JAIDS Journal of Acquired Immune Deficiency Syndromes:
Nonalcoholic Fatty Liver Disease Among HIV-Infected Persons
Crum-Cianflone, Nancy MD, MPH*†‡; Dilay, Angelica MPH‡; Collins, Gary MS§; Asher, Dean MD‖; Campin, Richard MD‖; Medina, Sheila BS*†; Goodman, Zach MD¶; Parker, Robin MD‖; Lifson, Alan PhD§; Capozza, Thomas MD#; Bavaro, Mary MD*; Hale, Braden MD, MPH*†; Hames, Charles MD#
From the *HIV Clinic, Naval Medical Center San Diego, San Diego, CA; †Infectious Disease Clinical Research Program (IDCRP), Bethesda, MD; ‡School of Public Health Department, San Diego State University, San Diego, CA; §Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, MN; ‖Radiology Department, Naval Medical Center San Diego, San Diego, CA; ¶Armed Forces Institute of Pathology, Washington, DC; and #Gastroenterology Department, Naval Medical Center San Diego, San Diego, CA.
Received for publication May 23, 2008; accepted November 26, 2008.
Supported by the Infectious Disease Clinical Research Program (IDCRP), Uniformed Services University of the Health Sciences (USUHS), Bethesda, MD, of which the Tri-Service AIDS Clinical Consortium is a component. The IDCRP is a Department of Defense tri-service program executed through USUHS and the Henry M. Jackson Foundation for the Advancement of Military Medicine in collaboration with HHS/NIH/NIAID/DCR through Interagency Agreement HU0001-05-2-0011.
This work is original and has not been published elsewhere. Some data contained in this article were presented as abstract #822 at the 14th Conference on Retroviruses and Opportunistic Infections, February 25-28, 2007, Los Angeles, CA.
The opinions or ascertainments contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the Departments of the Army, Navy, or Air Force or the Department of Defense. The authors have no commercial or other association that might pose a conflict of interest in this work.
Correspondence to: Dr. Nancy Crum-Cianflone, MD, MPH, c/o Clinical Investigation Department (KCA), Naval Medical Center San Diego, 34800 Bob Wilson Drive, Suite 5, San Diego, CA 92134-1005 (e-mail: firstname.lastname@example.org).
Objective: To describe the prevalence and factors associated with nonalcoholic fatty liver disease (NAFLD) among HIV-infected persons not infected with hepatitis C virus (HCV).
Design: A cross-sectional study among HIV-infected patients in a large HIV clinic.
Methods: NAFLD was defined as steatosis among patients without viral hepatitis (B or C) coinfection or excessive alcohol use. The prevalence of NAFLD was identified by ultrasound examination evaluated by 2 radiologists blinded to the clinic information; liver biopsies were performed on a subset of the study population. Factors associated with NAFLD were evaluated by proportional odds logistic regression models.
Results: Sixty-seven of 216 patients (31%) had NAFLD based on ultrasound evaluation. Among those with NAFLD, steatosis was graded as mild in 60%, moderate in 28%, and severe/marked in 12%. Factors associated with the degree of steatosis on ultrasound examination in the multivariate model included increased waist circumference [odds ratio (OR) 2.1 per 10 cm, P < 0.001], elevated triglyceride levels (OR 1.2 per 100 mg/dL, P = 0.03), and lower high-density lipoprotein levels (OR 0.7, per 10 mg/dL, P = 0.03). African Americans were less likely to have NAFLD compared with whites (14% vs. 35%), although this did not reach statistical significance (OR 0.4, P = 0.08). Similar associations were noted for the subset of patients diagnosed by liver biopsy. CD4 cell count, HIV viral load, duration of HIV infection, and antiretroviral medications were not independent risk factors associated with NAFLD after adjustment for dyslipidemia or waist circumference.
Conclusions: NAFLD was common among this cohort of HIV-infected HCV-seronegative patients. NAFLD was associated with a greater waist circumference, low high-density lipoprotein, and high triglyceride levels. Antiretroviral medications were not associated with NAFLD; prospective studies are needed to confirm this finding.
As HIV-infected persons are experiencing longer life expectancies,1 other causes of morbidity and mortality among this group are increasingly being recognized. Recently, liver disease was identified as a leading cause of death among HIV-infected persons.2,3 Patients with HIV infection frequently have elevated liver function tests (LFTs),4 which are often attributed to viral hepatitis (B and C) coinfections or antiretroviral medication effects. The epidemiology of nonalcoholic fatty liver disease (NAFLD) has not been studied among patients with HIV without concurrent hepatitis C virus (HCV) infection but may be an important cause of liver disease in this population.
