JAIDS Journal of Acquired Immune Deficiency Syndromes:
Epidemiology and Social Science
Mortality and Morbidity Among Postpartum HIV-Positive and HIV-Negative Women in Zimbabwe: Risk Factors, Causes, and Impact of Single-Dose Postpartum Vitamin A Supplementation
Zvandasara, Partson FCOG (SA)*†; Hargrove, John W. PhD†; Ntozini, Robert BSc†; Chidawanyika, Henry BSc†; Mutasa, Kuda BSc†; Iliff, Peter J. MD†‡; Moulton, Lawrence H. PhD†§; Mzengeza, Faith BSc†∥; Malaba, Lucie C. PhD†∥; Ward, Brian J. MD†¶; Nathoo, Kusum J. MRCP†‡; Zijenah, Lynn S. PhD†#; Mbizvo, Michael PhD†††; Zunguza, Clare MSc†**; Humphrey, Jean H. ScD†§; The ZVITAMBO Study Group
From the *Department of Obstetrics and Gynaecology, University of Zimbabwe, College of Health Sciences, Harare, Zimbabwe; †ZVITAMBO Project, Harare, Zimbabwe; ‡Department of Paediatrics and Child Health, University of Zimbabwe, College of Health Sciences; ∥College of Science, Institute of Nutrition and Family Sciences, Harare, Zimbabwe; ¶The Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada; §The Johns Hopkins Bloomberg School of Public Health, Department of International Health, Baltimore, MD; #Department of Immunology, University of Zimbabwe, College of Health Sciences; **Harare City Health Department, Harare, Zimbabwe; and ††WHO, Switzerland.
Received for publication November 9, 2004; accepted May 16, 2006.
The ZVITAMBO project was supported by the Canadian International Development Agency (CIDA) (R/C Project 690/M3688), United States Agency for International Development (USAID) (cooperative agreement number HRN-A-00-97-00015-00 between The Johns Hopkins University and the Office of Health and Nutrition-USAID), and a grant from the Bill and Melinda Gates Foundation, Seattle WA. Additional funding was received from the Rockefeller Foundation (New York, NY) and BASF (Ludwigshafen, Germany).
Other members of the ZVITAMBO Study Group, in addition to the named authors, are Agnes Mahomva, Florence Majo, Edmore Marinda, Mary Ndhlovu, Ellen Piwoz, Lidia Propper, Phillipa Rambanepasi, Andrea Ruff, and Naume Tavengwa.
Reprints will not be available from the authors.
Correspondence to: Jean H. Humphrey, ScD, ZVITAMBO Project, 1 Borrowdale Road, Borrowdale, Harare, Zimbabwe (e-mail: email@example.com).
Background: Vitamin A deficiency is common among women in resource-poor countries and is associated with greater mortality during HIV.
Methods: Fourteen thousand one hundred ten mothers were tested for HIV and randomly administered 400,000 IU vitamin A or placebo at less than 96 hours postpartum. The effects of vitamin A and HIV status on mortality, health care utilization, and serum retinol were evaluated.
Results: Four thousand four hundred ninety-five (31.9%) mothers tested HIV positive. Mortality at 24 months was 2.3 per 1000 person-years and 38.3 per 1000 person-years in HIV-negative and HIV-positive women, respectively. Vitamin A had no effect on mortality. Tuberculosis was the most common cause of death, and nearly all tuberculosis-associated deaths were among HIV-positive women. Among HIV-positive women, vitamin A had no effect on rates of hospitalization or overall sick clinic visits, but did reduce clinic visits for malaria, cracked and bleeding nipples, pelvic inflammatory disease, and vaginal infection. Among HIV-negative women, serum retinol was responsive to vitamin A, but low serum retinol was rare. Among HIV-positive women, serum retinol was largely unresponsive to vitamin A, and regardless of treatment group, the entire serum retinol distribution was shifted 25% less than that of HIV-negative women 6 weeks after dosing.
Conclusions: Single-dose postpartum vitamin A supplementation had no effect on maternal mortality, perhaps because vitamin A status was adequate in HIV-negative women and apparently unresponsive to supplementation in HIV-positive women.
