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JAIDS Journal of Acquired Immune Deficiency Syndromes:
doi: 10.1097/QAI.0b013e3181c7d363
Supplement Article

The Prevalence of Transmitted Antiretroviral Drug Resistance in Treatment-Naive HIV-Infected Individuals in China

Liao, Lingjie PhD*; Xing, Hui MD*; Shang, Hong MD, PhD†; Li, Jingyun PhD‡; Zhong, Ping PhD§; Kang, Laiyi MD§; Cheng, Hua PhD*§; Si, Xuefeng MD*; Jiang, Shulin PhD*; Li, Xinping MD*; Shao, Yiming MD, PhD*

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From the *State Key Laboratory for Infection Diseases Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China; †Key Laboratory of Immunology of AIDS, Ministry of Health, the First Affiliated Hospital, China Medical University, Shenyang, Liaoning province, China; ‡AIDS Research Department, Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing, China; and §Department of AIDS and STD, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, China.

The first six authors contributed equally to this paper.

Supported by the Mega-projects of Science Research for the 11th 5-year plan (2008ZX10001-004), the National Basic Research Program (2005CB523103), and the International Cooperation Program (20070FC30230), granted by the Ministry of Science and Technology of China; funds from the China Global Fund AIDS Program Round 3 & 4, the Global Fund to Fight AIDS, Tuberculosis, and Malaria; and the China ICOHRTA with Grant #U2R TW 006918, funded by the US National Institutes of Health, the Fogarty International Center, the National Institute of Drug Abuse and the National Institute of Mental Health.

Presented in part at the 4th IAS Conference on HIV Pathogenesis, Treatment, and Prevention, July 22-25, 2007, Sydney, Australia. Abstract TUPEB051.

Correspondence to: Yiming Shao, MD, PhD, State Key Laboratory for Infection Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China (e-mail:

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Background: Transmission of HIV drug resistance (TDR) gives rise to reduced efficacy of initial antiretroviral treatment and has become a public health concern.

Methods: A nationwide survey on TDR was conducted in antiretroviral therapy-naive HIV-1-infected individuals from September 2004 to October 2005 in China. Drug resistance genotyping was performed on subjects' plasma samples. Drug resistance mutations were determined and scored by Stanford HIV Drug Resistance algorithm.

Results: Sequences were obtained from 676 individuals, of whom 61.2% were former plasma and blood donors, 17.3% were infected sexually, and 17.2% were intravenous drug users. Subtype B' HIV-1 strains were found in 73.5%, CRF01_AE in 13.9%, CRF07_BC in 6.2%, CRF08_BC in 2.7%, subtype C in 1.04%, subtype B in 0.9%, CRF02_AG in 0.4%, and B'/C intersubtype recombinant strains in 1.3% of the subjects. Twenty-six (3.8%) were found to harbor drug resistance strains. The rates of resistance to protease inhibitors, nucleoside reverse transcriptase inhibitors, and nonnucleoside reverse transcriptase inhibitors were 0.4%, 1.6%, and 2.1%, respectively. Though there was no significant difference in TDR rates between 2004 and 2005 (2.9% vs. 4.4%), an increased trend was observed in the rate of high-level drug resistance (0.8% in 2004 vs. 3.0% in 2005, P = 0.0634).

Conclusions: The rate of TDR was relatively low in China, as compared with those in developed countries. Surveys among recently HIV-infected subjects should be performed continually to ensure the success of the scale-up antiretroviral treatment.

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Since the first HIV case was identified in 1985 in China, the HIV/AIDS epidemic kept rising during the subsequent 20 years. By the end of 2005, it was estimated that 650,000 individuals living with HIV/AIDS in China, of whom 75,000 were AIDS patients.1 Intravenous drug use (44.3%), sexual contact (43.6%), and unsafe blood and plasma collection (10.7%) were main transmission routes which contributed to most of the 141,000 HIV cases reported. Furthermore, the distribution of HIV/AIDS cases was not geographically even in China: 5 of 31 provinces and autonomous regions had reported over 10,000 cases, whereas 3 other provinces had less than 100 reported cases.