NAFLD is now recognized as the most common liver disease among the general population since its description by Ludwig et al5 in 1980.6 In the United States, 17%-33% of the population have NAFLD6-8 and its prevalence is likely rising due to increasing prevalence of obesity. NAFLD is defined as the accumulation of lipid droplets (mainly triglycerides) in hepatocytes occurring in the absence of excessive alcohol use or chronic active viral hepatitis.5,9 The disease spectrum ranges from mild steatosis to nonalcoholic steatohepatitis (NASH), advanced stages of fibrosis, cirrhosis, and hepatocellular carcinoma.10,11 Obesity, insulin resistance, diabetes mellitus, and dyslipidemia, which are components of the “metabolic syndrome,”12 are risk factors for NAFLD in the general population and have been referred to as the hepatic component of the metabolic syndrome.13
Data on the prevalence, predictors, and natural history of NAFLD among HIV-infected persons are limited. Before the advent of highly active antiretroviral therapy (HAART), the literature reported steatosis but the etiology was unclear,14-17 and recent studies on steatosis among patients with HIV have been conducted solely among those with HCV coinfection, and HCV itself can result in fat deposition in the liver. In these studies, steatosis was noted in 40%-72% of HIV-HCV-coinfected patients and the proposed risk factors for fatty deposition included nucleoside agents (especially stavudine and didanosine), age, white race, components of the metabolic syndrome, HCV viral load, and HCV genotype 3 infection.18-25
However, patients with HIV without concurrent hepatitis C may be at particular risk for fatty liver disease due to increased prevalence for both risk factors proposed for the “2-hit” pathogenesis of NAFLD: insulin resistance with release of free fatty acids from adipose tissue with subsequent hepatic triglyceride deposition and oxidative stress-cytokine-mediated injury.26 Regarding the first “hit,” patients with HIV often have high rates of lipid and glucose abnormalities, an effect of the HIV infection itself or the antiretroviral medications. In addition to the potential metabolic side effects of antiretrovirals, nucleoside agents may cause direct hepatotoxicity and steatosis due to inhibition of mitochondrial DNA polymerase-γ. Protease inhibitors also may cause steatosis via the overexpression of the sterol regulatory protein, sterol regulatory element binding protein-1.27,28 The rising rates of diabetes and obesity among HIV-infected persons,29,30 similar to the general US population, may also contribute to NAFLD in this population. Regarding the second “hit,” patients with HIV may be at risk due to a chronic inflammatory state (eg, increased tumor necrosis factor-α levels) induced by the virus. In addition, studies have suggested that gut-derived lipopolysaccharide may promote hepatic damage.31
Given the lack of published reports regarding NAFLD among patients with HIV uninfected with HCV, we performed a cross-sectional study among a well-characterized ethnically diverse cohort of HIV-infected patients. Our aims were (1) to determine the prevalence of NAFLD and (2) to identify factors associated with NAFLD among HIV-infected patients without HCV.
Demographics of Study Population
We conducted a cross-sectional study to determine the prevalence and factors associated with NAFLD among patients with HIV uninfected with HCV. Study subjects were HIV-infected patients receiving care at the Naval Medical Center San Diego (NMCSD), San Diego, CA. Patients with HIV (n = 450) attending the clinic are military active duty members, retirees, and dependents. Those on active duty service undergo periodic HIV screening (approximately every 2 years) and routine mandatory drug testing; service members found positive for illicit drugs are discharged from active duty service and are not seen in our clinic. All study participants had confirmed HIV infection by enzyme-linked immunosorbent assay and Western blot testing. Patients were excluded from participating in this study if they were younger than 18 years or were pregnant as determined by a positive urine beta-human chorionic gonadotropin test. All participants provided written informed consent, and the study was approved by the Institutional Review Board at NMCSD.
All patients with HIV meeting the inclusion/exclusion criteria were asked to join the study during their regular clinic visits; enrollment continued until 300 patients signed the informed consent. Study enrollment occurred during the period of January 2006 through June 2007. Of the study group, 257 of 300 patients (86%) had an ultrasound examination performed. Forty-three patients did not have the ultrasound done due to work issues, loss of military benefits, or relocation out of the area. Compared with the characteristics of patients attending this clinic, those who participated in this study were similar in age, race, and military status. Those who joined the study were slightly more likely to be males (93% vs. 88%, P = 0.04) and had a slightly longer duration of HIV (10 vs. 9 years, P = 0.01) than those who did not participate. We also compared those who joined the study who did and did not have an ultrasound examination performed and found no significant differences, except that a lower percentage of females had an ultrasound than those who did not have the test (5% vs. 14% P = 0.04).
Patients had a routine hepatitis panel (hepatitis B surface antigen, hepatitis B core antibody, and hepatitis C antibody tests) at HIV diagnosis; these tests were reviewed as part of this study. In addition, patients with elevated LFTs and a positive hepatitis B core antibody with a negative surface antigen had a hepatitis B DNA drawn. In addition, those with abnormal LFTs and a CD4 cell count of <200 cells per cubic millimeter underwent both a hepatitis B DNA and a hepatitis C RNA viral load testing. Chronic active hepatitis B infection was defined as having a positive surface antigen or having positive core antibody with a detectable hepatitis B DNA viral load. Likewise, a chronic hepatitis C infection was defined as having a positive antibody serology or RNA viral load. Patients found to have chronic hepatitis B (n = 14), chronic hepatitis C (n = 3), or chronic hepatitis B and C (n = 4) were excluded from the analysis. Twenty-one patients who self-reported excessive alcohol use, defined as >140 g ethanol per week for men and >70 g ethanol per week for women,7 were excluded; 1 of the 21 was also excluded for having chronic hepatitis B. This resulted in a total study population of 216 patients with HIV in our study cohort.
