(Niel) Malan, D. R MB, ChB*; Krantz, Edrich MB, ChB†; David, Neal MB, BCh‡; Wirtz, Victoria MS§; Hammond, Janet MD, PhD§; McGrath, Donnie MD§ ; for the 089 Study Group
Several potent ritonavir (RTV)-boosted protease inhibitor (PI)-based antiretroviral (ARV) regimens are recommended as preferred agents in guidelines for the initiation of treatment of HIV disease in ARV-naive patients, including atazanavir (ATV), the first once-daily PI.1,2 RTV boosting of PIs results in improved pharmacokinetics and may improve ARV activity and decrease the risk of development of drug resistance but also increases the risk of PI-related toxicities such as dyslipidemia, fat misdistribution, and insulin resistance.1
Unboosted ATV is recommended as an alternative PI for the initiation of treatment in ARV-naive patients.1 A previous registrational study in ARV-naive patients showed that ATV (400 mg once daily) is comparable in efficacy to efavirenz, an established standard of care for initial therapy, when both are given in combination with fixed-dose zidovudine-lamivudine.3 Results from an extended use rollover/switch study demonstrated that ATV did not result in significant increases in total cholesterol, fasting low-density lipoprotein (LDL) cholesterol, non-high-density lipoprotein (HDL) cholesterol, or triglyceride levels.4 Other studies also indicated that highly active antiretroviral therapy (HAART) containing unboosted ATV5-7 may mitigate the elevations in total cholesterol, fasting LDL cholesterol, and fasting triglycerides typically associated with other PI-based HAART regimens.8-10 A randomized trial with ARV-experienced patients demonstrated that 300 mg of ATV with 100 mg of ritonavir (ATV300/RTV) resulted in better lipid profiles compared with 400 mg of lopinavir (LPV) with RTV.11 The impact of the addition of RTV to ATV-based HAART on metabolic parameters is not well characterized among ARV-naive individuals, however. Consequently, there is a need for prospective data evaluating the tradeoffs represented by a potential increase in ARV activity weighed against possible increases in toxicity attributable to RTV boosting of ATV in ARV-naive patients compared with unboosted ATV.
This study was conducted to evaluate the efficacy, safety, and tolerability of a once-daily HAART regimen of ATV, with or without RTV, in ARV-naive patients at 48 and 96 weeks. Results through 48 weeks are presented in this report.
The study was conducted at 30 centers in 10 countries in North and South America, Africa, and Europe. HIV-infected adult men and women with a screening plasma HIV RNA level ≥2000 copies/mL (no CD4 cell count restrictions) were eligible for enrollment. Patients were excluded if they had previously received >30 days of nucleoside reverse transcriptase inhibitor (NRTI) therapy, >7 days of nonnucleoside reverse transcriptase inhibitor (NNRTI) or PI therapy, or any ARV therapy within 30 days of screening. Patients were randomized to once-daily treatment with ATV300/RTV or ATV at a dose of 400 mg (ATV400). Both groups also received 300 mg of lamivudine once daily and an investigational extended-release formulation of stavudine once daily (dosage adjusted for patient weight). Extended-release stavudine is not commercially available.
The study was approved by institutional review/ethics boards at all participating centers, and each participant provided signed informed consent before enrollment in the study.
Study Design and Procedures
This was a randomized, open-label, 2-arm study designed to demonstrate noninferiority of ATV300/RTV to ATV400. The primary endpoint was a comparison of the proportion of patients with an HIV RNA load <400 copies/mL at 48 weeks.
Participants were assessed at baseline and at weeks 2, 4, 8, 12, 16, 24, 32, 40, and 48. At each visit, participants were evaluated for toxicities and serum was collected for immunologic, virologic, resistance, chemistry, and hematology testing.
