For women who started therapy during pregnancy, approximately one-third received combination therapy including a protease inhibitor, and this did not differ by race/ethnicity (Table 4). However, for women in this group who did not receive combination protease inhibitor therapy, black and Hispanic women were more likely to receive monotherapy (25% each for black and Hispanic) than were white women (12%), although this trend was not statistically significant. Women who were not receiving therapy prior to pregnancy most often cited “not knowing their HIV status” as the reason for not being on treatment; this occurred in 251 black (44%), 85 Hispanic (41%), and 36 white women (32%). Infant ARV treatment did not vary by racial/ethnic group (Table 3).
In the present analysis cohort, the incidence of elective cesarean delivery differed according to race/ethnicity (P = 0.04;Table 3) with black women having the lowest rate. This difference, however, was restricted to women with HIV RNA ≤400 copies/mL at delivery, who have a low risk of transmission; there was no difference in the rate of elective cesarean delivery by race/ethnicity for women with detectable HIV RNA levels at delivery (HIV RNA > 400) (P = 0.62).
Other factors potentially associated with transmission risk, including duration of membrane rupture, intrapartum maternal antibiotics (as a surrogate for potential chorioamnionitis), and fetal scalp electrode use, were compared by race/ethnicity among the 447 women with active viral replication (HIV RNA > 400 copies/mL) at the time of delivery. Among the 48 white women with HIV RNA > 400 copies/mL at delivery, mean and median duration of membrane rupture (8.0 hours and 1.6 hours, respectively) were longer than in the 308 black women (5.4 hours and 1.1 hours) and 91 Hispanic women (3.0 hours and 0 hours) (P = 0.002). Other intrapartum procedures and interventions including antibiotic administration and invasive fetal monitoring did not differ among racial/ethnicity groups.
In univariate analysis for modeling/predicting the probability of HIV transmission from mother to child in this subset of women in PACTG 316, the only significant predictor of HIV transmission (P < 0.05) was maternal HIV RNA at the time of delivery. In multivariate modeling, when including all variables satisfying P value ≤0.25 in the univariate model, race/ethnicity and CDC disease category did not improve the model (data not shown).
Because HIV RNA at delivery is the primary factor associated with transmission risk, we sought to determine which demographic and treatment/intervention variables would predict successful suppression of viral replication at delivery (ie, HIV RNA ≤ 400 copies/mL at delivery). Univariate analysis demonstrated that type of ARV therapy (combination with and without protease inhibitor, monotherapy, no ARV), time of initiation of ARV (before vs. during or after the second trimester), time of initiation of prenatal care (before or during third trimester), race (white vs. other race/ethnicity), mode of delivery (spontaneous vaginal vs. other mode) were individually significantly associated with successful viral suppression at delivery (Table 5). In bivariate models that included ARV type and individual variables that had values P ≤0.25 in univariate analysis, time of ARV initiation, time of prenatal care initiation, and race/ethnicity each retained significance in predicting successful viral suppression at delivery. Additionally, race/ethnicity remained predictive of successful viral suppression at delivery in a multivariate model incorporating all of these variables (type of ARV, time of ARV initiation, and time of prenatal care initiation) (P = 0.01).
The transmission rate of 1.5% in PACTG 316 is reflective of success achieved in developed countries in reducing perinatal HIV transmission. In this study, maternal HIV RNA level at delivery was the single most important factor in predicting risk of transmission, and the small number of infected infants (19 infants) limits the ability to evaluate other potential correlates of transmission risk and larger data sets may be needed to further evaluate the impact of race on perinatal HIV transmission. The differences in transmission by race/ethnicity observed in PACTG 316 were of concern to the study team and led to a further investigation of the relationship between race/ethnicity and other risk factors for transmission.
Because HIV RNA level is so critical in determining transmission, factors predicting successful suppression of viral replication at delivery, defined as delivery HIV RNA levels ≤400 copies/mL, were explored. A number of factors, including race/ethnicity, type of ARV therapy, and time in pregnancy when prenatal care was initiated were significant predictors of viral suppression at delivery. Time of initiation and type of ARV used as well as access into medical care might be expected to impact on HIV RNA; without access to ARV treatment, a decrease in viral load would not occur. However, race/ethnicity as an independent predictor of viral suppression during pregnancy has not been previously described.
Maternal disease status differed by race/ethnicity in PACTG 316, with pregnant white women having significantly higher entry CD4 cell counts and lower delivery HIV RNA levels than either black or Hispanic women. We found that a higher proportion of white women had started therapy prior to pregnancy compared with women of other race/ethnicity and that women who started therapy before pregnancy, regardless of race/ethnicity, were more likely to have received highly active antiretroviral therapy (HAART) with a protease inhibitor. Thus, the overall greater use of ARV combinations that included a protease inhibitor among white women appears to be attributable to a larger proportion of white women being on ARV therapy prior to pregnancy compared with the other race/ethnic groups.