Participants completed a questionnaire regarding current symptoms suggestive of liver disease, alcohol and drug use, and medical history. Study coordinators collected data from the patients' medical records on medical diagnoses; past or present receipt of antiretroviral medications (type and duration in months of each medication); and the use of antidiabetic, antihypertensive, lipid-lowering medications and medications that may cause fatty liver disease (eg, corticosteroids, estrogens, amiodarone, valproate, methotrexate, and diltiazem). These data were entered onto study-specific case report forms. Duration of HIV was defined at the date of enrollment minus the midpoint between the date of last HIV seronegative and first seropositive (mean time of seroconversion of 16 months); for those without a documented HIV-seronegative test (n = 54, 25%), the first seropositive date was utilized in this calculation.
At study enrollment, body measurements of weight, height, waist, and hip were performed in a standardized fashion by clinical research coordinators. Body mass index (BMI) was categorized using National Institutes of Health criteria for obesity (≥30 kg/m2), overweight (25.0-29.9 kg/m2), normal weight (18.5-24.9 kg/m2), and underweight (<18.5 kg/m2).32
We also determined the presence of the metabolic syndrome among the participants as defined by the Adult Treatment Panel III (ATP III) guidelines by the presence of ≥3 of the following abnormalities: (1) abdominal obesity (abdominal circumference >102 cm for men and >88 cm for women), (2) elevated triglyceride level (≥150 mg/dL), (3) decreased high-density lipoprotein (HDL) level (<40 mg/dL for men and <50 mg/dL for women), (4) elevated blood pressure, and (5) elevated fasting glucose (≥110 mg/dL).12 Because we did not collect data on the actual blood pressure, we defined elevated blood pressure as the use of an antihypertensive medication.
Laboratory tests including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase, total bilirubin, fasting glucose, total cholesterol, HDL, low-density lipoprotein (LDL), triglycerides, and hepatitis panel were recorded from the closest date to the ultrasound examination. In addition, the most recent CD4 cell count and HIV viral load were recorded at enrollment; an undetectable viral load was defined as <50 copies per milliliter. LFTs were repeated, among those with initially normal results, 6 months (range 3-10 months) later during a routine clinic visit. All blood tests were performed at NMCSD, with the exception of CD4 cell counts that were done at the Veterans Administration Hospital, La Jolla, CA; both laboratories are certified by Clinical Laboratory Improvement Amendments. Lactate levels are not routinely performed in our study population and were not done as part of this study. Data were analyzed using abnormal values of ALT >63 IU/L, AST >41 IU/L, alkaline phosphatase >126 mg/dL, and bilirubin >2.0 mg/dL, which are the upper limits of normal (ULNs) at NMCSD. Bilirubin level was not considered to be elevated if that patient was currently prescribed either indinavir or atazanavir. LFT abnormalities were graded as follows: 1.25-2.5 times ULN (grade 1), 2.6-5 times ULN (grade 2), 5.1-10 times ULN (grade 3), and >10 times ULN (grade 4).
Liver Ultrasound Examination
Each participant underwent a liver ultrasound, which was completed by trained technicians and read by 2 radiologists (D.A., R.C., R.P.) who concurred on the reading and were blinded to the clinical data of the study. US examinations were performed at a mean of 4.2 months (SD 3.5 months) from the time of enrollment/initial blood test; the US examination was followed by a second blood test as described above. Liver size was graded as normal (≤15.5 cm), borderline (15.6-16.0 cm), mild hepatomegaly (16.1-17.5 cm), moderate hepatomegaly (17.6-20.0 cm), or marked hepatomegaly (>20.0 cm). NAFLD was defined by an ultrasound showing steatosis described as diffusion in hepatic echogenicity according to Rumack et al.33 The levels of diffusion for hepatic steatosis were classified accordingly in this study as mild, moderate, severe, and marked.
Liver Histopathologic Examination
Liver biopsies based on medical standards of care were offered to subjects with elevated liver enzymes and/or abnormal ultrasound results; 55 of 165 (33%) agreed to undergo a biopsy. The timing of the biopsy was a mean of 5.2 (SD 3.6) months from the time of the ultrasound examination. Participants who underwent a biopsy were similar to those who did not get a biopsy, except the former group who were more likely to have a higher BMI (28 vs. 26 kg/m2, P < 0.001), to have a greater waist circumference (96 vs. 90 cm, P = 0.005), and to be on lipid-lowering medications (47% vs. 28%, P = 0.04). Each biopsy, stained with hematoxylin and eosin and with Masson trichrome stain, was evaluated for features of fatty liver disease with an expanded version of the NAFLD activity score.34 Specimens that did not contain 5 or more portal tracts were excluded. Biopsies were examined at ×10 and ×40 magnifications with microscopy. Grading of steatosis was categorized as absent (0%), minimal (<5%), mild (5%-33%), moderate (34%-66%), or severe (>66%). NASH was defined as steatosis and parenchymal inflammation with centrilobular pericellular fibrosis and/or hepatocellular ballooning, with or without Mallory-Denk bodies. Biopsies were assessed by a single pathologist (Z.G.) who was blinded to the clinical information of the participants.
The primary outcome of our study was the presence of steatosis (defined as mild, moderate, severe, and marked) on ultrasound examination. We also examined the prevalence of steatosis among the subset of patients with a liver biopsy.