HIV RNA was measured using the AMPLICOR HIV-1 MONITOR test, version 1.5 (Roche Diagnostics, Pleasanton, CA). HIV isolates were tested for phenotypic resistance using the PhenoSense assay (Monogram Biosciences, South San Francisco, CA); substitutions to HIV reverse transcriptase and protease genomes were determined using the GenoSure assay (LabCorp of America, Burlington, NC). Resistance data for virologic failures were generated in real time and were made available to investigators for assistance in subsequent treatment decisions.
Safety assessments included reported adverse events (AEs); serious AEs (SAEs); discontinuations attributable to AEs; measured laboratory abnormalities; and changes from baseline in total cholesterol, fasting triglycerides, fasting LDL cholesterol, and HDL cholesterol.
A sample size of 200 patients (100 per group) provided at least 80% power to demonstrate noninferiority of ATV300/RTV to ATV400 as measured by the proportion of patients with an HIV RNA load <400 copies/mL at week 48. Noninferiority was considered to have occurred if the lower 95% confidence limit for the difference in proportions of responders in the ATV 300/RTV group versus ATV400 group was >−10%. The primary analysis was based on randomized intent-to-treat (ITT) patients using a time-to-loss-of-virologic-response (TLOVR) algorithm.12 Randomization was stratified by qualifying HIV RNA viral load (<100,000 copies/mL or ≥100,000 copies/mL). Identical statistical methods were used for the secondary endpoint (HIV RNA load <50 copies/mL). Treatment-emergent genotypic and phenotypic resistance profiles were assessed for patients with virologic failure through week 48.
The frequency of AEs, SAEs, death, laboratory abnormalities, and discontinuations attributable to AEs, and absolute and mean percent changes from baseline in serum lipids, were summarized through week 48. Differences in lipids between the groups were compared using 95% confidence intervals based on a t test stratified by qualifying HIV RNA. Data obtained after initiation of lipid-lowering therapy were excluded from all lipid analyses.
Baseline Characteristics and Patient Disposition
A total of 200 patients were enrolled and randomized: 95 to ATV300/RTV and 105 to ATV400. Median age, gender, and racial characteristics were balanced between treatment regimens (Table 1). At baseline, patients had a median viral load of approximately 5 log10 copies/mL and a median CD4 count of approximately 200 cells/mm3 (see Table 1). Twenty-one patients discontinued study therapy before week 48 (Table 2). The most commonly reported causes of treatment discontinuations before week 48 were AEs in the ATV300/RTV group (8%) and loss to follow-up in the ATV400 group (4%).
Virologic response rates by TLOVR analysis (viral load <400 copies/mL) at week 48 were 86% and 85% in the ATV300/RTV and ATV400 groups, respectively; virologic response rates using the more stringent endpoint of HIV RNA level <50 copies/mL were 75% and 70%, respectively (see Table 2; Fig. 1). ATV300/RTV met the criteria for noninferiority to ATV400 in both response categories.
Virologic response was comparable between treatment regimens when stratified by qualifying HIV RNA level (Fig. 2). Among participants with qualifying HIV viral loads <100,000 copies/mL, the proportions of responders with an HIV RNA load <400 copies/mL per the TLOVR algorithm were 91% and 92% for ATV300/RTV and ATV400, respectively (87% and 82% for HIV RNA load <50 copies/mL). Among participants with qualifying HIV viral loads ≥100,000 copies/mL, 82% and 78% in the respective treatment regimens responded per TLOVR analysis at an HIV RNA load <400 copies/mL (63% and 58%, respectively, at HIV RNA load <50 copies/mL). The median CD4 cell count increase from baseline through week 48 was comparable, at 174 cells/mm3 and 213 cells/mm3 in the ATV300/RTV and ATV400 groups, respectively (see Table 2).