Vitamin A deficiency is common among women of reproductive age in resource-poor countries1 and is associated with increased risk of morbidity2 and mortality.3 In a trial among nearly 45,000 women in Nepal, weekly vitamin A supplementation reduced maternal mortality by 40%.4 Vitamin A deficiency is also common during HIV infection and is an independent risk factor for associated mortality.5,6 In Africa, HIV is the chief cause of adult mortality7 and substantially contributes to maternal mortality.8-10 Specifically, between 1994 and 1999 in Zimbabwe, when HIV prevalence among antenatal women escalated from 3% to 25%, total adult female mortality, maternal mortality rate, and maternal mortality ratio all more than doubled, even as fertility declined.11
The World Health Organization (WHO) recommends universal distribution of a single 200,000-IU dose of vitamin A to women during the postnatal infertile period in places where deficiency is a public health problem.12 Single-dose postpartum maternal dosing is currently implemented in at least 15 countries, including several where HIV is also prevalent. The intervention improves vitamin A status,13,14 but its impact on mortality and morbidity has not been investigated among either HIV-infected or uninfected women. Moreover, because 200,000 IU is probably too little to replete deficient women,13 doubling this regimen to two 200,000-IU doses is under consideration.15,16
We investigated the effects of a single, high-dose, postpartum vitamin A supplement on serum retinol concentration, and total and cause-specific mortality and morbidity among HIV-positive and HIV-negative postpartum women enrolled in the Zimbabwe Vitamin A for Mothers and Babies (ZVITAMBO) project. Zimbabwe is categorized by WHO as "high risk" for vitamin A deficiency.12 At the time of the study, there was not a national vitamin A supplementation program for postpartum women, although it was initiated approximately 18 months after recruitment ended based on findings from a national micronutrient survey.17
The ZVITAMBO protocol has been previously described.18-20 Briefly, between November 1997 and January 2000, 14,110 mothers and their neonates were enrolled within 96 hours of delivery from 14 maternity clinics and hospitals in greater Harare. Women were randomly administered 400,000 IU vitamin A (orally as retinyl palmitate) or placebo. Mother-baby pairs were excluded if either had an acute life-threatening condition, the baby was from a multiple birth or had birth weight of less than 1500 g, or they resided outside Harare. Written informed consent was obtained.
At recruitment, women were tested for HIV by 2 enzyme-linked immunosorbent assays run in parallel (HIV 1.0.2 ICE; Murex Diagnostics, Edenvale, South Africa; and GeneScreen HIV 1/2; Sanofi Diagnostics Pasteur, Johannesburg, South Africa). Duplicate pairs of discordant enzyme-linked immunosorbent assay test results were resolved by Western blot (HIV Blot 2.2; Genelabs Diagnostics SA, Geneva, Switzerland). Hemoglobin (Hb) was measured for women enrolled from October 1998 to the end of the study (approximately 60% of the total sample) by HemoCue (Mission Viejo, CA).21 Serum retinol concentration was quantified by high-performance liquid chromatography (HPLC)20 for representative subsamples of 398 HIV-negative and 691 HIV-positive women. Among HIV-positive mothers, CD4 cells were enumerated (Facscount; Becton Dickinson International, Erembodegem, Belgium), and viral load was measured in a representative subsample of 444 women (Roche Amplicor HIV-1 Monitor test version 1.5; Roche Diagnostics, Alameda, CA). Mid-upper arm circumference (MUAC) was measured.22 Baseline characteristics were collected by questionnaire and transcription from hospital records.
Mothers were followed up at 6 weeks, 3 months, and then 3 monthly up to 12 to 24 months. Women testing HIV positive at baseline were retested at their next blood draw; HIV-negative women were retested at every subsequent blood draw. We initially planned to follow up all HIV-positive mothers and a randomly selected 50% of HIV-negative women for 24 months. However, in June 2000, economic conditions necessitated discontinuing the second year of follow-up. This meant that 24%, 48%, and 100% of the women were reassigned to 24-month, at least 18-month, and more than 12-month follow-ups, respectively. Medical care and counseling20,23,24 were offered, and travel costs were reimbursed throughout the trial.
Morbidity was assessed by health care utilization.25 At each visit, women were asked if they had sought treatment of illness since the previous visit. Health care including an overnight stay was defined as hospitalization; outpatient care was defined as a clinic visit. Cause of health care visits was determined by review of the "ZVITAMBO Sick Visit Card," issued to all study participants at enrolment. Women presented this card to health care providers outside the study, who were invited to list all diagnoses made at the visit. Illness requiring treatment during a study visit was also recorded on this card. If information was not available from this card, cause of visit was determined from government health records or by the woman's report.
