gestation through delivery per Kenyan national guidelines at the time. Participants delivered at the Kenyatta National Hospital, where study nurse midwives collected intrapartum and neonatal specimens. Infants were evaluated within 48 hours of delivery, and during 12 monthly follow-up visits. Maternal blood samples for HIV RNA levels and CD4+ cell counts were obtained at enrollment. Infant blood specimens were collected on filter papers within 48 hours of birth and at 1, 3, 6, 9, and 12 months postpartum for HIV DNA testing.
Male partners were enrolled at the antenatal study clinic after obtaining written informed consent. Enrolled partners received counseling on vertical transmission and prevention methods from trained study personnel in the antenatal clinic, and a questionnaire was administered assessing sociodemographics and sexual and medical histories. Men were offered voluntary counseling and testing in the antenatal setting and those who accepted were asked to return after 1 week for results and posttest counseling.
HIV testing was performed using a commercially available enzyme-linked immunosorbent assay (DuPont, Geneva, Switzerland). Maternal plasma HIV RNA viral load was quantified using a transcription-mediated amplification method (GenProbe, San Diego, CA) sensitive for HIV subtypes common in Kenya.16 Polymerase chain reaction was used to determine infant HIV infection status by detecting HIV DNA gag sequences in blood specimens collected on filter papers.17 Infant plasma HIV RNA quantification was conducted to confirm timing of infection.16 Infants were considered HIV infected if they had 2 consecutive positive HIV DNA polymerase chain reaction tests or if the final visit sample was positive.
Data analysis was performed using STATA version 11.0 (StataCorp LP, College Station, TX). Analyses were restricted to mother-infant pairs with follow-up through delivery and to women who reported having a current male partner. Correlates of male attendance were compared using independent sample t tests for continuous variables, Pearson χ2 test for categorical variables, and logistic regression for multivariate testing.
The role of male involvement (represented by antenatal attendance and previous HIV testing as per the report by women) in infant outcomes of HIV infection and mortality were examined using incidence rates and regression models. Additionally, Kaplan-Meier analyses were performed with differences in time to events tested using log rank tests. In regression models, outcomes were analyzed separately and as a combined end point (HIV infection or mortality). Cox proportional hazards models were undertaken to assess the effects of male partner involvement. Multivariate models adjusting for log10 maternal viral load at enrollment and infant feeding (formula versus breastfeeding) were used to determine the effects of partner involvement independent of these risk factors. Infants were classified as breastfed if mothers reported any breastfeeding during the first year of life. To avoid colinearity and assess the effect of each factor independently, male antenatal attendance and previous HIV testing were analyzed in separate models.
In vertical transmission analyses for infants infected between 48 hours and 12 months postpartum, the midpoint between the last HIV-negative and first HIV-positive result was used as the time of event. Time of infection for infants testing HIV-positive before 48 hours postpartum was defined as immediately after birth. HIV-uninfected infants were censored at time of death, last clinic visit, or 1 year postpartum.
In mortality analyses, time of infant death was based on clinic records and maternal report, and event time for stillborn infants was set as immediately postpartum. Infant HIV infection was found to modify the effect of partner involvement on mortality; therefore, separate models based on infant infection status were used. In models of mortality among HIV-uninfected infants, HIV-infected infants were censored at the time of infection. In models of mortality among HIV-infected infants, entry into the models was set at the time of infection. Live infants were censored at their last clinic visit or at 1 year postpartum.
For HIV-free survival representing the combined risk of HIV infection or infant mortality, timing of events was first based on HIV infection and subsequently on mortality as described above. Live HIV-uninfected infants were censored at their last clinic visit or at 1 year postpartum.
Study Population and Follow-up
Between July 1999 and October 2002, 510 HIV-infected pregnant women were enrolled, and of those 27 (5%) were lost to follow-up before delivery and 27 (5%) were excluded from the analysis because they reported no current male partner relationship. Of the 456 remaining women, 140 women (31%) were accompanied by their male partners and 316 (69%) reported having a partner (steady boyfriend or husband) who failed to attend (Fig. 1).