Genotypic and Phenotypic Resistance
Criteria for virologic failure were met by 3 ATV300/RTV-treated patients and 10 ATV400-treated patients. Paired baseline and on-study HIV samples from 2 and 8 patients, respectively, were tested successfully for phenotypic and genotypic resistance and are shown in Table 3. Four of the 8 ATV400-treated patients with virologic failure had International AIDS Society (IAS)-USA Panel-defined minor PI-related substitutions, as did 1 ATV300/RTV-treated subject.13 Major substitutions emerged in 3 ATV400-treated patients: 2 had a mixed I50I/L substitution, and the third had pure I50L and N88S substitutions. The latter patient's isolate was the only one that demonstrated phenotypic resistance to ATV; it also demonstrated increased susceptibility to saquinavir, RTV, LPV, indinavir, and amprenavir compared with the baseline isolate. No emergence of IAS-USA Panel-defined major PI substitutions was observed in ATV300/RTV-treated patients.13 Major treatment-emergent NRTI substitutions were seen in 7 patients in the ATV400 group (all M184V) and in 1 in the ATV300/RTV group (M184M/V) (see Table 3).
Thirty-one subjects reported at least 1 SAE: 15% in the ATV300/RTV arm and 16% in the ATV400 arm (Table 4). Overall, only 2 of 38 SAEs were considered possibly related to treatment (depression and oculogyric crisis), and both occurred in the ATV400 arm. No individual SAE was reported by more than 2 patients.
Eight ATV300/RTV-treated patients and 1 ATV400-treated patient discontinued therapy because of AEs; 4 ATV300/RTV-treated patients discontinued by protocol mandate for persistent grade 4 total bilirubin elevations (defined as ≥2 elevations >5 times the upper limit of normal [ULN] within a 30-day period). Forty-three percent and 34% of patients on ATV300/RTV and ATV400, respectively, reported at least 1 grade 2 to 4 treatment-related AE. The most frequent (≥3%) grade 2 to 4 treatment-related AEs were jaundice, headache, and rash (see Table 4).
Grade 2 to 4 treatment-related jaundice occurred in 3% and <1% of ATV300/RTV- and ATV400-treated patients, respectively. Grade 3 to 4 jaundice (treatment-related) occurred in 1 patient on ATV300/RTV.
The rates of any grade of laboratory abnormalities were comparable between the 2 treatment regimens and were consistent with those previously reported.3,14 In addition, the rate of clinically relevant grade 3 to 4 laboratory abnormalities was low (≤6%) on both regimens, with the exception of hyperbilirubinemia (see Table 4). Grade 3 to 4 hyperbilirubinemia was more frequent among ATV300/RTV-treated patients (59%) than among ATV400-treated patients (20%). Three percent of patients in each group experienced a grade 3 to 4 hyperbilirubinemia and a grade 3 to 4 elevation in liver enzymes (alanine aminotransferase and/or aspartate aminotransferase).
ATV300/RTV-treated patients had greater changes in all lipid parameters than did ATV400-treated patients (Table 5). Cholesterol and triglycerides were examined by National Cholesterol Education Program (NCEP) categories.15 The proportions of patients with upward shifts of at least 1 NCEP category at week 48 were comparable between the ATV300/RTV and ATV400 regimens for total cholesterol (16% and 11%) and fasting LDL cholesterol (46% and 48%), respectively. There were more patients in the ATV300/RTV group (30%) than in the ATV400 group (18%) with upward shifts of at least 1 NCEP category in fasting triglycerides. The proportions of patients with HDL cholesterol levels ≥40 mg/dL (1 mmol/L) in the ATV300/RTV and ATV400 regimens were similar at baseline (41% and 35%) and at week 48 (71% and 71%), respectively. The proportions of patients with baseline total-to-HDL cholesterol ratios >5 were 35% in the ATV300/RTV group and 38% in the ATV400 group. At week 48, the percentages decreased to 24% and 14% in the ATV300/RTV and ATV400 groups, respectively. Lipid-lowering agents were used on study by 4% of ATV300/RTV-treated patients and 3% of ATV400-treated patients.