Cause of death was determined from medical records, if available, or by review of verbal autopsy by the study gynecologist (P.Z.), who was masked to treatment group and HIV status. Multiple causes were recorded and not ranked.26 Deaths due to suicide, murder, and accidents were assigned "injury" as cause.
Statistical analysis was conducted using SAS (v.8.2, Cary, NC) and STATA (v.8, College Station, TX). Baseline characteristics were compared by treatment group and baseline HIV status. Cumulative mortality of the 4 treatment-HIV status strata were estimated by Kaplan-Meier (K-M), and differences were tested by log-rank tests at 6 months (183 days), 12 months (365 days), and 24 months (731 days). Mortality rates with person-time denominators were also calculated. Cox models were used to investigate the adjusted effect of vitamin A supplementation on mortality at 12 months (maternal + late maternal mortality27) among all women and for HIV-positive and HIV-negative women separately. A forward stepwise selection procedure was used, using inclusion and retention levels of α = 0.20 and 0.05, respectively. The proportion of deaths in the study population attributable to HIV (population attributable fraction [PAF]) was calculated as [PAF% = Pd (1− 1/RR) × 100] where Pd is the proportion of deaths of HIV-positive women to all deaths, and RR is the risk of death in the HIV-positive/HIV-negative women.28
Risk ratios for cause-specific death rates were calculated for women who received vitamin A relative to placebo and for women who were HIV-positive relative to negative for the first and second years postpartum. The denominators for these rates were the number of women who died of the cause during the 12-month period plus the number known to survive the period.
Investigation of the effect of vitamin A on serious morbidity, as reflected by health care visits, focused on HIV-positive women. Incident rate ratios (IRRs) were calculated to compare total and cause-specific sick clinic visits during the postpartum year between treatment groups. A Poisson model was used for analyses in which all women included had only 1 visit; a quasi-likelihood Poisson model corrected for overdispersion was used for analyses that included at least 1 woman with more than 1 visit. This analysis was repeated adjusting for baseline CD4. The proportion of women ever hospitalized, the number of hospitalizations, and the days of hospitalization were compared across treatment groups. IRRs were calculated, with and without adjustment for CD4. Initial data exploration revealed that 82% of these hospitalizations were only 1 day in duration (i.e., only overnight observation was required), whereas the remaining ranged from 2 to 70 days' duration (i.e., reflecting serious illness). Therefore, duration of hospitalization was compared across treatment groups by logistic regression for the outcome 1 day versus more than 1 day.
Of the 14,110 mothers enrolled, 9562 (67.8%) tested HIV-negative at baseline and 4495 (31.9%) tested HIV-positive. The remaining 53 mothers (0.4%) tested HIV-indeterminate and were excluded from the analysis (Fig. 1).
Baseline characteristics did not significantly differ between treatment groups within either HIV stratum, except for a small imbalance in husband's education among HIV-negative women (Table 1). After collapsing treatment groups within HIV strata, HIV-positive women were older (25.6years [5.0 years] vs. 24.1 years [5.4 years]; P < 0.0001), of higher parity (2.3 [1.2] vs. 2.0 [1.3]; P < 0.0001), and more likely to be widowed or divorced compared with uninfected women. Median household incomes were similar for HIV-infected and uninfected mothers, but a larger proportion of infected women lived in the poorest households (22% of positive compared with 16% of negative women had total daily household income of <US$1.20, P < 0.0001). MUAC, CD4, and Hb concentrations were, expectedly, lower in HIV-positive compared with HIV-negative women.
At baseline, mean [SD] serum retinol concentration was higher for HIV-negative than HIV-positive women (1.18 μmol/L [0.39 μmol/L] vs 0.98 μmol/L [0.35 μmol/L]; P < 0.0001) and a greater proportion of positive compared with negative women had concentrations of less than 1.05 μmol/L, the conventional cutoff for deficiency in women (60.8% vs 37.4%; P < 0.0001) (Table 2). At 6 weeks, serum retinol concentrations increased in both treatment groups, reflecting recovery from pregnancy-associated hemodilution29 and colostrum production demands of parturition30 (Table 2). Among HIV-negative women, the mean change between baseline and 6 weeks was significantly greater for women in the vitamin A compared with placebo group, demonstrating a small treatment effect (Table 2). Among HIV-positive women with baseline CD4 count of less than 200 cells × 106/L, vitamin A supplementation significantly increased mean serum retinol and halved the proportion of women with concentrations of less than 1.05 μmol/L (from 60% to 30%). However, for all other HIV-positive women, supplementation had no effect on serum retinol concentration. Among women in the vitamin A group, the 6-week serum retinol distribution was shifted ∼30% higher for HIV-negative compared with HIV-positive women, even among those with more than 600 CD4 cells × 106/L, who were likely to have had "asymptomatic" HIV disease (Fig. 2).