To improve the life of HIV/AIDS patients and reduce the HIV/AIDS-related morbidity and mortality, the Chinese government put forward a “four free one care” policy in 2003.2 About 5000 patients received a national free antiretroviral therapy (ART) program in its first year of inception. Since then, the number of patients enrolled in the program has increased rapidly. At the end of 2005, more than 22,000 patients were ever involved in the free ART program, of which about 19,200 were still on ART.3 Most of them were HIV-infected former blood and plasma donors (FPDs) living in rural areas of central China. Resources for antiretroviral drugs are limited in China. There is a concern that the prevalence of antiretroviral drug resistance would compromise the effect of current regimens and give rise to treatment failure.

With the scale-up of a free ART program, a nationwide survey was conducted to study the genetic characteristic and antiretroviral drug resistance of HIV type 1 (HIV-1) strains in treatment-naive HIV-infected individuals from 2004 to 2005.

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Study Population

Treatment-naive HIV-1-infected individuals were randomly enrolled in provinces and autonomous regions where free antiretroviral drugs were available at the time the survey was conducted. The duration of sample collection was from September 2004 to October 2005. In 2004, treatment-naive HIV-infected individuals in 14 provinces were included. With the rapid scale-up of the national free ART program, the geographic range of the study was expanded to 28 provinces and autonomous regions in the subsequent year. Subjects who were below 18 years old were excluded from the study. All the subjects gave written informed consent for participation in this study. The survey was reviewed and approved by the institutional review boards and scientific ethical committees at the Chinese Ministry of Health.

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Laboratory Tests

Subjects' whole blood samples were collected and processed in the provincial Centers for Disease Control and Prevention's laboratries. CD4 cell count was measured within 24 hours after sampling. Meanwhile, plasma was separated by centrifugation and instantly stored at −80°C. The plasma samples were then sent in dry ice to the 4 core laboratories of the Chinese HIV Drug Resistance Surveillance Network. Plasma HIV RNA was quantified with real-time nucleic acid sequence based amplification (NASBA) ( bioMerieux, Lyon, France), or COBAS (Roche Applied Science, Penzberg, Germany) according to the manufacturer recommendations. For samples with viral loads equal to or greater than 1000 copies per milliliter, HIV drug resistance (HIVDR) genotyping was carried out by an in-house polymerase chain reaction protocol as previously described.4 HIV-1 pol gene (protease 1-99 amino acids and part of reverse transcriptase 1-250 amino acids) was amplified, purified, and bidirectly sequenced in an ABI3100 sequencer (Applied Biosystems, Foster City, CA). For analysis of HIV-1 drug resistance mutation, each sample sequence was compared with the subtype B consensus sequence in the Stanford HIV Drug Resistance Database ( and was interpreted by HIVdb program. For HIV-1 subtyping, the edited sequences were aligned against reference sequences available at the Los Alamos database ( Phylogenetic trees were constructed by the neighbor-joining method with 1000 bootstrap replicates using Mega 4.0. Bootscaning analyses were performed on samples possibly having unidentified intersubtype recombinant strains by using Simplot 3.5.1.

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Statistical Analysis

The significant differences in categorical variables were analyzed using χ2 test or Fisher exact tests. Statistical significance was defined as a P value <0.05. All the statistical analyses were performed with SAS 9.1 software (SAS Institute, Cary, NC).

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Subjects Included

One thousand one hundred ninety-four ART-naive HIV-1-infected subjects were enrolled (442 in 2004 and 752 in 2005). Two hundred twenty-seven individuals who had a plasma viral load less than 1000 copies per milliliter were excluded from the study, as the possibility of such subjects to be sources of HIV infection would be less than those with a higher viral load. In addition, the genotypic results from samples with a viral load lower than 1000 copies per milliliter were not reliable using routine methods. Of the remaining 967 samples, 676 (69.9%) were successfully sequenced. Among these subjects, 460 (68.1%) were male. Median age was 38 years (range: 18-72). Four hundred fourteen (61.2%) were FPDs, 117 (17.3%) acquired HIV infection through sexual contact, 116 (17.2%) were intravenous drug users, 27 (4.0%) were infected through blood transfusion, and the transmission routes of the remaining 2 subjects were unknown. More than half of the subjects (351 of 676, 51.9%) had a primary school education or less. Four hundred fifty-one (66.7%) were farmers living in rural areas. Median CD4 cells count and viral load at sampling were 280 cells per cubic millimeter (range: 23-723) and 4.59 log copies per milliliter (range: 3.01-6.80), respectively.