Triple HAART containing a PI and 2 NRTIs has resulted in dramatic decreases in HIV-1-related morbidity and mortality. Three RTV-boosted PIs-ATV/RTV once daily, fosamprenavir/RTV (FPV/RTV) twice daily, and LPV/RTV twice daily-are currently recommended for the initial treatment of patients infected with HIV-1.2 FPV/RTV and LPV/RTV were compared in a large randomized controlled trial in ARV-naive patients and were found to have similar efficacy and safety through 48 weeks.10 Acknowledging the smaller size of the current study, and that there are limitations to cross-study comparisons, the response rates at the threshold of <50 copies/mL at week 48 in the current study of 75% for RTV-boosted ATV and 70% for unboosted ATV400 were comparable to those observed for FPV/RTV (66%) and LPV/RTV (65%).10
A finding of note in the current study was that response rates on both regimens varied depending on patients' qualifying HIV RNA levels. This extent of variation in response rates has not been observed in studies of other boosted PIs.10,16 For patients with viral loads ≥100,000 copies/mL on entry into the current study, the response rates at an HIV RNA level <50 copies/mL for ATV300/RTV and ATV400 were 63% and 58%, respectively. These rates are in the same range as those previously reported for LPV/RTV- and FPV/RTV-based HAART regimens among patients with baseline HIV RNA levels of ≥100,000 copies/mL or <100,000 copies/mL.10 In contrast, ATV300/RTV and ATV400 achieved greater response rates among those patients with qualifying HIV RNA levels <100,000 copies/mL, where response rates at an HIV RNA level <50 copies/mL were 87% and 82%, respectively. The mechanism for these differential response rates remains to be elucidated. A large, ongoing, international, randomized controlled trial comparing ATV300/RTV and LPV/RTV in ARV-naive patients (AI424-138) may provide further understanding of virologic response rates in all subsets of patients, including those with high baseline HIV-1 RNA levels (≥100,000 copies/mL) and those with low baseline CD4 counts (<50 cells/mm3).
Virologic failure occurred infrequently with ATV300/RTV (3 patients). This is consistent with reports of failure with other boosted PIs in ARV-naive patients from clinical trials and clinical reports.10,17-19 This further reinforces the notion that virologic failure occurs infrequently with boosted-PI-based HAART in ARV-naive patients. In contrast to ATV300/RTV, 10% of patients on ATV400 experienced virologic failure by 48 weeks. Of the 8 patients with virologic failure and typable on-study isolates, 3 had IAS-USA Panel-defined major PI substitutions. Several patients with virologic failure on ATV400 also had a detectable M184V substitution, associated with lamivudine resistance. In contrast, only 1 patient with virologic failure on ATV300/RTV developed an isolate with the mixed M184M/V substitution. The small difference in virologic failure rates and the different emergent resistance patterns likely reflect the higher ATV plasma concentrations that are achieved with RTV boosting.
Tolerability and safety and the numbers of overall treatment discontinuations were similar between the groups. The rates of grade 2 to 4 AEs and SAEs were consistent with those previously reported with ATV treatment.3 More patients on ATV300/RTV (8%) than on ATV400 (<1%) discontinued treatment because of AEs. More than half of the ATV300/RTV-treated patients who discontinued because of AEs did so by protocol mandate. These patients were required to discontinue study therapy in cases of persistent grade 4 hyperbilirubinemia irrespective of clinical symptoms. The higher rates of hyperbilirubinemia observed in the ATV300/RTV arm were likely attributable to RTV-induced increases in ATV concentrations.20 Mandated discontinuation of ATV attributable to persistent grade 4 hyperbilirubinemia does not mirror current clinical practice, in which ATV discontinuation for hyperbilirubinemia is typically recommended only when clinically evident jaundice presents a cosmetic concern to the patient. In a recent study of treatment-naive subjects treated with boosted-PI regimens, discontinuation rates at 48 weeks were 21% and 22% for FPV/RTV and LPV/RTV, respectively.10 In contrast, among treatment-naive patients receiving ATV/RTV, discontinuation rates of 12% through 48 weeks in the current study may reflect the known tolerability of ATV-based regimens in this population.3
A major concern for PI-based HAART is the development of metabolic toxicity, including dyslipidemias,21 which may contribute to increased long-term cardiovascular risk.22-24 In ARV-naive patients started on HAART, some, if not all, of the lipid changes seen in the first 48 weeks of therapy may be attributable to a “return-to-health effect”.25 In this study, both treatment regimens demonstrated increases from baseline in total cholesterol, fasting LDL cholesterol, and HDL cholesterol; fasting triglycerides increased from baseline only in the boosted arm. The increases in total cholesterol, fasting LDL cholesterol, and triglycerides observed in the current study were lower than those recently reported for the other boosted PIs recommended for ARV-naive patients.10 From a clinical perspective, it is important to note that few patients in this study changed NCEP categories for total cholesterol by week 48 and that the percentage of subjects with total cholesterol/HDL cholesterol ratios >5 decreased on both regimens by week 48.
