More than 6000 women in the United States living with HIV become pregnant each year.1 Before 1994, approximately 25% of their infants ultimately became infected with HIV. Results from the AIDS Clinical Trials Group study 076 (ACTG 076) established that administration of zidovudine to the mother during pregnancy and delivery, and to the infant postpartum reduced the rate of HIV transmission from mother to child (MTCT) by two-thirds.2 Highly active antiretroviral treatment (HAART), elective cesarian sections for women with HIV-1 RNA >1000 cp/mL, and avoidance of breastfeeding have subsequently been shown to reduce MTCT rates to <1%-2% for women identified early in pregnancy.3 Unfortunately, the most recent surveillance data from the Centers for Disease Control (CDC) demonstrate up to 4% transmission at sentinel sites.4 Although these data may not be indicative of current trends, they suggest that we are not achieving optimal coverage of HIV-infected pregnant women. Despite the great success in the prevention of mother-to-child transmission (PMTCT) in the United States, more aggressive screening and implementation of effective interventions may be required.
The Women and Infants Transmission Study (WITS) cohort previously found that HIV-1 RNA at delivery is associated with risk for MTCT.3 A prospective analysis from January 1990 to June 2000 showed the odds of transmission increased 2.4-fold (95% CI, 1.7 to 3.5) for every one log10 cp/mL increase in delivery viral load. Other studies support these findings, however, research to date has not correlated the use of HAART with the outcome of viral suppression.5-9 Although data support the use of HAART for PMTCT,3,10,11 prior studies have not fully elucidated the impact of ritonavir boosting on maternal HIV-1 RNA at term.12,13 In addition, few studies have examined the association between specific HAART regimens and HIV-1 RNA during pregnancy while controlling for sociodemographic, racial, and behavioral factors. Instead, most prior research has focused on the impact of these factors on HIV infected women outside of the context of pregnancy.14
Using data from one of the largest and well-characterized prospective cohort studies of infected pregnant women in the United States, we conducted an analysis to determine the clinical, sociodemographic, and biologic risk factors associated with lack of maternal virologic suppression at term in women who were either “HAART-experienced” or “HAART-naive” before pregnancy. We chose to subdivide our sample into these 2 categories to provide proxy measures for “duration of treatment” and because the approaches for managing determinants of lack of suppression might differ between these 2 groups. Our primary aim was to determine the relationship between HIV-1 RNA levels at delivery and HAART regimen in these 2 groups. Our a priori hypothesis was that more potent regimens containing ritonavir would be associated with improved HIV-1 RNA suppression at delivery. Our secondary aim was to examine the impact of known sociodemographic risk factors on HIV-1 RNA while controlling for HAART regimen. We hypothesized that sociodemographic factors that have previously been associated with lower adherence would increase the risk of having a detectable HIV-1 RNA level at delivery, independent of HAART regimen.15,16
The WITS is a multicenter, prospective, natural history cohort study of the perinatal transmission of HIV-1, and the natural history of HIV-1 infection in pregnant women and their infants.17 Starting in December, 1989, HIV-infected pregnant women and their infants were enrolled at centers in New York City, Boston, Worcester, San Juan, and Chicago. Additional sites were added in 1991 in Brooklyn and in 1993 in Houston. The study was approved by each site's institutional review board, and all women provided informed consent for enrollment of themselves and their newborns. The women were enrolled at any time during pregnancy or up to 7 days postpartum.
HIV-infected women were assessed, based upon their time of enrollment, at or before 20 weeks of gestation, at 25 ± 2 weeks, at 32 ± 2 weeks, at delivery, and at 2- and 6-month postpartum visits. At each visit, women completed detailed medical and behavioral questionnaires, underwent physical examination, and gave a sample of blood. CD4+ cell counts were determined in fresh blood by flow cytometry, in accordance with protocols developed by the Division of AIDS of the National Institute of Allergy and Infectious Diseases. Plasma HIV-1 RNA was measured in 5 laboratories from repository samples (stored at −70°C). Obstetric data were obtained from abstraction of the medical record. Antiretroviral therapy was not prescribed as part of the study. Start and stop dates for medications were recorded as the month and year of the first or last use. Only one regimen was categorized per pregnancy, based upon a woman's earliest regimen initiated.