We evaluated the following variables as potential predictors of NAFLD: demographics (age, sex, and race); fasting glucose; fasting lipid levels (total cholesterol, LDL, HDL, and triglycerides); LFTs (ALT, AST, alkaline phosphatase, and total bilirubin); BMI; waist circumference; use of antilipid, antihypertensive, and antidiabetic medications; estimated years of HIV infection; diagnosis of an opportunistic infection; CD4 cell count; HIV viral load; and antiretroviral medication use including duration. Univariate proportional odds logistic regression was used to determine the association of each variable with steatosis level (none, mild, moderate, or marked/severe). Each variable for which the regression coefficient was significant at a P value <0.10 was included in a final multivariate proportional odds model. Correlations between variables were computed using Pearson correlation coefficient. P values of <0.05 and 95% confidence intervals that excluded 1.0 were considered to be statistically significant. Statistical analyses were performed using SAS version 9.1 (SAS Institute Inc, Cary, NC).
Baseline Demographic, Clinical, and Laboratory Characteristics
The mean age of the study population (N = 216) was 40 years (SD 11 years) and 204 (94%) were male; 103 (48%) reported being white, 59 (27%) African American, 30 (14%) Hispanic, and 24 (11%) others (Table 1). Fifty percent of the study population were on active duty military service, whereas 44% were retired and 6% were dependents. Four patients (2%) in the study reported the use of illicit substances. The mean duration of HIV infection was 10.0 years (SD 6.9 years); the mean CD4 cell count was 535 cells per cubic millimeter; and 108 (50%) had an HIV viral load <50 copies per milliliter. At the time of the study, 141 of the patients (65%) were currently prescribed antiretroviral therapy (ART) and 156 (72%) had a history of receiving antiretroviral medications, with 121 (56%), 88 (41%), and 67 (31%) reporting current or prior protease inhibitor, stavudine, or didanosine use, respectively. Only 6 participants (3%) were on medications, which may be associated with fatty liver deposition (2 on estrogens, 2 on prednisone, and 2 on diltiazem).
One hundred fifty-four patients (73%) had dyslipidemia, defined as abnormalities in any of the lipid levels; 33% had an elevated total cholesterol >200 mg/dL; 38% had an LDL >130 mg/dL; 41% had an elevated triglyceride level >150 mg/dL; and 44% had a low HDL <35 mg/dL. Twenty-nine percent of patients were receiving lipid-lowering medications, 23% were receiving antihypertensive medications, and 5% had diabetes mellitus. The mean BMI of the group was 26.0 kg/m2 with 43% being overweight and 15% being obese. Abnormal ALT and AST levels were noted in 27 (13%) and 41 (19%) of the population, respectively. Of the elevated ALT and AST levels, most were grade 1, with only 2 patients having grade 2 elevation, and no patient with grade 3 or grade 4 elevation.
Prevalence of NAFLD
NAFLD was diagnosed in 67 of 216 patients with HIV (31%) based on ultrasound results. Among those with NAFLD, the degree of hepatic steatosis ranged from mild (n = 40, 60%) to moderate (n = 19, 28%) to severe/marked (n = 8, 12%). In addition to steatosis, hepatomegaly was noted in 135 of all study patients (63%) on ultrasound examination: 73 (34%) had mild, 52 (24%) had moderate, and 10 (5%) had marked hepatomegaly. Of patients with NAFLD, 58 (87%) had hepatomegaly. Liver size was strongly associated with NAFLD, with a mean liver size of 16.3 (SD 1.8), 17.4 (SD 1.6), 18.7 (SD 2.2), and 19.3 (SD 4.3) cm among those with no, mild, moderate, and severe steatosis, respectively [odds ratio (OR) 1.8 per 2-cm difference, P = 0.002]. Fifty-five patients with HIV underwent a liver biopsy, of which 20 patients (36%) had biopsy-proven NAFLD. Steatosis on liver biopsy (graded as mild or above) was noted among 11%, 47%, 75%, and 100% of patients with the liver ultrasound, showing no, mild, moderate, or severe steatosis, respectively (Table 2). NASH was present in 20% of the biopsy cases of NAFLD; possible early NASH (steatosis with minimal ballooning and no fibrosis) was documented in an additional 10% of patients with NAFLD.
Symptoms and LFTs Among Patients With HIV With NAFLD
The majority of participants in our study were asymptomatic; the most common complaint was fatigue (24%). Regarding abdominal symptoms, 12% reported diarrhea, 7% loss of appetite, 6% nausea, and 5% right upper quadrant pain. In comparing participants with and without NAFLD, the only symptom that was significantly different was a higher rate of fatigue among those with NAFLD (33% vs. 20%, P = 0.04).
LFTs among patients with NAFLD were usually normal. ALT was elevated, at least once during the study period, among 10 of 67 patients (15%) with NAFLD. Elevated AST occurred in 15 of 67 patients (22%), total bilirubin in 1 of 67 (1.5%), and alkaline phosphatase in no patients with NAFLD. Any abnormality in 1 or more of the LFTs was present in 17 of 67 of patients with NAFLD (25%). Among patients without NAFLD, 38 (26%) had elevated LFTs. In this cohort, the sensitivity and specificity of abnormal LFTs for predicting NAFLD were 25% and 74%, respectively. For each level of steatosis (none, mild, moderate, or severe/marked), the percent with elevated ALT was 11%, 8%, 21%, and 38%, respectively (OR 1.7, P = 0.2).