For the 140 male partners who attended, median years of education were 9.5 [interquartile range (IQR) 8-12] and 96% reported a means of income. Self-reported history of a sexually transmitted infection was 13%. Seventy-five men (54%) were tested for HIV in the antenatal clinic, and 42 (56%) tested positive (Table 1).
Eight-four percent of women reported being in a monogamous marriage with no difference in relationship classification found between reports by male and female participants. Sixty-six percent of women received >3 weeks of zidovudine, 16% delivered by cesarean, and 32% intended to formula feed at enrollment. Seventy-eight percent of women reported that they had disclosed their serostatus to their partners, and 49% reported discussing PMTCT with them. Previous male partner HIV-testing was reported by 52% of female participants.
Of the 456 infants, 392 (86%) were followed until 12 months postpartum or death. Loss to follow-up was not significantly different between infants born to women with male antenatal attendance (20 infants [14%]) and those born to women without (44 infants [14%]) (Pearson χ2 test, P = 0.92). Three hundred fourteen (69%) infants were breastfed, and the median duration of feeding was 6 months (IQR 3-8). Eighty-two (19%) of 441 infants tested acquired HIV, with 27 (33%) testing HIV DNA positive by 48 hours postpartum. Seventy-one infants (16%) died, 28 (39%) of whom were HIV infected, 31 (44%) were HIV uninfected, and 12 (17%) had unknown serostatus.
Correlates of Male Partner Attendance
Associations were found between male attendance and relationship status, report of PMTCT partner discussions, and prior HIV testing (Table 2). Women whose partners attended clinic were more likely to be in a monogamous marriage [odds ratio (OR) = 2.11; 95% confidence interval (CI): 1.14 to 3.92; P = 0.018], to report previous male partner HIV testing (OR = 25.86; 95% CI: 13.48 to 49.62; P < 0.001), and to have discussed PMTCT with their partners (OR = 3.84; 95% CI: 2.35 to 6.25; P < 0.001) (Table 2). In multivariate analysis, only previous male HIV testing remained significantly associated with male partner antenatal attendance [adjusted odds ratio (aOR) = 24.48; 95% CI: 11.49 to 52.14; P < 0.001]. Duration of relationship, personal income, and maternal viral load were not significantly different for women with and without partner attendance (Table 2) only previously male testing remained significantly associated with male antenatal.
PMTCT Interventions and Male Involvement
Any use of zidovudine and zidovudine adherence for >3 weeks were not significantly different between women with and without male antenatal attendance. Previous male HIV testing was associated with a trend toward increased zidovudine adherence among their HIV-infected pregnant partners (OR = 1.48; 95% CI: 0.96 to 2.29; P = 0.08). Women reporting previous male HIV testing were more likely to formula feed their infants (OR = 1.59; 95% CI: 1.02 to 2.44; P = 0.037), as were women who reported discussing formula feeding with their partners (OR = 3.69; 95% CI: 2.42 to 5.63; P < 0.001).
Infant Health Outcomes
Among infants born to women with male attendance, incident HIV infection was 16.30 per 100 person-years (95% CI: 10.13 to 26.22) versus 30.86 per 100 person-years (95% CI: 24.20 to 39.35) among those born to mothers without partner attendance. For children born to women reporting previous male HIV testing, HIV incidence was 18.30 per 100 person-years (95% CI: 12.55 to 26.68) compared with 35.13 per 100 person-years (95% CI: 26.06 to 47.37) among infants born to women reporting no previous partner testing (Table 3).
Time to event analysis showed significantly lower risk of HIV acquisition among infants of women with partners who attended clinic or reported that their male partners had been tested for HIV. Antenatal attendance was associated with a 42% lower infant HIV infection risk [hazard ratio (HR) = 0.58; 95% CI: 0.34 to 0.98; P = 0.043] and previous partner testing with a 43% reduction (HR = 0.57; 95% CI: 0.35 to 0.93; P = 0.023). Adjusting for maternal viral load, infection risk remained over 40% lower for infants of women with male attendance [adjusted HR (aHR) = 0.56; 95% CI: 0.33 to 0.98; P = 0.042) and with reported prior partner HIV testing (aHR = 0.52; 95% CI: 0.32 to 0.84; P = 0.008). The significantly lower risk was retained when adjusting for infant feeding in addition to maternal viral load (Table 4).