The nested cohort used for this analysis consisted of the subset of women who received HAART during pregnancy and gave birth to infants between June 1998 and December 2005. We chose June 1998 to December 2005 because that corresponded to the funding periods for WITS III and IV, when there were larger numbers of women on 3 drug regimens for PMTCT. We restricted our primary analysis to women who had an HIV-1 RNA level available from delivery. We excluded all women whose “delivery HIV-1 RNA” was recorded as their “enrollment HIV-1 RNA,” to eliminate any women who enrolled during or immediately before delivery. HAART was defined as a regimen of 3 or more antiretroviral drugs. In all cases, women received at least 2 nucleoside reverse-transcriptase inhibitors (NRTIs) combined with either (1) a nonnucleoside reverse-transcriptase inhibitor (NNRTI); (2) an unboosted (without ritonavir) protease inhibitor (PI); (3) a boosted (with ritonavir) PI; or (4) a third NRTI. Two subpopulations of women were analyzed: those who were HAART-experienced before pregnancy and those who were HAART naive before pregnancy. This information was determined based upon self-report from patients. Women classified as HAART-naive before pregnancy could have started on treatment before study enrollment (given they were allowed to enroll at any point during their pregnancy). Given this, a minority of the HAART-naive subset (11%) had undetectable levels of HIV-1 RNA upon study enrollment.
Univariate analyses of associations were performed using the chi-squared test, Fisher exact test, Wilcoxon rank sum test, or F-test from analysis of variance, as appropriate. Covariates considered as potential risk factors for detectable HIV-1 RNA at delivery included sociodemographic characteristics (maternal age at delivery, race, insurance status), factors associated with birth (parity, number of live births), factors associated with disease acuity (CDC clinical classification18 enrollment CD4+ count, and enrollment HIV-1 RNA level), maternal illicit drug use (defined as use of heroine/opiates, methadone, cocaine, or any injection drug use during the current pregnancy), timing of HIV diagnosis and of HAART initiation, and HAART regimen. Logistic regression models estimated the odds of having a detectable HIV-1 RNA level at delivery (>400 cp/mL) as a function of these characteristics. We chose an HIV-1 RNA cutoff of >400 cp/mL because it was the standard assay limit of detection used during the WITS study.
We generated 2 separate multivariable models that examined women who were either HAART experienced or HAART naive before pregnancy. For each multivariable model, we first used stepwise logistic regression with P < 0.05 as the entry and stay criteria for variable selection. We then fit a model for each of the 2 populations which included all variables which were significant in one or both of the models identified using stepwise variable selection. Finally, as we were interested in evaluating potential differences between HAART regimens, we added this variable in the model. Statistical analyses were performed with SAS version 9.1 (SAS Institute Inc, Cary, NC).
Of 935 HIV-1-infected women enrolled between June 1998 and December 2005, 630 (67%) met eligibility criteria for this analysis (Table 1). The majority of women in the analysis cohort were young (59% were <30 years old), black (57%), on public insurance (80%), not illicit drug users (80%), and in either CDC class A or B (93%). Among all 630 women, 109 (17%) received a boosted PI-based regimen (64 women were on lopinavir/ritonavir and the remainder received either indinavir/ritonavir or saquinavir/ritonavir), 402 (65%) received an unboosted PI-based regimen (all containing nelfinavir), 87 (14%) received an NNRTI-based regimen (all containing nevirapine), and 25 (4%) received a triple NRTI-based regimen (the majority with abacavir). Most regimens contained zidovudine (89%) and lamivudine (95%) as part of the NRTI backbone.