The increases in total cholesterol and fasting triglycerides in this study were greater in patients receiving ATV300/RTV, suggesting that boosting with RTV may have some minimal impact on the lipid advantage of ATV; however, the changes seem to be of less magnitude than those seen with other boosted PIs.10
The increases from baseline in lipid parameters in the ATV400 arm in this study were higher than the changes seen in previous studies of unboosted ATV in ARV-naive patients.5,26 Some of the increases observed in the lipid parameters may be at least partially explained by the known impact of stavudine on lipid profiles,27,28 although the changes are greater than observed in previous studies of ATV400 used in combination with stavudine in ARV-naive subjects.26
It is important to note the limitations of this study, such as the open-label design, which may result in biased responses in safety assessments and reporting. It is unlikely, however, given the low discontinuation rate, that this study design would affect virologic, immunologic, or resistance endpoints significantly.
Several findings in this study suggest that boosted ATV may be more potent than unboosted ATV in treatment-naive subjects; these include the greater TLOVR response rates at the most stringent endpoint of an HIV RNA load <50 copies/mL, the lower rates of virologic failure, the absence of major PI substitutions, and the rarity of the M184V substitution seen in subjects with virologic failure on ATV300/RTV. As noted, however, there was not a statistically significant difference seen in any efficacy endpoint. The results do nonetheless provide useful information for clinicians to consider when selecting ATV300/RTV, LPV/RTV, and FPV/RTV, the 3 RTV-boosted PIs that are currently recommended for initial treatment of ARV-naive patients.1,2
Factors that affect selection of first-line ARV therapy are many, and include efficacy, tolerability, the need to preserve future treatment options, and, in some areas of the world, the availability of refrigeration for the storage of RTV. In addition, drug-drug interactions may have an impact on the choice of agents used in first-line ARV therapy. For instance, unboosted ATV cannot be coadministered with the NRTI tenofovir disoproxil fumarate (DF) because of reduced levels of ATV, and RTV boosting is required in this instance.1,2 This novel head-to-head study comparing 2 different modes of ATV administration demonstrates that unboosted ATV remains a viable option for ARV-naive patients and may be of particular use for patients who are intolerant of or experience toxicity from low-dose RTV, or in areas of the world where patients do not have access to adequate refrigerated storage for RTV. The unique resistance profile of ATV also may preserve the option of future treatment with other PIs.
In conclusion, these findings demonstrate the safety and efficacy of the ATV300/RTV regimen and confirm the safety and efficacy of ATV400 in an ARV-naive patient population. Further studies to assess the safety, efficacy, and metabolic effects of ATV300/RTV compared with a second standard-of-care boosted PI should better characterize the use of ATV with RTV in HIV-infected ARV-naive patients.
The authors are grateful to the many persons with HIV infection who volunteered for this study.