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HIV-1 Subtypes, Circulating Recombinant Forms, or Intersubtype Recombinant Strains

Phylogenetic trees base on the whole protease (1-99aa) and part of reverse transcriptase (1-250aa) gene revealed that a majority of the HIV-1 isolates (497, 73.5%) studied belonged to subtype Thai-B (B'), the second most prevalent strain was CRF01_AE (94, 13.9%), followed by CRF07_BC (42, 6.2%), CRF08_BC (18, 2.7%), subtype C (7, 1.04%), subtype B (6, 0.9%), and CRF02_AG (3, 0.4%). Bootscaning analyses were performed on sequences from nine samples, which did not cluster with any present known reference sequences. The results revealed the presence of B'/C intersubtype recombinant strains with different mosaic structures from those of CRF07_BC and CRF08_BC (Fig. 1). The distribution of HIV-1 strains was uneven among different risk groups (P < 0.0001). Subtype B' HIV-1 strains were more frequently found in FPDs (398, 80.1%) than in other HIV-1-infected populations; CRF07_BC strains were more likely to be found among intravenous drug users (IDUs) (27, 64.3%), whereas most CRF01_AE strains were detected in 2 groups: IDUs (46, 39.7%) and sexually infected individuals (41, 35.0%).

Figure 1
Figure 1
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Antiretroviral Drug Resistance-Associated Mutations

Twenty-six of 676 isolates (3.8%) were identified to be resistant to 1 or more antiretroviral drugs (Table 1). Primary drug resistance mutations to protease inhibitors were found in 3 samples (0.4%), which had 1 single mutation each. M46I and N88D, which confer intermediate resistance to nelfinavir, were respectively detected in 1 subject (CNSHD0050) from Shanghai and 1 person (CNHND0066) from Hunan province. A V82A mutation conferring resistance to atazanavir, indinavir, lopinavir (LPV), and nelfinavir was found in 1 subject (CNGXD0011) from Guangxi province.

Table 1
Table 1
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Mutations associated with resistance to reverse transcriptase inhibitors were found in 23 subjects. Twenty-one of them were resistant to either nucleoside reverse transcriptase inhibitors (NRTIs) (n = 9) or nonnucleoside reverse transcriptase inhibitors (NNRTIs) (n = 12); 2 were resistant to both NRTIs and NNRTIs. The most frequent NRTI-related mutation was M184V/I, conferring high resistance to lamivudine and emtricitabine and was found in 4 (4 of 23, 17.4%) samples. At least 1 thymidine analogue mutation, which was associated with intermediate or low-level resistance to zidovudine or other NRTIs, was found in 5 samples. The most frequent NNRTI-related mutation was K103N, which was found in 9 (9 of 23, 39.1%) samples.

Comparison of the HIVDR rate in 3 major risk groups revealed that there was no significant difference among FPDs (13 of 414, 3.1%), sexually infected individuals (5 of 117, 4.3%), and IDUs (4 of 116, 3.5%). No significance difference on transmission of HIV drug resistance (TDR) rate was found between female (7 of 216, 3.2%) and male (19 of 460, 4.1%) subjects, those with primary education or below (3.7%), and those with a higher education (4.0%), or between nonfarmers (5.3%) and farmers (3.1%). The rate of TDR in 2004 and 2005 was 2.9% (7 of 244) and 4.4% (19 of 432), respectively, with no significant difference between the 2 years (P = 0.3207). The rate of high-level resistance to 1 or more antiretroviral drugs was also not significant (P = 0.0634), although there was an increase in 2005 (3.0%, 13 of 432), compared with 2004 (0.8%, 2 of 244).