Overall, no association was found between infant mortality and partner involvement. However, in analyses stratified by HIV infection, partner attendance was associated with increased survival in HIV-uninfected infants. Among HIV-uninfected infants whose mothers' male partners attended clinic, mortality incidence was 4.81 per 100 person-years (95% CI: 2.00 to 11.55). This was lower than incidence among infants of women whose partners did not attend (12.63 per 100 person-years; 95% CI: 8.60 to 18.54) (Table 3). In Cox regression models, HIV-uninfected infants born to women with male attendance had a 58% lower mortality risk (HR = 0.42; 95% CI: 0.19 to 0.95; P = 0.036). Adjusting for infant feeding, mortality was 63% lower with partner attendance (aHR = 0.37; 95% CI: 0.16 to 0.83; P = 0.016) (Table 4).
HIV-infected infants born to women whose partners attended clinic had a mortality incidence of 106.50 per 100 person-years (95% CI: 57.30 to 197.92), which was higher than among HIV-infected infants born to women whose male partners failed to attend (mortality incidence 47.91 per 100 person-years; 95% CI: 30.18 to 76.04) (Table 3). In univariate analysis, HIV-infected infants of women with male attendance had a trend for greater mortality risk compared with those born to women without male attendance (HR = 2.07; 95% CI: 0.95 to 4.49; P = 0.067). This trend was maintained in models adjusting for maternal viral load and infant feeding (aHR = 2.02; 95% CI: 0.89 to 4.59; P = 0.093) (Table 4).
Infants born to women with male attendance had a combined incidence of vertical transmission or death of 22.89 per 100 person-years (95% CI: 15.34 to 34.15). Comparatively, for infants born to mothers without partner attendance, the composite event incidence was 47.33 per 100 person-years (95% CI: 38.94 to 57.52). Among infants born to women reporting previous male HIV testing incidence of HIV acquisition or mortality was 28.89 per 100 person-years (95% CI: 21.42 to 38.95) compared with 48.46 per 100 person-years (95% CI: 37.63 to 62.41) for infants born to women reporting no previous partner testing (Table 3).
The combined adverse outcome risk of vertical transmission or infant mortality was significantly lower with male partner involvement. In Cox regression models, infants born to women with male attendance had a significantly lower risk of the combined outcome (HR = 0.52; 95% CI: 0.33 to 0.81; P = 0.004). In addition, infants of women reporting previous partner testing had a 35% lower risk in comparison with those of women whose partners had reportedly not been tested (HR = 0.65; 95% CI: 0.44 to 0.96; P = 0.029) (Table 4). The increased mean HIV-free survival times with both forms of male partner involvement are illustrated in the Kaplan-Meier plots (Fig. 2). Adjusting for maternal viral load, the combined risk for either vertical transmission or mortality was 45% lower with male antenatal attendance (aHR = 0.55; 95% CI: 0.35 to 0.89; P = 0.012) and 41% lower with previous partner testing (aHR = 0.59; 95% CI: 0.33 to 0.89; P = 0.013). Adjusting for feeding modality and maternal viral load, the combined risk remained significantly lower with antenatal attendance and previous HIV testing (Table 4).
In resource-limited settings, it is important to reduce both infant HIV infection and mortality. Programs are beginning to achieve lower vertical transmission rates; however, infant mortality remains high,5,19 making interventions addressing both of these public health issues necessary to improve infant outcomes. This study demonstrated that infants of HIV-infected women with male partner involvement had a significantly lower risk of HIV infection and greater HIV-free survival compared with infants born to women without male involvement. The associations retained significance after adjusting for maternal viral load and infant feeding modality, 2 of the most important predictors of infant survival and HIV acquisition.20,21 These outcomes are consistent with earlier studies, which have demonstrated enhanced utilization of PMTCT services with partner involvement.8-12 A major difference between this report and previous studies is that rather than assessing a surrogate end point (intervention uptake), we evaluated infant HIV infection and mortality. Thus, our finding of a more than 40% reduction in both the risk of vertical transmission and the composite risk of infant HIV infection or mortality provides key new evidence that male involvement may represent an underutilized public health intervention.