The authors thank the following collaborators: Sujata Lalla-Reddy (Ocean View Internal Medicine, Long Beach, CA), G. Steven Kooshian (Ocean View Internal Medicine, Long Beach, CA), Javier Morales Ramirez (Clinical Research Pr Inc, San Juan, PR), Juan Echevarria (Hospital Nacional Cayetano Heredia, Lima, Peru), Raul Salazar (Hospital Nacional Guillermo Almenara Irigoyen, Lima, Peru), Juan Ballesteros (Hospital Del Salvador, Santiago De Chile, Metropolitana Chile), Carlos Beltran (Hospital Barros Luco, Santiago De Chile, Metropolitana Chile), Eduardo Bargman (Hospital Rivadavia, Buenos Aires, Argentina), Marcelo Martins (Centro Privado Tomografia Computada Cordoba SA, Cordoba, Argentina), Liliana Puga (Centro De Infectologia Y Asistencia En Sida, Buenos Aires, Argentina), Javier Altclas (Sanatorio Mitre, Buenos Aires, Argentina), Jorge Contarelli (Hospital San Juan De Dios De La Plata, Buenos Aires, Argentina), Marek Beniowski (Szpital Specjalistyczny, Chorzow, Poland), Waldemar Halota (Klinika Chorob Zakaznych Am, Bydgoszcz, Poland), Andrzej Horban (Wojewodzki Szpital Zakazny, Warszawa, Poland), Vadim Pokrovsky (Central Research Institute of Epidemiology of Ministry of Public Health RF, Moscow, Russia), Boris Gruzdev (Municipal Infectious Hospital #2, City AIDS Center, Moscow, Russia), Alexey Yakovlev (City Infectious Hospital #30, St. Petersburg, Russia), Fernando Mendo (Hospital Nacional Edgardo Rebagliati Martins, Lima, Peru), Robert Flisiak (Klinika Obserwacyjno-Zakazna, Bialystok, Poland), and Juan Carlos Tinoco (Hospital General De Durango, Durango, Mexico).
The roles of the authors included study conduct, review and presentation of results, writing, and review of the manuscript. The corresponding author had the final responsibility for manuscript submission. The authors gratefully acknowledge the assistance and provision of summary data from Bristol-Myers Squibb. Stacey Shehin, PhD (i3 Statprobe, Ann Arbor, MI) assisted in preparation and editing of this article.
1. Hammer SM, Saag MS, Schechter M, et al. Treatment for adult HIV infection: 2006 recommendations of the International AIDS Society-USA Panel. JAMA
3. Squires K, Lazzarin A, Gatell JM, et al. Comparison of once-daily atazanavir with efavirenz, each in combination with fixed-dose zidovudine and lamivudine, as initial therapy for patients infected with HIV. J Acquir Immune Defic Syndr
4. Wood R, Phanuphak P, Cahn P, et al. Long-term efficacy and safety of atazanavir with stavudine and lamivudine in patients previously treated with nelfinavir or atazanavir. J Acquir Immune Defic Syndr
5. Jemsek JG, Arathoon E, Arlotti M, et al. Body fat and other metabolic effects of atazanavir and efavirenz, each administered in combination with zidovudine plus lamivudine, in antiretroviral-naive HIV-infected patients. Clin Infect Dis
6. Sension M, Grinsztejn B, Molina J, et al. A1424067: improvement in lipid profiles after 12 weeks of switching to atazanavir from boosted or unboosted protease inhibitors in patients with no previous PI virologic failure and hyperlipidemia at baseline [abstract 858]. Presented at: 12th Conference on Retroviruses and Opportunistic Infections; 2005; Boston.