Factors Associated With NAFLD
The presence of NAFLD was categorized into levels of severity: none, mild, moderate, and severe/marked (Table 1). In the univariate models, variables associated with the degree of NAFLD included higher BMI (OR 2.0, P < 0.001), greater waist circumference (OR 2.2 per 10-cm difference, P < 0.001), lower HDL levels (OR 0.5 per 10 mg/dL difference, P < 0.001), higher triglyceride levels (OR 1.4 per 100 mg/dL difference, P < 0.001), the use of lipid-lowering medication (OR 2.2, P = 0.008), and having the metabolic syndrome (OR 2.2, P = 0.04). In addition, African Americans were less likely to have NAFLD as compared with whites (OR 0.3, P = 0.004). In addition, both overweight (OR 1.8, P = 0.07) and obese patients (OR 3.8, P = 0.001) were more likely to have NAFLD as compared with patients who were underweight or normal weight. The only HIV-related characteristics that showed a potential association with NAFLD were past or current stavudine use (OR 1.8, P = 0.05) and duration of HIV infection (OR 1.2 for every 5 years, P = 0.07). We also evaluated the total duration of stavudine among all patients ever receiving this medication (mean 44 ± 29 months) and found no association (OR 1.1, P = 0.35). Likewise, the total duration of didanosine use among patients (24 ± 27 months) showed no relationship with NAFLD (OR 1.0, P = 0.90). Nadir or current CD4 cell count, HIV viral load, or protease inhibitor use also was not associated with NAFLD. Among those currently receiving HAART, we examined the current CD4 cell count, CD4 change from nadir to current value, and having a suppressed (<50 copies/mL) viral load and found none to be associated with NAFLD (Table 1). Only 6 participants were receiving a non-HIV medication, which may be associated with steatosis; 1 of 6 had steatosis among this group.
Variables included in the multivariate analyses included race, waist circumference, triglyceride level, HDL level, use of lipid-lowering medications, metabolic syndrome, duration of HIV infection, and prior stavudine use (Table 1). Because BMI was highly correlated with waist circumference (BMI: r = 0.78, P < 0.001), and waist size was more highly associated with NAFLD, BMI was excluded from the multivariate model. In the final multivariate model, higher waist circumference (OR 2.1 for each 10-cm difference, P < 0.001), lower HDL levels (OR 0.7 for each 10 mg/dL difference, P = 0.03), and higher triglyceride levels (OR 1.2 for each 100 mg/dL difference, P = 0.03) were associated with NAFLD (Table 1). African Americans compared with whites were less likely to have NAFLD (14% vs. 35%), although this did not reach statistical significance in the multivariate model (OR 0.4, P = 0.08). The potential relationship between stavudine and NAFLD was not seen in the multivariate model when examined by receipt (yes/no) or by duration of use.
The univariate analyses were repeated using the liver biopsy to diagnose NAFLD (Table 3). African Americans compared with whites had a lower prevalence of NAFLD (OR 0.1, P = 0.03). Other predictors included greater waist circumference (OR 3.1 per 10-cm difference, P = 0.001), lower HDL levels (OR 0.3 per 10 mg/dL difference, P = 0.008), and higher triglyceride levels (OR 1.9 per 100 mg/dL difference, P = 0.04). There were no associations between NAFLD and antiretroviral medication use.
NAFLD defined by ultrasound examination is common among patients with HIV occurring at a prevalence rate of 31%. Increased waist circumference, low HDL levels, and elevated triglyceride level were significantly associated with NAFLD, with trends toward a reduced rate of NAFLD among African Americans. HIV-specific factors including antiretroviral medications were not associated with NAFLD in this study of patients with HIV who were uninfected with HCV.
To our knowledge, this is the first study in the HAART era to determine the prevalence of NAFLD among patients with HIV without HCV coinfection. We found that 31% of our study population had NAFLD based on ultrasound imaging. The prevalence of NAFLD in the general population of the United States is similar, with an estimated rate of 17%-33%.7,8 The only other published reports of the prevalence of steatosis among patients with HIV are among patients coinfected with HCV, showing rates of 40%-72%25; however, HCV itself can cause fatty deposition.
Our finding that nearly one third of patients with HIV have NAFLD may have important clinical implications. In the general population, NAFLD may progress to fibrosis, cirrhosis, and liver failure.6,7 Although the natural history of NAFLD among patients with HIV remains unknown, NAFLD is likely an important cause of liver disease in this population. For example, NAFLD as the cause of idiopathic cirrhosis among patients with HIV is increasingly being recognized.35 Furthermore, NAFLD not only causes liver disease but also has been shown in the general population to predict cardiac disease and a decreased survival.6,36,37 Given the high prevalence of NAFLD among patients with HIV seen in this study, the impact of NAFLD as a marker for excess morbidity and mortality among patients with HIV should be prospectively evaluated.
The strongest factor associated with NAFLD in our study was an increased waist circumference, an indicator of central adiposity. Studies of HIV-HCV-coinfected patients also found that high BMI was significantly associated with steatosis but they did not specifically evaluate waist circumference.25 Waist circumference may be a better predictor of obesity-related health risks than BMI38; a study in the general population showed that visceral fat accumulation was predictive of NAFLD regardless of the BMI, including among nonoverweight subjects.39 These findings are likely related to the fact that visceral adipocytes are less mature and more likely to mobilize fat during insulin resistance, which is the first step in the pathogenesis of NAFLD. Furthermore, centrally located fat cells may act as an endocrine organ secreting cytokines and adipokines (eg, adiponectin and leptin) that are important in insulin resistance and fatty liver deposition.40,41 Among patients with HIV, an increased waist circumference may be an effect of both excessive caloric intake and lipohypertrophy.