Of these, 364 (58%) were HAART experienced before pregnancy and 266 (42%) were HAART naive before pregnancy. A comparison of enrollment characteristics by history of HAART exposure is shown in Table 1. There were significant differences between HAART-experienced and HAART-naive women in age, insurance status, parity, and time because HIV diagnosis (median of 5 years in the HAART-experienced group versus 6 months in the HAART-naïve group). The median CD4+ cell count overall was 435 cells/μL (IQR 294, 640), however, women who were naive to HAART before pregnancy were more likely to have a first CD4+ cell count measurement >200 cells/μL (95% vs. 83%, P < 0.001) and lower CDC class (73% vs. 54% class A, P < 0.001). Among both HAART-experienced and HAART-naive women, 53% had a detectable HIV-1 RNA at first study measurement in pregnancy. There was a significant difference between HAART-experienced and HAART-naive women in the proportion of women using the different types of regimens (P < 0.001, Table 1). This primarily reflected a higher proportion of HAART-experienced compared with HAART-naive women who received a boosted PI regimen. We also examined the distribution of women by year of study enrollment (Fig. 1). The proportion of women with detectable HIV-1 RNA at enrollment varied significantly by calendar year of enrollment, reflecting declining trends over time, both among women who were HAART experienced before pregnancy (P < 0.001) and among women who were naive before pregnancy (P < 0.001).
Among all 630 women, the overall rate of detectable HIV-1 RNA at delivery was 32%. In univariate analyses, significant predictors for detectable HIV-1 RNA at delivery included prepregnancy HAART exposure, younger age at delivery, black race, public insurance, maternal illicit drug use, HIV diagnosis before the current pregnancy, detectable HIV-1 RNA at enrollment, and earlier year of enrollment (Table 2). Parity was not significant and type of HAART regimen was of borderline significance (P = 0.051). In separate analyses of HAART-experienced and HAART-naive women, the directions of associations with these factors remained the same as in the overall analysis, but for HAART-experienced women, only age, race, HIV-1 RNA at enrollment and year of enrollment remained significant, whereas for HAART-naive women, only maternal illicit drug use, timing of HIV diagnosis, and HIV-1 RNA at enrollment were significant. Of 295 women who entered the study with an undetectable HIV-1 RNA, 39 (13%) ultimately had detectable levels of HIV-1 RNA at delivery; 15% among women in the HAART-experienced group, and 11% among women in the HAART-naive group. Conversely, 162 (48%) of the 335 women who were detectable at enrollment remained detectable at delivery; 57% among women in the HAART-experienced group and 36% among women in the HAART-naive group.
Table 3 shows gestational age at delivery and number of women with detectable HIV-1 RNA at enrollment and delivery by treatment regimen for both HAART-experienced and HAART-naive women. In both cohorts, women on PI-based therapies were more likely to have a detectable viral load both at enrollment and at delivery. In univariate analysis, there was no apparent advantage to boosting PI-based regimens with ritonavir.
Results from the multivariable analysis are displayed in Table 4. The variables that were selected for inclusion in the model (see Statistical Methods) are reported in the footnote in Table 4. Among HAART-experienced women, detectable levels of HIV-1 RNA levels at delivery were significantly associated with a higher enrollment HIV-1 RNA [adjusted odds ratio (AOR) 1.52 per log10 cp/mL, 95% confidence interval (CI) 1.32 to 1.75, P < 0.001] and a lower enrollment CD4+ count (AOR 1.20 per 100 cells/μL, CI 1.04 to 1.37, P = 0.009) at first study measurement. Among HAART-naive women, detectable levels of HIV-1 RNA levels at delivery were also associated with a higher HIV-1 RNA at enrollment (AOR 1.35 per log10 cp/mL, CI 1.12 to 1.63, P = 0.002) and a lower CD4+ count at enrollment (AOR 1.24 per 100 cells/μL, CI 1.05 to 1.48, P = 0.014). In addition, maternal age (AOR 0.92 per 10 years older, CI 0.86 to 0.99, P = 0.022) and maternal illicit drug use (AOR 3.15, CI 1.34 to 7.41, P = 0.008) were significantly associated with detectable HIV-1 RNA among HAART-naive women. Type of regimen was not associated with detectable HIV-1 RNA at delivery for either HAART-experienced or HAART-naive women. After adjustment for the other variables included in the model, there was a significant association among HAART-experienced women (P = 0.001), but not among HAART-naive women (P = 0.77), with calendar year of enrollment reflecting a decreasing trend over time in the odds of detectable HIV-1 RNA at delivery.