7. Gatell J, Salmon-Ceron D, Lazzarin A, et al. Efficacy and safety of atazanavir-based highly active antiretroviral therapy in patients with virologic suppression switched from a stable, boosted or unboosted protease inhibitor treatment regimen: the SWAN Study (AI424-097) 48-week results. Clin Infect Dis
8. Carr A, Samaras K, Burton S, et al. A syndrome of peripheral lipodystrophy, hyperlipidaemia and insulin resistance in patients receiving HIV protease inhibitors. AIDS
9. Levy AR, McCandless L, Harrigan PR, et al. Changes in lipids over twelve months after initiating protease inhibitor therapy among persons treated for HIV/AIDS. Lipids Health Dis
10. Eron J Jr, Yeni P, Gathe J Jr, et al. The KLEAN study of fosamprenavir-ritonavir versus lopinavir-ritonavir, each in combination with abacavir-lamivudine, for initial treatment of HIV infection over 48 weeks: a randomised non-inferiority trial. Lancet
11. Johnson M, Grinsztejn B, Rodriguez C, et al. Atazanavir plus ritonavir or saquinavir, and lopinavir/ritonavir in patients experiencing multiple virological failures. AIDS
12. U.S. Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research. Guidance for industry: antiretroviral drugs using plasma HIV RNA measurements-clinical considerations for accelerated and traditional approval. Available at: http://www.fda.gov/cder/guidance/3647fnl.htm
. Accessed March 28, 2007.
13. Johnson VA, Brun-Vézinet F, Clotet B, et al. Update of the drug resistance mutations in HIV-1: fall 2006. Top HIV Med
14. Sanne I, Piliero P, Squires K, et al. Results of a phase 2 clinical trial at 48 weeks (AI424-007): a dose-ranging, safety, and efficacy comparative trial of atazanavir at 3 doses in combination with didanosine and stavudine in antiretroviral-naive subjects. J Acquir Immune Defic Syndr
15. Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults. Executive summary of the Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). JAMA
17. Conradie F, Sanne I, Venter W, et al. Failure of lopinavir-ritonavir (Kaletra)-containing regimen in an antiretroviral-naive patient. AIDS
18. Friend J, Parkin N, Liegler T, et al. Isolated lopinavir resistance after virological rebound of a ritonavir/lopinavir-based regimen. AIDS
19. Sax PE, Xu F, Tisdale M, et al. First report of development of resistance to boosted fosamprenavir in an ART-naïve subject: virologic and clinical outcome [abstract H-1060]. Presented at: 45th Interscience Conference on Antimicrobial Agents and Chemotherapy; 2005; Washington.
20. Agarwala S, Eley T, Filoramo D. Steady-state pharmacokinetics and inhibitory quotient of atazanavir with and without ritonavir in treatment-naive HIV-infected patients [abstract 85]. Presented at: Seventh International Workshop on Clinical Pharmacology of HIV Therapy; 2006; Lisbon.
21. Hadigan C, Meigs JB, Corcoran C, et al. Metabolic abnormalities and cardiovascular disease risk factors in adults with human immunodeficiency virus infection and lipodystrophy. Clin Infect Dis
22. Mary-Krause M, Cotte L, Simon A, et al. Increased risk of myocardial infarction with duration of protease inhibitor therapy in HIV-infected men. AIDS
23. Friis-Møller N, Sabin CA, Weber R, et al. Combination antiretroviral therapy and the risk of myocardial infarction. N Engl J Med
24. Klein D, Hurley L, Quesenberry C, et al. Hospitalizations for CHD and MI among Northern California HIV+
men: changes in practice and Framingham risk scores [abstract 737]. Presented at: 13th Conference on Retroviruses and Opportunistic Infections; 2006; Denver.
25. van Leeuwen R, Katlama C, Murphy RL, et al. A randomized trial to study first-line combination therapy with or without a protease inhibitor in HIV-1-infected patients. AIDS
26. Murphy RL, Sanne I, Cahn P, et al. Dose-ranging, randomized, clinical trial of atazanavir with lamivudine and stavudine in antiretroviral-naive subjects: 48-week results. AIDS
27. Gallant JE, Staszewski S, Pozniak AL, et al. Efficacy and safety of tenofovir DF vs stavudine in combination therapy in antiretroviral-naive patients: a 3-year randomized trial. JAMA
28. Moyle GJ, Baldwin C, Langroudi B, et al. A 48-week, randomized, open-label comparison of three abacavir-based substitution approaches in the management of dyslipidemia and peripheral lipoatrophy. J Acquir Immune Defic Syndr
© 2008 Lippincott Williams & Wilkins, Inc.