JAIDS Journal of Acquired Immune Deficiency Syndromes:
Epidemiology and Social Science
High Prevalence of Unique Recombinant Forms of HIV-1 in Ghana: Molecular Epidemiology From an Antiretroviral Resistance Study
Delgado, Elena PhD*; Ampofo, William Kwabena PhD, BSc†; Sierra, María PhD*; Torpey, Kwasi MBChB, MPH‡; Pérez-Álvarez, Lucía MD, PhD*; Bonney, Evelyn Yayra MPhil, BSc†; Mukadi, Ya Diul MD, MPH§; Lartey, Margaret MBChB¶; Nyarko, Charles MD‖; Amenyah, Richard Noamesi MBChB, MPH‡; Thomson, Michael M MD, PhD*; Nájera, Rafael MD, PhD*
From the *Viral Pathogenesis Department, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; †Virology Department, Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Accra, Ghana; ‡Public Health Programs, Family Health International, Accra, Ghana; §Family Health International, Arlington, VA; ¶Department of Medicine, Korle Bu Teaching Hospital, College of Health Sciences, University of Ghana, Accra, Ghana; and ‖St. Martin's Des Porres Hospital, Agomanya, Ghana.
Received for publication March 3, 2008; accepted May 15, 2008.
E.D. and W.K.A. contributed equally to this article.
Supported by the Family Health International's START Project in Ghana, which was funded by FHI corporate funds, as part of the Implementing AIDS Prevention and Care (IMPACT) Project, funded by the U S Agency for International Development through cooperative Agreement HRN-A-00-00017-00. Also supported by Plan Nacional sobre el SIDA, Ministerio de Sanidad y Consumo, Madrid, Spain (Grant MVI 1434/05-2-A).
Part of this work has been presented at the 3rd IAS Conference on HIV Pathogenesis and Treatment, July 24-27, 2005, Rio de Janeiro, Brazil. Abstract no. MoPe14.1B06.
Correspondence to: Elena Delgado, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo, Km. 2, 28220 Majadahonda, Madrid, Spain (e-mail: email@example.com).
Background: In Ghana, programs to expand antiretroviral access are being implemented. In this context, the dynamic genetic evolution of HIV-1 requires continuous surveillance, particularly when diverse genetic forms co-circulate.
Methods: Phylogenetic and antiretroviral resistance analyses of HIV-1 partial pol sequences from plasma RNA samples from 207 Ghanaian individuals were performed.
Results: 66% of infections were CRF02_AG, whereas 25% were unique recombinant forms (URFs). All 52 URFs were characterized by bootscanning. CRF02_AG was parental strain in 87% of URFs, forming recombinants with genetic forms circulating in minor proportions: CRF06_cpx, sub-subtype A3, CRF09_cpx and subtypes G and D. Two triple recombinants (CRF02_AG/A3/CRF06_cpx and CRF02_AG/A3/CRF09_cpx) were identified. Antiretroviral resistance analyses revealed that six individuals, five of which were antiretroviral drug-experienced, harbored mutations conferring high level of resistance to reverse transcriptase inhibitors. No major resistance mutations were identified in the protease, although insertions of one and three amino acids were detected.
Conclusions: The high frequency of URFs detected probably reflects a significant incidence of coinfections or superinfections with diverse viral strains, which increases the genetic complexity of the HIV-1 epidemic in West Africa. Monitoring of HIV-1 drug resistance might provide data on the implications of intersubtype recombination in response to antiretrovirals.
The origin of HIV-1 pandemic has been located in Central Africa.1 Since then, viral strains of group M have evolved into a growing list of subtypes, sub-subtypes, and intersubtype recombinants.2 The genetic diversity of HIV-1 strains circulating in the neighboring West African countries is being revealed with important implications on viral transmission, antiretroviral response, and vaccine design.3-9 The cocirculation of different HIV-1 genetic forms, together with the frequently unknown HIV status of infected individuals, favors reinfections with genetically diverse viral strains. In this context, the extraordinarily high rate of viral genetic recombination facilitates the generation of intersubtype recombinants, which are becoming the main contribution to a rapid increase of HIV-1 genetic variability.10 Those intersubtype recombinants that are identified in 3 or more epidemiologically unlinked individuals are designated as circulating recombinant forms (CRFs), and some of these are predominant in certain geographic areas.11 Unique recombinant forms (URFs) are acquiring epidemiological relevance in the spread of HIV-1 in West Africa, as reported in Burkina Faso, Chad, Niger, Cameroon, Cote D'Ivoire, and Senegal4-9 This dynamic evolution of HIV-1 in West Africa requires continuous surveillance as new genetic forms are being described.