Our study also suggests that relatively small changes in the BMI may lead to steatosis. The mean BMI measures among those with no, mild, moderate, and severe steatosis in our study were 25, 26, 28, and 32 kg/m2, respectively. In concurrence with our study, a recent investigation among HIV-negative persons showed that the development of steatosis was associated with an increase in BMI of as little as 1 kg/m2, suggesting that small weight changes may produce significant metabolic changes.42 This is particularly of concern in the HIV population in which the rates of elevated BMI seem to be rising. A recent study showed the rates of obesity and overweight were 14% and 31%, respectively,29 and in our population consisting of military beneficiaries, these rates were 15% and 43%, respectively. Given the rising rates of obesity, NAFLD may become increasingly prevalent among HIV-infected persons.
High triglyceride levels and low HDL levels were also associated with NAFLD among our HIV-positive cohort. These findings have biologic plausibility because the pathogenesis of NAFLD involves deposition of triglycerides within the hepatocytes.26,40 Studies among HIV-HCV-coinfected patients and the general population have also found that elevated triglyceride and low HDL levels are independent factors for steatosis.21 Of note, these lipid alterations are common among HIV-positive individuals, likely due to viral influences and antiretroviral medication effects.21,43 Given these data, maintaining triglyceride and HDL levels within a normal range may be an important factor in the prevention of NAFLD among both HIV-positive and HIV-negative persons.
We also noted that African Americans had a trend toward a lower prevalence of NAFLD than whites. Our findings are concurrent with other investigations performed among HIV-HCV-coinfected patients and the general population.19,24,44 These findings are surprising, given that obesity rates are higher among African Americans than whites in the United States.45 In our study cohort, 41% of African Americans were overweight, whereas 15% were obese. With these high rates of obesity, it would be expected that the prevalence of NAFLD in this population would be elevated; however, the contrary has been found in studies to date. Investigations of genetic factors and ethnic differences in lipid homeostasis are advocated to further explore the relationship between African American race and the lower prevalence of NAFLD.
Our study did not find a significant relationship between NAFLD and antiretroviral medications, specifically the nucleoside agents (eg, stavudine and didanosine) and protease inhibitors. A study of NAFLD in HIV-infected children also found no association between ART use and NAFLD.46 Studies to date have been conflicting regarding the impact of HAART on hepatosteatosis among HIV-HCV-coinfected patients with most studies showing no relationship,18-20,23,47,48 whereas 2 studies have showed a relationship between didanosine, stavudine, and protease inhibitor use and steatosis.22,24 Differences in study findings may be due to study population characteristics and the type and duration of antiretroviral use.
Although nearly 75% of our study patients had received antiretroviral medications, the number of patients and the mean duration of stavudine (41%, 44 months) or didanosine (31%, 24 months) use were somewhat limited. This level of usage may not have had a significant effect on the development of liver steatosis as detected by ultrasound evaluation. This is exemplified by a study of patients with HIV-HCV where longer duration of ART use (≥4 years) was found to be an independent predictor of steatosis.21 In addition to the duration of antiretroviral exposure, host genetics (eg, a novel host polymerase mutation) may play an important role in predicting which patients will develop steatosis after receipt of nucleoside agents.49 In summary, whether antiretroviral medications play a direct effect in the pathogenesis of hepatic steatosis and/or an indirect role through their effects on metabolic factors, such as fat accumulation and lipid levels, requires further study. Our study suggests that abdominal obesity and dyslipidemia are the most significant factors in the development of NAFLD among patients with HIV. These metabolic abnormalities may be the result of lifestyle (eg, diet, exercise) factors rather than antiretroviral medications; further studies are needed.
Similarly, we did not find that factors associated with HIV infection, such as duration of HIV infection, HIV viral load, or CD4 cell counts, were associated with NAFLD. Similar results were found in other studies involving HIV-HCV-coinfected patients.25 Speculation exists on the relationship between HIV infection and NAFLD; however, HIV infection is still not a well-established risk factor for NAFLD.
The diagnosis of NAFLD among patients with HIV remains challenging as it is typically a silent disease, with only fatigue being slightly more common among patients with NAFLD. Furthermore, liver enzyme abnormalities are commonly seen in patients with HIV,4 and their sensitivity and specificity for detecting NAFLD were low. The lack of a strong association between ALT level and NAFLD was also noted in other studies,46 with 1 report showing that 79% of patients with NAFLD had normal LFTs.44 We repeated the analysis using lower cut points for ALT (30 mg/dL for men and 19 mg/dL for women)50 and found that the sensitivity for abnormal LFTs increased to 69% but the specificity declined to 46%. Ultrasound examination is a more sensitive test for NAFLD. In addition to steatosis, ultrasound examination frequently detected hepatomegaly, and there was an association between the degree of hepatomegaly and the severity of steatosis in our study. Studies among patients with HIV previously have noted that hepatomegaly may signify underlying steatosis.51 Because NAFLD is usually asymptomatic and has variable LFT results, the diagnosis should be entertained among patients with the associated factors of visceral obesity and hyperlipidemia.