The use of HAART for PMTCT is one of the most successful public health interventions of the HIV era in the United States.10 Despite rates of transmission remaining below 1% for the last 5 years of WITS, we found that 32% of the women who received HAART between 1998 and 2005 had a detectable HIV-1 RNA at delivery. This discrepancy may indicate successful peripartum prophylaxis efforts of mothers and infants, but it also highlights the fact that one of the cornerstones of PMTCT-maternal virologic suppression-was difficult to achieve in this cohort. MTCT continues to occur in the United States, and this transmission is at least in part due to factors associated with detectable maternal HIV-1 RNA at delivery. Although rates of detectable viral load declined over time, a finding which is also consistent with CDC data,4 the high numbers of women on treatment with detectable HIV-1 RNA highlight the need for increased counseling of known HIV-infected women and aggressive treatment for the most vulnerable groups regardless of pregnancy status, both for PMTCT and overall maternal health.
In the current study, women who were most at risk were those who presented with a higher HIV-1 RNA and lower CD4+ count at enrollment. This was seen among women who were HAART-experienced and among women who were HAART-naive before their pregnancy. For women who were HAART-naive before pregnancy, this may have reflected more advanced disease and/or initiation of therapy later in pregnancy. For women who were HAART-experienced before pregnancy, this may have reflected either more advanced disease possibly refractory to antiretrovirals or suboptimal use of antiretrovirals before pregnancy that was ultimately more resistant to subsequent regimens. Younger age and illicit drug use also significantly increased the odds of having detectable HIV-1 RNA at delivery in women who were HAART-naive before pregnancy.
The type of HAART treatment did not significantly impact HIV-1 RNA levels at delivery, both before and after adjusting for enrollment factors, including HIV-1 RNA and CD4+ count. This finding was consistent for all women. The lack of superiority for boosted PIs was a surprising finding in our evaluation of the impact of antiretroviral regimens on suppression of maternal HIV-1 RNA at term. Although these findings are novel, it is important to interpret them with caution, given the relatively small numbers in each treatment group, and the observational design that may be prone to residual confounding. In addition, boosted PIs may have been used more commonly in patients with more advanced disease, in whom antiretrovirals may have diminished effectiveness. We therefore believe that current treatment practices that recommend the use of ritonavir boosting when using PIs in pregnancy should continue, pending further study of this question, ideally in randomized studies.
The loss of viral load suppression during pregnancy that occurred among 13% of women who initially had undetectable levels of HIV-1 RNA is of concern. It is unknown whether pregnancy itself increases the risk for loss of suppression. Factors specific to pregnancy may include increased medication intolerance (especially gastrointestinal), increased medication side effects (anemia's or other), or altered pharmacokinetics.19-21 Several studies have reported lower area under the plasma concentration time curve in the third trimester with both lopinavir/ritonavir22,23 and nelfinavir,23 but the association between lower drug levels during pregnancy and virologic outcome at delivery has not been studied. Many experts, however, consider it to be reasonable to increase the dose of certain PIs during the third trimester when viral load remains unsuppressed in the setting of good medication adherence.24 Whether such dose increases might have reduced the loss of suppression in this cohort is unknown. We believe our findings warrant further study in trials where information on medication adherence, pharmacokinetics, and resistance can be obtained.
Women who were HAART-experienced before pregnancy differed from those who were HAART naive before pregnancy, both in enrollment characteristics and likelihood of virologic suppression at delivery. In general, women who were HAART experienced were significantly more likely to be older, on public insurance, parous, have a more advanced CDC class, a longer duration of HIV diagnosis, and a lower CD4 count at enrollment. HAART-naive women seemed to have generally less advanced disease at enrollment and were more likely to be virologically suppressed at term. This may in fact reflect disease stage, but could also reflect unmeasured sociodemographic factors, or lower rates of resistant virus among HAART-naive women.