Ghana is a West African country with 320,000 people estimated to be living with HIV and with a prevalence rate of 2.3% among adults.12 Similar to its neighboring countries, where CRF02_AG is the dominant genetic form (between 39% and 83%),13 63% of HIV-1 infections in Kumasi, the second largest city of Ghana, were reported to be caused by CRF02_AG.14 This strain was found to recombine with other genetic forms in 20% of infections, whereas 5% were G/CRF06_cpx recombinants. After publication of this report, new genetic forms of HIV-1 have been described. Furthermore, antiretroviral therapy (ART) is expanding systematically with increased access at various health facilities throughout the country.15,16 However, there is still inadequate information on antiretroviral susceptibility of the viral strains circulating in the country, as only a few studies provide descriptions on susceptibility of Ghanaian HIV-1 strains to ART.17-20
In this study, we analyzed the genetic characteristics of the protease (PR) and reverse transcriptase (RT) coding sequences of HIV-1 from infected patients, at the time when antiretroviral treatments were initiated in Ghana. As a result, we have obtained both a genotypic resistance profile and a detailed phylogenetic outline for an updated contribution to the molecular epidemiology of HIV-1 infections in Ghana.
MATERIALS AND METHODS
Patients and Samples
Blood samples were obtained from 207 Ghanaian HIV-1-seropositive persons accessing the national antiretroviral treatment program between 2002 and 2004 at 3 health facilities: St Martin's Hospital (Agormanya), Atua Government Hospital (both in the Eastern Region), and Korle Bu Teaching Hospital (in Accra, Greater Accra Region). These samples were collected as part of the routine clinical management of patients with HIV to inform initiation of ART. The HIV status of these patients was routinely ascertained by screening of blood samples for HIV antibody with rapid tests: Abbott Determine HIV-1/2 (Abbott Diagnostics, Abbott Park, IL) with confirmation of HIV type by INNO-LIA HIV I/II (Innogenetics, Gent, Belgium). The immune status of the patients was assessed by T CD4+ cell counts with the FACSCount system (Becton Dickinson, Franklin Lakes, NJ), and plasma was stored at −70°C for further analyses.
RNA Isolation, Amplification, and Sequencing
Viral RNA was extracted from 1 mL of plasma with Nuclisens (BioMerieux, Marcy l'Etoile, France), in accordance with the manufacturer's protocol. An HIV-1 pol fragment (HXB2 positions 2080-3662) was amplified by reverse transcription, followed by nested polymerase chain reaction from plasma RNA using an in-house method.21 Before sequencing, the polymerase chain reaction products were purified by digestion with exonuclease I and shrimp alkaline phosphatase (USB, Cleveland, OH). Direct sequencing in PR and RT coding regions was done with ABI Prism BigDye Terminator Cycle Sequencing kit and an ABI Prism 3700 DNA Analyzer (Applied Biosystems, Foster City, CA).
The sequences obtained were assembled with the SeqMan program (DNAStar, Madison, WI), and manually aligned using BioEdit (http://www.mbio.ncsu.edu/BioEdit/bioedit.html). Neighbor-joining trees, based on Kimura's 2-parameter distances, were generated using MEGA 3.1.22 The reliability of tree topologies was assessed by bootstrapping with 1000 replicates, with a bootstrap support of 70%, or greater, being required to define a phylogenetic cluster. Recombination analyses were performed with bootscanning using Simplot v. 184.108.40.206 In this analysis, windows of 250 nucleotides (nt) were used moving in 20 nt increments. Phylogenetic trees were constructed with the neighbor-joining method, using Kimura's 2-parameter distances, with the transition/transversion ratios estimated from the dataset using FindModel (http://hiv-web.lanl.gov/content/hiv-db/findmodel/findmodel.html).