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Our study was the first nationwide survey on TDR in ART-naive individuals and revealed the existence of transmitted HIV drug resistance in China. Evidence of TDR was found in 3.8% of the subjects studied. This result was similar to results from a previous survey, which found 4.4% of HIV drug resistance of 91 ART-naive HIV/AIDS patients in Liaoning, a province in northeastern China.5 This prevalence was comparable to that of international studies which showed 2.0% TDR during 2003 and 2006 in Thailand,6 4.3% from 1999 to 2005 in South Korea,7 and 4.3% in 2002 in the Democratic Republic of Congo.8 However, the prevalence of TDR in our study was lower than those in North America and Western Europe where various TDR rates were reported to be from 9.1%9 to 24.1%.10 The result was not surprising, as China, like other countries in Asia and Africa, has had a shorter period of access to ART than in developed countries.

In this study, the largest proportion of the HIV-1 strains was subtype B' strains, followed by CRF01_AE, CRF07_BC, CRF08_BC, and other subtypes, circulation recombinant forms or intersubtype recombinant forms. Though the total proportion of 4 predominant HIV-1 strains (subtype B', CRF01_AE, CRF07_BC, and CRF08_BC) and that of CRF01_AE strains alone were similar to what we previously reported on the second nationwide molecular epidemiology survey in 2001 and 2002,11 the proportion of specific HIV-1 strains was different: CRF_BC strains, including CFR07_BC and CRF08_BC, accounted for more than half of the HIV-1 infections, subtype B' only contributed to about one third of the infections in that molecular epidemiology survey. This discrepancy may be due to a sampling bias in favor of regions where access to ART had been already established before the study began. Additionally, the shift of risk groups of HIV infection that had occurred in recent years may produce an influence in the distribution of HIV-1 strains to some extent.12 For this study, subtype B' strains also contributed to a substantial proportion of HIV-1 infections through sexual route and intravenous drug use.

Half of the HIV drug resistance (13 of 26) was found in individuals who were infected by unsafe commercial blood and plasma donation. It was believed that most FPDs acquired HIV infection in the mid-1990s, although a large number of HIV infections were confirmed during the intensive screening of FPDs in 2004. Antiretroviral drugs were not introduced in China during the 1990s. It was therefore impossible that these patients were infected with drug resistance viruses at that time. To avoid including subjects who had a history of ART, the ART status of all subjects with detected drug resistance were reinvestigated by phone or interviewing. About 30 persons with drug resistance were excluded from the study because of having taken antiretroviral drugs. The existence of HIV drug resistance in FPDs in this study include subjects who, infected through intravenous drug use or sexual contact, were reluctant to confess to their mode of transmission due to social stigma.13,14 A portion of FPDs with drug resistance had actually taken antiretroviral drugs at least once, but they were not fully able to understand the questions about their treatment status, or they did not think they had been treated because they only took antiretroviral drugs once or twice. The latter situation would lead to overestimate the prevalence of TDR.

The results of this study was not generalizable to overall HIV infections for the following reasons. First, a large proportion of the subjects were not infected recently. As drug resistance strains become minor quasispecies in the plasma after a period of infection, the regular population-based sequencing method will fail to detect them.15 Second, the selection of the studied population could skew the estimate of the TDR rate in HIV-1 infections. Third, a relative low amplification rate of samples with viral loads equal to or greater than 1000 copies per milliliter may impose an impact on the evaluation of TDR rate. Analysis of the distribution among risk groups revealed that the proportion of the unamplified samples from IDUs and subjects who were infected sexually was higher than those from FPDs (P = 0.0008). However, our data have shown that there was no significant difference on the TDR rate in these 3 populations.

In this survey, we demonstrated the presence of TDR among treatment-naive individuals. More than half of the sample with HIV drug resistance showed resistance to NNRTI drugs. Though the TDR rate was relatively low comparing to those in developed countries, comprehensive measures are needed to prevent the spread of HIV drug resistance. More studies should be performed to investigate the rate of TDR among recent infections and provide implications to first-line therapy.

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We appreciate the contribution of all subjects included in this study. We are greatly grateful to the staff from provincial and county Center for Disease Control and Preventions for participating in sample collection and investigation. We thank Dr. Adrian Liau for his academic editing of the article.

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15. Booth CL, Geretti AM. Prevalence and determinants of transmitted antiretroviral drug resistance in HIV-1 infection. J Antimicrob Chemother. 2007;59:1047-1056.


prevalence; treatment naive; transmitted HIV-1 drug resistance

© 2010 Lippincott Williams & Wilkins, Inc.


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