There were some significant trends within the cohort over time. There seemed to be fewer women who were HAART naive at enrollment in later years. This may reflect expanded screening for HIV and earlier treatment outside of pregnancy or more known HIV-infected women choosing to get pregnant. An important observation seen in our multivariable model was that the odds of having a detectable HIV-1 RNA at delivery declined with calendar year in the HAART-experienced group, even after adjustment for HIV-1 RNA and CD4+ cell count at enrollment. This may be reflective of increasing knowledge of the importance of viral load suppression at delivery and greater ability to achieve this in the HAART-experienced group as treatment options expanded, despite minimal changes over time with the HAART-naive group. We did not find, however, that calendar year confounded associations with other factors included in the model.
This was an observational analysis, and prone to biases, including potential selection bias, and unmeasured confounding. We had limited information on medication dosage, patterns of resistance, adherence, and the exact timing of HAART initiation. In addition, we were only able to measure the initial regimen used in pregnancy, and viral suppression at delivery may have been affected by subsequent regimen changes. Because most women had started HAART before study entry, our first measured CD4+ cell count and HIV-1 RNA were not true “baseline” values, even for women who were HAART naive before pregnancy. Despite these limitations, our study is one of the first US-based observational analyses comparing the association between regimens and virologic suppression at delivery in the current era of HAART availability. Virologic suppression at delivery is an important risk factor for MTCT, and one that can be clinically measured. HIV-1 RNA suppression at delivery is a reasonable surrogate measure of success for antiretroviral therapy as an intervention to prevent MTCT.
In conclusion, our analysis suggests that women with lower CD4+ counts and higher HIV-1 RNA during pregnancy are at an increased risk for a detectable HIV-1 RNA at delivery. Persistently detectable HIV-1 RNA is particularly common among HAART-experienced women presenting with detectable HIV-1 RNA in pregnancy (57% of whom remained detectable at delivery). Of additional concern was the loss of virologic suppression by delivery among 13% of women with undetectable HIV-1 RNA at entry. Our findings suggest that efforts to improve suppression at term are warranted, particularly among women with lower CD4+ cell count and higher HIV-1 RNA.
The authors gratefully acknowledge the women and their families who have participated in the WITS and the efforts of the dedicated study personnel at all sites throughout the study who have made this analysis possible. Special thanks to Erin George for help in manuscript preparation and David R. Bangsberg and Alexi Wright for editorial suggestions.
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Principal investigators, study coordinators, program officers, and funding include the following: Clemente Diaz and Edna Pacheco-Acosta (University of Puerto Rico, San Juan, PR; U01-AI-34858); Ruth Tuomala, Ellen Cooper, and Donna Mesthene (Boston/Worcester site, Boston, MA; U01-DA-15,054); Phil La Russa and Alice Higgins (Columbia-Presbyterian Hospital, New York, NY; U01-DA-15053); Sheldon Landesman, Edward Handelsman, and Ava Dennie (State University of New York, Brooklyn, NY; U01-HD-36117); Kenneth Rich and Delmyra Turpin (University of Illinois at Chicago, Chicago, IL; U01-AI-34841); William Shearer, Susan Pacheco, and Norma Cooper (Baylor College of Medicine, Houston, TX; U01-HD-41,983); Joana Rosario (National Institute of Allergy and Infectious Diseases, Bethesda, MD); Kevin Ryan, (National Institute of Child Health and Human Development, Bethesda, MD); Vincent Smeriglio and Katherine Davenny (National Institute on Drug Abuse, Bethesda, MD); and Bruce Thompson (Clinical Trials and Surveys Corporation, Baltimore, MD; N01-AI-85339). The scientific leadership core includes Kenneth Rich (Principal Investigator) and Delmyra Turpin (Study Coordinator; 1-U01-AI-50274-01). Cited Here...