Antiretroviral Resistance Analysis
The PR-RT sequences obtained were analyzed for antiretroviral resistance by the HIVdb program from Stanford University (http://hivdb6.stanford.edu/).24 Mutation frequencies were compared at the HIV Mutation list from Stanford University (http://hivdb6.stanford.edu/asi/deployed/hiv_central.pl?program=hivseq&action=showMutationForm).
Nucleotide Sequence Accession Numbers
Sequence data have been deposited in GenBank under accession numbers EU177879 to EU178085.
Phylogenetic Diversity of HIV-1 in Ghana
Phylogenetic analyses of the pol region of HIV-1 revealed the following subtype distribution of the 207 Ghanaian samples: 136 (65.7%) patients were infected with CRF02_AG, 8 (3.9%) with CRF06_cpx, 5 (2.4%) with sub-subtype A3, 3 (1.4%) with subtype G, and 2 (1.0%) with CRF09_cpx and 1 patient was infected was with subtype B. Fifty-two (25.1%) samples were identified as unique recombinants: 17 (8.2%) were CRF02/CRF06, 16 (7.7%) CRF02/A3, 5 (2.4%) CRF02/CRF09, 3 (1.4%) CRF02/G, 2 (1.0%) CRF02/D, and one each of CRF06/CRF09, CRF06/A3, CRF09/A3, CRF09/G, CRF09/D, A3/D, and A3/G. Finally, 2 patients had HIV-1 strains, which were triple recombinants CRF02/A3/CRF06 and CRF02/A3/CRF09, respectively (Fig. 1). Neighbor-joining trees including only pure HIV-1 subtypes and CRFs are shown in Figure 2. Three patients, GH35, GH55 and GH220, had CRF02_AG/A3 recombinants with coincident breakpoint locations; however, unavailability of epidemiological data prevents us from proposing a putative new CRF. All other URFs had unique mosaic patterns in pol, as shown in Figure 3. A BLAST search was performed to carry out comparative phylogenetic analysis with sequences from Los Alamos HIV Sequence Database.2 We did not find any similar recombination pattern in samples from the database.
CRF02_AG is the dominant genetic form of HIV-1 in Ghana, and it is parental of 87% of the URFs characterized in this study. Nevertheless, most of the other genetic forms, particularly A3 and CRF09_cpx, are found more frequently as part of URFs than as pure genetic forms (Fig. 1).
Reverse Transcriptase Drug Resistance Mutations
Reverse transcriptase drug resistance-associated mutations were detected in 20 patients. Six of them harbored mutations associated with high resistance level to RT inhibitors. Five of these patients had experienced ART before sampling for this study, and these high-resistance mutations correlated with their treatment regimens: the mutation M184V was present in 4 lamivudine-treated patients, T215Y was found in a zidovudine-treated patient, and 3 nevirapine-treated subjects had developed resistance mutations to nonnucleoside RT inhibitors (NNRTIs) (K103N and Y181C). The only HIV-1 subtype B sample identified in this study carried the mutation Y188L, which confers a high level of resistance to NNRTIs; however, in this patient, ART had been initiated after sampling for this study. Other mutations associated with intermediate, potential, or low-level resistance to nucleoside RT inhibitors (M41L, D67E, T69N/A, L210W, and K219T) or to NNRTIs (V108I, V179E/I, P225A, and K238T) were mostly detected among ART-naive subjects. Additionally, polymorphisms at resistance-related positions (T69S, K103R, and L210M) were found in 3 subjects. A complete list of the mutations associated with resistance to antiretroviral drugs that were found in the patients is given in Table 1.
Protease Drug Resistance Mutations
Although no major ART-resistance mutations were detected in the PR region, polymorphisms at resistance-associated positions were frequent, most of them subtype related. Mutations K20I and M36I were detected in 86% and 96% of the patients, respectively. Mutation V82I, which is common among subtype G strains, was found in 17 (8%) patients, including all subtype G samples. L10V, L10I, and V11I were detected in 25 (12%), 16 (8%), and 8 (4%) patients, respectively. Mutations A71T and L89V were detected in 1 patient each. More than 90% of the sequences had 3 polymorphisms in PR (I13V, M36I, and H69K) that are associated with resistance to tipranavir.25
Proteases from 2 patients (GH181 and GH162) had 1 insertion each of 1 (Ser) and 3 (Ala-Asn-Leu) amino acids after positions 37 and 35, respectively. Both samples were classified as CRF02_AG.