Vitamin A supplementation had no effect on mortality of all women combined or for either HIV-status group (Fig. 3).
At 12 and 24 months, respectively, mortality rates among HIV-negative mothers were 0.27% (0.18-0.40, here and elsewhere ranges give 95% confidence limits; 24 deaths) and 0.38% (0.24.59; 3 deaths) by K-M survival analysis, and 2.7 per 1000 person-years (p-y) and 2.3 per 1000 p-y by person-time analysis. During the same periods, mortality rates for HIV-positive women were 3.6% (3.0-4.2; 149 deaths) and 7.7% (6.7-8.7; 88 deaths) by K-M survival analysis, and 36.5 per 1000 p-y and 38.3 per 1000 p-y by person-time analysis. Compared with HIV-negative women, HIV-positive women were 14.2 (8.9-22.6) and 54.1 (13.3-220) times more likely to die during the first and second postpartum years, respectively, after adjusting for age. During the first and second years, respectively, HIV-positive women made up 88% (143/163) and 98% (88/90) of all deaths (P = 0.007, χ2, df = 1 for the difference in the percentage between years), and the PAF of death caused by HIV was 82% and 96%. Among HIV-negative women, the conditional probability of death during the second 12 months, given survival to 12 months postpartum, was 100 × [(0.38 − 0.27)/(100 − 0.27)] = 0.11% (ie, less than half that of the first year). Conversely, among HIV-positive women, the corresponding probability was 100 × [(7.7 − 3.6)/(100 − 3.6)] = 4.3% (i.e., slightly higher in the second compared with the first year). In fully adjusted Cox analyses applied to data in 5-year age strata, the increased hazard of death associated with HIV was slightly lower among teenagers (12.9), rising to 17.1 among women aged 25 to 30 years, and then declining to 9.1 among women older than 40 years. However the 95% confidence intervals (CIs) of the estimates for each age group were wide (primarily due to the very small number of deaths among HIV-negative women), and there was no statistically significant effect of age (P = 0.06 and 0.08 for the linear and quadratic effects, respectively) (Fig. 4).
Risk Factors for Mortality
Among HIV-negative women, age, widowhood, and husband's education were independently predictive of mortality (data not presented). The adjusted hazard ratios of mortality associated with vitamin A supplementation at 6, 12, and 24 months were 1.4 (0.4-5.0), 1.11 (0.81, 1.51, and 1.0 (0.4-2.3), respectively.
Among HIV-positive women, age, parity, CD4 and Hb concentrations, MUAC, marital status, maternal education, and the survival of the previous child were independently predictive of mortality (Table 3). Parity and loss of the previous child entered jointly into the model: primiparous mothers and those who had lost their previous child were at higher risk than those with a living previous child. The adjusted hazard ratios of mortality associated with vitamin A treatment at 6, 12, and 24 months were 1.1 (0.7-1.7), 1.1 (0.8-1.6), and 1.0 (0.8-1.3), respectively.
Cause of Death
There was no significant effect of the vitamin A supplementation on any cause-specific risk of death among HIV-positive or HIV-negative women (data not presented).
Cause-specific mortality during the postpartum year was significantly higher among HIV-positive compared with HIV-negative women for all causes except hypertension and accident (Table 4). The risk ratios were highest for causes associated with HIV infection, but HIV-positive women were also significantly more likely to die due to obstetric causes: 88% (7/8) and 90% (9/10) of deaths associated with direct maternal causes within 42 days and 1 year postpartum, respectively, were among HIV-positive women.