Limitations of our study included that causal relationships could not be assessed due to its cross-sectional design. Because the majority of our cohort were men (94%), our study findings may not be generalizable to women. Self-reporting of alcohol consumption may have been inaccurate, which may have led to incorrectly including or excluding patients within this study. Finally, ultrasonography was used to diagnose NAFLD in our study; the sensitivity and specificity of this test were estimated as 82%-94% and 66%-95%, respectively, compared with liver biopsy results.42,52 Because ultrasound is more sensitive in detecting severe steatosis (>33% fat on the liver),53 we may have underestimated the prevalence of NAFLD in the study population. However, we did perform liver biopsies on a subset of our population and found similar prevalence and predictors of NAFLD.
In summary, NAFLD was common in HIV-infected persons, occurring in one third of the population. This study demonstrated that increased waist circumference, low HDL levels, and high triglyceride levels were predictors of NAFLD. These findings are relevant to both clinicians and patients as early recognition and management of these risk factors among patients with HIV may prevent further progression of NAFLD and morbidity from liver disease among the HIV population.
1. Palella FJ Jr, Baker RK, Moorman AC, et al; HIV Outpatient Study Investigators. Mortality in the highly active antiretroviral therapy era: changing causes of death and disease in the HIV outpatient study. J Acquir Immune Defic Syndr
2. Bica I, McGovern B, Dhar R, et al. Increasing mortality due to end-stage liver disease in patients with human immunodeficiency virus infection. Clin Infect Dis
3. Weber R, Sabin CA, Friis-Moller N, et al. Liver-related deaths in persons infected with the human immunodeficieny virus: the D:A:D study. Arch Intern Med
4. Pol S, Lebray P, Vallet-Pichard A. HIV infection and hepatic enzyme abnormalities: intricacies of the pathogenic mechanisms. Clin Infect Dis
5. Ludwig J, Viggiano TR, McGill DB, et al. Nonalcoholic steatohepatitis: Mayo Clinic experiences with a hitherto unnamed disease. Mayo Clin Proc
6. Adams LA, Lymp JF, St Sauver J, et al. The natural history of nonalcoholic fatty liver disease: a population-based cohort study. Gastroenterology
7. Farrell GC, Larter CZ. Nonalcoholic fatty liver disease: from steatosis to cirrhosis. Hepatology
8. Neuschwander-Tetri BA, Caldwell SH. Nonalcoholic steatohepatitis: summary of an AASLD Single Topic Conference. Hepatology
9. Sanyal AJ. AGA technical review on nonalcoholic fatty liver disease. Gastroenterology
10. McCullough AJ. Pathophysiology of nonalcoholic steatohepatitis. J Clin Gastroenterol
11. Matteoni CA, Younossi ZM, Gramlich T, et al. Nonalcoholic fatty liver disease: a spectrum of clinical and pathological severity. Gastroenterology
12. Executive summary of the third report of the National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high cholesterol in adults (Adult Treatment Panel III). JAMA
13. Pagano G, Pacini G, Musso G, et al. Nonalcoholic steatohepatitis, insulin resistance, and metabolic syndrome: further evidence for an etiologic association. Hepatology
14. Poles MA, Dieterich DT, Schwarz ED, et al. Liver biopsy findings in 501 patients infected with human immunodeficiency virus (HIV). J Acquir Immune Defic Syndr Hum Retrovirol
15. Grumbach K, Coleman BG, Gal AA, et al. Hepatic and biliary tract abnormalities in patients with AIDS. Sonographic-pathologic correlation. J Ultrasound Med
16. Beale TJ, Wetton CW, Crofton ME. A sonographic-pathological correlation of liver biopsies in patients with the acquired immunodeficiency syndrome (AIDS). Clin Radiol
17. Trojan A, Kreuzer KA, Flury R, et al. Liver changes in AIDS. Retrospective analysis of 227 autopsies of HIV-positive patients. Pathologe
18. Neau D, Winnock M, Castéra L, et al; Groupe d'Epidémiologie Clinique du SIDA en Aquitaine. Prevalence of and factors associated with hepatic steatosis in patients coinfected with hepatitis C virus and HIV: Agence Nationale pour la Recherche contre le SIDA et les hépatites virales CO3 Aquitaine Cohort. J Acquir Immune Defic Syndr
19. Marks KM, Petrovic LM, Talal AH, et al. Histological findings and clinical characteristics associated with hepatic steatosis in patients coinfected with HIV and hepatitis C. J Infect Dis
20. Bani-Sadr F, Carrat F, Bedossa P, et al; ANRS HC02-Ribavic Study Team. Hepatic steatosis in HIV-HCV coinfected patients: analysis of risk factors. AIDS
21. Gaslightwala I, Bini EJ. Impact of human immunodeficiency virus infection on the prevalence and severity of steatosis in patients with chronic hepatitis C virus infection. J Hepatol
22. McGovern BH, Ditelberg JS, Taylor LE, et al. Hepatic steatosis is associated with fibrosis, nucleoside analogue use, and hepatitis C virus genotype 3 infection in HIV-seropositive patients. Clin Infect Dis