In this study, phylogenetic and antiretroviral resistance analyses were combined to determine the genetic profile of HIV-1 strains that circulated in Ghana at the start of the national ART program for HIV.
The analysis of the PR-RT regions of HIV-1 samples from 207 infected patients gave the following genetic classification: 66% were CRF02_AG, 25% URFs, and 9% minority “pure” genetic forms, including CRF06_cpx, sub-subtype A3, CRF09_cpx, and subtypes G and B. Three samples from different individuals shared the same mosaic pattern, comprising sub-subtype A3 and CRF02_AG, suggesting the existence of a new CRF. Each unique mosaic pattern was characterized by bootscanning. The diversity of URFs indicates the possible key role of superinfections or coinfections in this population. Also, it reflects that the genetic proximity of locally circulating genetic forms could favor recombination in the pol gene.
This distribution of HIV-1 genetic forms found in Ghana is concordant with reports derived from neighboring West African countries, where the proportion of URFs detected was between 9% and 27%.5-7,26 However, in those studies, multiple regions of each genome were sequenced to identify recombination. In this study, CRF02_AG was found to be parental of 87% of the URFs characterized. Mosaics involving recombination of CRF02_AG with CRF06_cpx, CRF09_cpx, subtype D, subtype G, or sub-subtype A3 have also been identified in other West African countries.4,8,9,26,27 However, this is the first report in which A3/G, A3/D, CRF09_cpx/CRF06_cpx, CRF09_cpx/G, and CRF09_cpx/D mosaics are described, including 2 triple recombinants. These data reveal the epidemiological relevance of second-generation recombinants in which different CRFs are involved.7
Multiple factors have to be considered to better understand this high prevalence of URFs. First, in West Africa, there is a wide cocirculation of different genetic forms, which may be due to the geographic proximity to the origin of initial expansion of HIV-1. Second, a low genetic distance between the cocirculating genetic forms could have facilitated recombination between homologous sequences.28,29 In fact, the CRF02_AG and CRF06_cpx are related to subtypes A and G, and CRF09_cpx could share ancestors with CRF02_AG.30 Third, these recombinants could have higher fitness than their parental genetic forms, as reported for CRF02_AG.31,32 Fourth, infections with CRF02_AG strains are characterized by high viral loads, which facilitate virus transmission.33 Fifth, these results could reflect a high frequency of multiple infections, in part due to transactional sex, that has been reported as the driving force in the dynamics of HIV-1 infection in Accra, Ghana.34 The abundance of unique recombinants could also reflect a low cross-clade immunological protection between cocirculating genetic forms. In particular, the high degree of recombination of CRF02_AG reported in this study could be favored by a founder effect, where the subsequent introduction of the minority genetic forms frequently resulted in recombinants with CRF02_AG. In fact, it seems that some low-prevalence subtypes (F, G, H, J, and K) are spreading as part of recombinant strains more than as pure subtypes, possibly due to better fitness of the former.
It is possible that these observations could be an underestimation of actual recombination in HIV-1, as only a fragment of about 1100 nt in pol was analyzed by bootscanning. In fact, analyzing near full-length genomes of 7 samples, we found that sample GH142, which is nonrecombinant A3 in pol, recombines with CRF02_AG in env.35 Despite the underestimation, our results are comparable with those reported previously14: 63% (vs 66% in our report) CRF02_AG, 21% (vs 22%) recombinants containing CRF02_AG, and 16% (vs. 8%) other recombinants. In that report, 3 genes (gag, pol, and env) were partially sequenced, whereas in the present study, a longer fragment in pol has been sequenced, and the analysis has been improved by using bootscanning. Furthermore, CRF09_cpx and sub-subtype A3, which had not been characterized at the time of the previous publication, have now been included in the phylogenetic analyses. In fact, a recent report reveals that sub-subtype A3 has experienced a rapid rise in seroincident cases of HIV-1 in Senegal.3
Although full-length genome sequencing is the gold standard to characterize recombination, pol sequencing is useful for both screening for genetic diversity and analyzing antiretroviral susceptibility. In fact, analyzing the 31 CRFs with available mosaic patterns,2 27 (87%) of them show recombination breakpoints in pol. Therefore, analysis of recombination in pol is an accurate and cost-effective approach to conduct subtype classification, which will, in addition, provide information on antiretroviral susceptibility of HIV-1. The high recombination frequency in pol, also observed previously,28,29 could play a key role in transmission of resistance mutations.