Sick Clinic Visits and Hospitalizations Among HIV-Positive Women
HIV-positive women made a total of 8322 sick clinic visits during the postpartum year (Table 5). After correction for overdispersion, vitamin A supplementation had no overall effect on sick clinic visits (IRR, 0.95 [0.86-1.04]). However, vitamin A supplementation was associated with significant reductions in cause-specific clinic visits for malaria, vaginal infection, pelvic inflammatory disease, and cracked or bleeding nipples. Women with baseline CD4 count of less than 200 cells × 106/L had 1.8 (1.6-2.0) times more sick clinic visits compared with infected women with higher CD4 counts, but adjustment for CD4 did not alter the vitamin A effect (data not presented).
During the postpartum year, 259 HIV-positive women were hospitalized at least once (126 and 133 in the vitamin A and placebo groups, respectively), for 339 hospitalizations (165 [vitamin A] and 174 [placebo]), and 880 total hospital days (390 [vitamin A] and 490 [placebo]). Only 28 HIV-positive women in the vitamin A group and 34 in the placebo group had a hospitalization of more than 1 day's duration. After correction for overdispersion, there was no effect of vitamin A on the proportion of women ever hospitalized (IRR, 0.98 [0.76-1.26]), the number of hospitalizations (0.96 [0.66-1.40]), or the risk of having a hospitalization of more than 1 day in duration (0.84 [0.45-1.58]). HIV-positive women with CD4 of less than 200 cells were 3.0 (2.28-4.00) times more likely to be hospitalized and had 3.6 (2.4-5.3) times more hospitalizations than HIV-positive women with higher CD4 counts, but adjusting for CD4 had no effect on hazard ratios associated with vitamin A treatment.
A single 400,000-IU dose of vitamin A given during the immediate postpartum period had no effect on mortality among HIV-negative or HIV-positive women. Lack of effect among HIV-negative women might be because few were apparently vitamin A deficient: the distribution of serum retinol concentration at 6 weeks, even among those who received placebo, lay midway between those of similarly-aged white and black women in the United States National Health and Nutrition Examination Survey III.31
Among HIV-positive women, vitamin A deficiency was apparently common: 30% had a serum retinol concentration of less than 1.05 μmol/L at 6 weeks. Despite this, serum retinol increased in response to vitamin A supplementation only among the 15% of HIV-positive women who had CD4 count of less than 200 cells × 106/L. Among all other HIV-positive women, 30% remained with a concentration of less than 1.05 μmol/L regardless of their baseline CD4 count or whether they had received vitamin A, and the entire distribution was shifted substantially lower than that of HIV-negative women 6 weeks after supplementation. In short, the primary factor predicting serum retinol concentration 6 weeks after dosing was HIV status, not receipt of vitamin A or placebo.
The programmatic implications of this depend on whether these low serum retinol concentrations reflect deficiency that might have responded to higher or more continuously administered doses of vitamin A or an HIV-induced metabolic alteration intractable to even more aggressive supplementation. We believe that our data are more consistent with the second possibility. Retinol circulates in complex with 2 protein carriers, retinol-binding protein (RBP) and transthyretin (TTR),32 which are both "negative acute-phase proteins" (APPs). During the acute-phase response (APR) to infection, RBP, TTR, and retinol concentrations decline.33 However, among asymptomatic, HIV-positive men in the United Kingdom, plasma concentrations of RBP and TTR were not reduced compared with HIV-negative healthy controls, leading the authors to conclude that asymptomatic HIV elicits an "atypical" APR in which positive APP concentrations increase, but negative APP concentrations do not decline.34 Citing this work, authors of a meta-analysis on the effect of subclinical infection on serum retinol concentration suggested that HIV is unlikely to affect retinol concentration in population-based surveys, so that "…even where [HIV] infection is endemic, it can probably be overlooked, and the sample size needed to establish the prevalence of vitamin A deficiency can be calculated with the same disease and environmental factors as for other population groups."35 Because serum retinol among HIV-negative women responded to supplementation, whereas it did not among asymptomatic HIV-positive women in this population, we suggest the possibility that the capacity to maintain negative APP concentrations during asymptomatic HIV may be sensitive to dietary protein intake. Estimated per capita dietary protein intake in Zimbabwe is lower (54 g/d per capita, 10% of calories)36 compared with the HIV-positive subjects in the UK study (122 g/d, 20% of calories), and the percentage of protein from animal sources (i.e., protein quality) is 12% in Zimbabwe36 compared with 70% in western diets.37 Indeed, among asymptomatic HIV-positive men in Cote d'Ivoire, RBP and TTR concentrations were 20% significantly lower, whereas concentrations of 2 positive APPs (C-reactive protein and α-1 glycoprotein) were not higher compared with HIV-negative healthy controls.38 Our observation that supplementation increased, but did not normalize, serum retinol among HIV-positive women with CD4 count of less than 200 cells could be explained if dietary vitamin A was limiting relative to circulating RBP and TTR concentrations among these women who were more poorly nourished based on several measures of nutritional status (i.e., MUAC, body mass index, and Hb).