23. Monto A, Dove LM, Bostrom A, et al. Hepatic steatosis in HIV/hepatitis C coinfection: prevalence and significance compared with hepatitis C monoinfection. Hepatology
24. Sulkowski MS, Mehta SH, Torbenson M, et al. Hepatic steatosis and antiretroviral drug use among adults coinfected with HIV and hepatitis C virus. AIDS
25. Zeremiski M, Talal AH. Dideoxynucleoside analogues should be used cautiously in patients with hepatic steatosis. Clin Infect Dis
26. Day CP, James OF. Steatohepatitis: a tale of two “hits”? Gastroenterology
27. Riddle TM, Kuhel DG, Woollett LA, et al. HIV protease inhibitor induces fatty acid and sterol biosynthesis in liver and adipose tissues due to the accumulation of activated sterol regulatory element binding proteins in the nucleus. J Biol Chem
28. Lemoine M, Barbu V, Girard PM, et al. Altered hepatic expression of SREBP-1 and PPARgamma is associated with liver injury in insulin-resistant lipodystrophic HIV-infected patients. AIDS
29. Amorosa V, Synnestvedt M, Gross R, et al. A tale of 2 epidemics: the intersection between obesity and HIV infection in Philadelphia. J Acquir Immune Defic Syndr
30. Brown TT, Li X, Cole SR, et al. Antiretroviral therapy and the prevalence and incidence of diabetes mellitus in the multicenter AIDS cohort study. Arch Intern Med
31. Wigg AJ, Roberts-Thomson IC, Dymock RB, et al. The role of small intestinal bacterial overgrowth, intestinal permeability, endotoxaemia, and tumour necrosis factor alpha in the pathogenesis of non-alcoholic steatohepatitis. Gut
32. Clinical guidelines on the identification, evaluation, and treatment of overweight and obesity in adults-The evidence report. National Institutes of Health. Obes Res
33. Rumack CM, Wilson S, Charboneau WJ, et al. Diagnostic Ultrasound
. Vol 1. 3rd ed. St Louis, MO: Elsevier Mosby; 2005.
34. Ishak K, Baptista A, Bianchi L, et al. Histological grading and staging of chronic hepatitis. J Hepatol
35. Loulergue P, Callard P, Bonnard P, et al. Hepatic steatosis as an emerging cause of cirrhosis in HIV-infected patients. J Acquir Immune Defic Syndr
36. Arslan U, Türkoğlu S, Balcioğlu S, et al. Association between nonalcoholic fatty liver disease and coronary artery disease. Coron Artery Dis
37. Ekstedt M, Franzén LE, Mathiesen UL, et al. Long-term follow-up of patients with NAFLD and elevated liver enzymes. Hepatology
38. Janssen I, Katzmarzyk PT, Ross R. Waist circumference and not body mass index explains obesity-related health risk. Am J Clin Nutr
39. Eguchi Y, Eguchi T, Mizuta T, et al. Visceral fat accumulation and insulin resistance are important factors in nonalcoholic fatty liver disease. J Gastroenterol
40. Machado M, Cortez-Pinto H. Non-alcoholic steatohepatitis and metabolic syndrome. Curr Opin Clin Nutr Metab Care
41. Diehl AM, Li ZP, Lin HZ, et al. Cytokines and the pathogenesis of nonalchoholic steatohepatitis. Gut
42. Hamaguchi M, Kojima T, Takeda N, et al. The metabolic syndrome as a predictor of nonalcoholic fatty liver disease. Ann Intern Med
43. Jacobson DL, Tang AM, Spiegelman D, et al. Incidence of metabolic syndrome in a cohort of HIV-infected adults and prevalence relative to the US population (National Health and Nutrition Examination Survey). J Acquir Immune Defic Syndr
44. Browning JD, Szczepaniak LS, Dobbins R, et al. Prevalence of hepatic steatosis in an urban population in the United States: impact of ethnicity. Hepatology
45. Ogden CL, Carroll MD, Curtin LR, et al. Prevalence of overweight and obesity in the United States, 1999-2004. JAMA
46. Albisetti M, Braegger CP, Stallmach T, et al. Hepatic steatosis: a frequent non-specific finding in HIV-infected children. Eur J Pediatr
47. Bäuerle J, Miquel R, Laguno M, et al. Hepatic steatosis with stavudine in HIV/hepatitis C co-infection [letter]. AIDS
48. Castéra L, Loko MA, Le Bail B, et al; Groupe D'epidémiologie Clinique du Sida en Aquitaine (GECSA). Hepatic steatosis in HIV-HCV coinfected patients in France: comparison with HCV monoinfected patients matched for body mass index and HCV genotype. Aliment Pharmacol Ther
49. Yamanaka H, Gatanaga H, Kosalaraksa P, et al. Novel mutation of human DNA polymerase gamma associated with mitochondrial toxicity induced by anti-HIV treatment. J Infect Dis
50. Prati D, Taioli E, Zanella A, et al. Updated definitions of healthy ranges for serum alanine aminotransferase levels. Ann Intern Med
51. Frieman JP, Helfert KE, Hamrell MR, et al. Hepatomegaly with severe steatosis in HIV-seropositive patients. AIDS
52. Joseph AE, Savery muttu SH, al-Sam S, et al. Comparison of liver histology with ultrasonography in assessing diffuse parenchymal liver disease. Clin Radiol
53. Saadeh S, Younossi ZM, Remer EM, et al. The utility of radiological imaging in nonalcoholic fatty liver disease. Gastroenterology
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