Most studies on HIV-1 antiretroviral (ARV) resistance in Africa have been performed in ARV- naive populations. In this study, most of the patients started ART after sampling, but 5 patients with high resistance level to RT inhibitors had ART previously. Nevirapine usage for prevention of mother to child transmission of HIV could be related to some of the NNRTI resistance mutations observed. We report 2 possible cases of transmission of drug resistance in ARV- naive patients (GH101 and GH124) who harbored viral strains with mutations conferring high or intermediate resistance level to NNRTIs. In patient GH124, the clinical records indicate no ART before sampling. A few days after blood was sampled, this patient started a triple ART (zidovudine, lamivudine, and efavirenz), and, subsequently, he experienced treatment failure, probably due to the presence of mutation Y188L. Afterward, the NNRTI was replaced by a ritonavir-boosted protease inhibitor (PI).
Different insertions in PR at a region between codons 35 and 38 have been described previously. Most of them are not related to a change in susceptibility to PIs, although they could contribute to a better fitness and present advantages for replication. The estimated frequency of HIV-1 viral strains with insertions in PR is 0.1%.36 We suspect a subtype bias favoring this type of PR insertions, as the frequency in our study is about 10 times higher and both strains are CRF02_AG. This observation was corroborated by scrutiny of the Los Alamos HIV database, where 12 (0.8%) of the 1463 CRF02_AG genomes listed contained amino acid insertions in PR.2
The observed absence of PI resistance mutations is concordant with the data from other studies in Africa.37,38 It also reflects the low usage of PIs in first-line therapy in Ghana. Subtype-related polymorphisms in the PR region, such as I13V, K20I, M36I, and H69K, could have an impact on PI susceptibility.17,25,39 Long-term assessment of patients infected with non-B viruses receiving PIs will be needed to establish the clinical impact of these polymorphisms. In this regard, it has been reported that the HIV-1 PR from 39 drug- naive Ghanaian patients were differentially less susceptible to PIs than subtype B samples, suggesting implications in the response to antiretroviral treatments including PIs.17
This is the largest study on genetic susceptibility of Ghanaian HIV-1 strains to ARV drugs. The phylogenetic analysis of PR-RT coding sequences also reveals useful data on the molecular epidemiology of HIV-1 in Ghana. In this genome segment, the frequency of URFs is 25%, although, for a real estimation, complete genome sequencing is required. The high frequency of URFs found in this study suggests a significant role for reinfections with diverse viral strains in West Africa. Therefore, in locations where different genetic forms cocirculate, it is useful to analyze possible intragene recombinations by bootscanning or other methods. Surveillance of HIV-1 subtypes may have important implications for vaccine development efforts in West Africa.40 These data highlight the need to promote more genetic studies on HIV in Africa, and both tasks, resistance and molecular epidemiology studies, could be simplified, compared with most published studies, by analyzing intragene recombination in PR-RT coding regions. Taken together these results, we can conclude that monitoring of HIV-1 drug resistance might provide data on the implications of intersubtype recombination in response to ART.41
We thank Dr R. Asare and all the laboratory staff of Atua Government Hospital, St Martin's Hospital, and Korle Bu Hospital for blood sample collection. The expert technical inputs of Simeon Aidoo, Justice Kumi, and Ivy Asante in the sample processing are very much appreciated. The valuable work of Yussif Ahmed Abdul Rahman in establishment of the patient database and data retrieval is commended. We kindly appreciate the expert contribution to sample processing of Mercedes Muñoz, Yolanda Vega, Elena Vázquez de Parga, Rocío Carmona, and Gema Casado. We thank the Genomic Unit at Centro Nacional de Microbiología, Instituto de Salud Carlos III (Spain), for technical assistance in sequencing.
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