Simultaneous measurement of APPs during serum retinol surveys is recommended to adjust for the effects of acute infection.39-41 Further research is required to better understand vitamin A metabolism and the APR during asymptomatic HIV in populations ingesting marginal quantity and quality of dietary protein. Until this is better understood, we suggest that simultaneous HIV testing would also be informative in interpreting serum retinol in these populations and that sample sizes for surveys should be sufficient to estimate serum retinol for HIV status strata.
Vitamin A supplementation was associated with a decrease in sick clinic visits for malaria, cracked and bleeding nipples, pelvic inflammatory disease, and vaginal infection. In Nepal, weekly vitamin A supplementation reduced urinary and genital tract infections during late pregnancy,42 and in Papua New Guinea, periodic high-dose vitamin A supplementation reduced malarial morbidity among children.43
Our data underscore the enormous toll that HIV is taking on maternal health in Zimbabwe: among the women who were HIV positive at delivery, 7.5% died (38/1000 p-y) during the following 2 years. This is similar to rates observed in 2 studies of Rwandan antenatal clinic attendees (7% during 2 years29 and 50.1/1000 p-y).44 Our data corroborate the recently highlighted substantial mortality effect of coinfection with tuberculosis and HIV among women:9 in ZVITAMBO, tuberculosis was the most common cause of death, and nearly all tuberculosis-associated deaths were among HIV-positive women.
The risk of death among infected compared with uninfected women did not vary significantly with age. In contrast, the mortality rate ratio of infected to uninfected people in several large African population-based cohorts was 12 in teenagers and in people older than 30 years (similar to the excess risk we observed among all age groups of ZVITAMBO women) but was much higher (20-30) among people aged 25 to 30 years.45 The "flatter" relationship of mortality rate ratio to age among women in ZVITAMBO might be because they were immediate postpartum and so were selectively healthier than similarly aged HIV-positive people in cohorts representative of general populations. This relative health would be greatest among the 20- to 30-year age group because teenagers in both the population-based cohorts and ZVITAMBO would have been infected for a similarly short period, and underlying mortality rates among older HIV-negative adults are higher in Uganda, Tanzania, and Malawi compared with Zimbabwe.46
In another article, we report that, in ZVITAMBO, maternal vitamin A supplementation had no beneficial effects on infant HIV infection or overall mortality and that it had an adverse effect among a subgroup of infants: among HIV-exposed infants who escaped becoming infected by their mothers during pregnancy and delivery, maternal vitamin A supplementation increased their risk of death by age 2 years.18 The explanation offered was that priming with vitamin A may have increased viral load among babies who subsequently became infected during breast-feeding. Taken together with the lack of benefit on maternal mortality and limited benefit on maternal morbidity reported in the current article, this finding raises concern about universal postpartum maternal vitamin A supplementation in areas of HIV endemicity. However, at least 2 issues require further research to fully inform policy decisions about postpartum maternal supplementation in HIV endemic areas. First, if ongoing studies confirm the beneficial effect of frequent supplementation on maternal mortality which was observed in Nepal,4 then the effect of maternal vitamin A supplementation (perhaps given as smaller, more frequent doses) on maternal mortality should be reexamined in an HIV-endemic population that has poorer vitamin A status than the women who participated in ZVITAMBO. Second, among a small subsample of HIV-negative women in the ZVITAMBO trial for whom serum retinol was measured, those with low concentrations (<0.7 μmol/L) were 10.4 (95% CI, 3.0-36.3) times more likely to acquire HIV during the postpartum period, and supplementation of these apparently deficient women tended to reduce HIV incidence, although not significantly so (perhaps due to limited statistical power).47 Should this protection be confirmed and reach statistical significance, this benefit may override other issues related to postpartum maternal supplementation in populations in which vitamin A deficiency is widespread and HIV incidence is high.48
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