In sub-Saharan Africa, PMTCT guidelines encourage partner HIV testing but do not specifically promote antenatal attendance for partners of HIV-infected pregnant women.22 Consequently, men rarely present to participate in antenatal education or counseling. This is illustrated in previous studies where fewer than 20% of men attended antenatal clinics with their HIV-infected partners.12,23 Research has shown that partners of HIV-infected pregnant women in sub-Saharan Africa are not averse to participating in PMTCT or HIV testing services.24 However, health system barriers exist that prevent male participation and will need to be addressed to achieve the benefits of male involvement for infant health.
In this analysis, women whose partners had been previously tested for HIV had a trend for better adherence to zidovudine and were significantly more likely to formula feed their infants, both of which may have contributed to reduced risk of vertical transmission. Furthermore, the lower infant mortality risk associated with male attendance may stem from increased financial, physical, and/or psychosocial support for the HIV-infected pregnant woman and her infant. It is plausible that men who take part in health care processes (antenatal PMTCT or HIV testing) have more knowledge of and involvement in their families' health and subsequently better support women to prevent infant HIV infection and mortality. Our data provide impetus to further characterize male involvement within maternal and child health programs to harness the benefits that partner involvement provides.
We observed 63% less mortality risk among HIV-uninfected infants born to women whose partners attended clinic compared with those born to women whose partners did not attend. With rising rates of antenatal HIV testing and effective PMTCT interventions, HIV-exposed uninfected infants comprise the majority of children born to HIV-infected mothers. Thus, reducing mortality in this group would have major public health benefits. However, we also observed a concerning trend toward greater mortality risk among HIV-infected infants born to women with partner attendance. It is possible that knowledge of infant HIV infection impacted support for maternal and child care, particularly at the time of this study when prognosis with infant HIV infection was poor, and this merits additional investigation. With the known importance of preventing HIV infection for infant survival in sub-Saharan Africa6 and with improved access to pediatric antiretroviral therapy, the possible trend toward greater risk may be less of an issue.
This study has limitations. For example, we did not assess for any potential negative effects of male involvement. However, domestic violence has been studied by our group in a different antenatal cohort and was not increased with disclosure of HIV serostatus.25 Nonetheless, partner violence in sub-Saharan Africa is highly prevalent, and there has been concern that promotion of serostatus disclosure may result in abuse.26,27 Although current evidence suggests that benefits outweigh risks, monitoring for domestic violence is warranted in future studies. A second limitation is that there may have been response bias when women answered sensitive questions regarding partner serostatus disclosure and testing. Previous partner HIV testing was reported by 52% of women, which is higher than census rates during the period of this study.28 This type of misclassification would bias toward the null and any beneficial relationship between previous testing, and improved infant health would be an underestimation of the association, further strengthening the evidence for promoting male involvement.
Considering the time elapse since this cohort was accrued, it is possible that secular changes in PMTCT and HIV testing may alter the applicability of the observed associations. However, given the dynamic nature of the HIV pandemic and prevention efforts in Africa, this concern applies to all longitudinal research. The significant association in improvement in infant health outcomes with male partner involvement is valid in the setting of this study, and our findings underscore an important proof of concept regarding the beneficial role of partner involvement on infant HIV and mortality. These findings extend and are consistent with previously identified benefits of partner involvement on intermediate markers such as PMTCT intervention uptake.8-12,29,30 Further studies to support the generalizability and better define the impact of male involvement in PMTCT services will be important.
In conclusion, these data suggest that incorporating men into PMTCT programs with associated HIV testing may improve infant health outcomes by reducing both vertical transmission and mortality among uninfected infants. There remains a need to define specific male partner factors associated with enhanced infant health and to address barriers to partner testing and participation in the antenatal setting. With better understanding of these issues, public health programs facilitating male involvement may augment PMTCT services and improve overall infant health, while promoting couple counseling and testing, as well as treatment and prevention efforts in at-risk populations.
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