The more advanced disease status, as demonstrated by lower CD4 counts, of the black and Hispanic women might be expected to lead to a greater use of HAART among those who started treatment during pregnancy; however, this is not what was observed. For women who initiated therapy during pregnancy, there were similar rates of HAART use in all 3 racial/ethnicity groups, although among women who did not receive a protease inhibitor, there was greater use of monotherapy in the black and Hispanic women. The present study is limited by the fact that clinical information such as CD4 count and HIV RNA was collected during the periods of study but was not available for times prior to pregnancy or earlier during pregnancy when many of the decisions about maternal ARV therapy were being made.
The most frequent reason cited by study subjects for not receiving ARV therapy prior to pregnancy was that they had not known their HIV status. In addition, there was a significantly longer interval from the time of diagnosis of HIV infection to delivery in white women compared with the black and Hispanic women in the cohort. These findings suggest that some of the differences in prescribing ARV medication between racial/ethnic groups may be due to a greater number of white women in the present study having their HIV infection diagnosed prior to pregnancy. The white women in this cohort have a high prevalence of illegal drug use and therefore may have been identified as being at risk for HIV. In contrast, black and Hispanic women in the present cohort were not likely to report illegal drug use and therefore may not have been perceived to be at risk for HIV infection. It is also possible that the black and Hispanic women in this study may have less access to health care or may be less likely to be offered or agree to an HIV test. These findings suggest that all women of childbearing age should be offered HIV testing when they access medical care, not just those with risk factors such as drug abuse or pregnancy.
In contrast to the difference in antenatal ARV therapy, the intrapartum approach to care for those women with detectable HIV RNA and for infants after delivery was identical for all racial/ethnic groups. Elective cesarean delivery has been shown to reduce the risk of perinatal transmission in both a clinical trial and meta-analysis. 6,7 In the overall study cohort there was less frequent use of elective cesarean delivery in black women. However, when restricted to women with delivery HIV RNA levels of >400 copies/mL—those for whom elective cesarean delivery would likely provide a benefit—there is no difference in incidence of elective cesarean among racial/ethnic groups.
Other investigators have described racial/ethnic differences in practices for treatment and other care in HIV-infected adults in the United States. 8,9 In a review of the literature, Palacio et al 10 reported racial/ethnic disparities in utilization of highly active ARV therapies. In studies of HIV-infected women who were not pregnant, factors such as care by an HIV specialist, maternal age >25 years, education, absence of drug use, and not being African American have been shown to predict increased use of highly active ARV combinations. 11–13
Differences by race/ethnicity in quality of care received or access to care have also been described for other medical conditions including congestive heart failure, pneumonia, and coronary bypass surgery. 14,15 We were not able to document a difference in highly active treatment administered in those treated prior to pregnancy or those starting therapy during pregnancy. Participants who reported combination therapy without a protease inhibitor were not receiving highly active treatment: in all cases they were receiving combinations of nucleoside analogues. In fact, stratifying the analysis according to when ARV therapy started relative to pregnancy (Table 4), the percent of women who received “highly active” protease inhibitor–containing combinations was virtually identical across racial/ethnic groups.
Even after accounting for type of ARV and time of initiation of ARV, race/ethnicity remained significant in predicting viral suppression at the time of delivery. Other investigators have not found a difference in response to ARV therapy related to race. Jensen-Fangel et al 16 reported identical rates of viral suppression and CD4 increases in whites and nonwhites in a Danish cohort with free access to medication. Further, in a cohort of US patients, race/ethnicity was not found to impact on CD4 response in patients with suppressed virus on HAART. 17 These reports suggest that the differences in viral suppression seen in this study are not related to genetic factors but may be related to treatment differences that were not measured in our study or to other differences including other infections, adherence, or pharmacokinetics. The present study was not designed to evaluate all of these factors.
The implications of higher RNA and lower CD4 cells in pregnant black and Hispanic women in this trial are important both for the woman's health and for her child's outcome. Further prospective studies should be designed to explore the factors that are considered when determining the type of ARV combinations initiated during pregnancy and whether race/ethnicity, language barriers, access to care, health care provider, or socioeconomic factors affect health care providers' recommendation for therapy or the patients' willingness to initiate therapy.
The International PACTG study team includes Brigitte Bazin (ANRS France), Yvonne Bryson (University of California School of Medicine, Los Angeles), Paula Britto (Statistical and Data Analysis Center, Harvard School of Public Health, Boston, MA), Mary Culnane and Scharla Estep (National Institute of Allergy and Infectious Diseases, Bethesda, MD), Jean Francois Delfraissy (ANRS France), Marie-Louise Newell and Simona Fiore (ECS Institute of Child Health, London England), Maria Gigliotti (Boehringer Ingelheim), Mark Mirochnick (Department of Pediatrics, Boston University School of Medicine, Boston, MA), Claire Rekacewicz (ANRS France), Maureen Shannon (San Francisco General Hospital, San Francisco, CA), and Heather Watts (National Institute of Child Health and Human Development, Bethesda, MD). Participating sites are listed in the primary publication from the study (Dorenbaum A, Cunningham CK, Gelber RD, et al. Two-dose intrapartum/newborn nevirapine and standard ARV therapy to reduce perinatal HIV-1 transmission: a randomized trial. JAMA. 2002;288:189–1998
). In addition to the site participants listed in the primary manuscript, one additional site also enrolled patients in this study: Donna Sandifer and Rene Smit at Children's National Medical Center, Washington, DC.
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Keywords:© 2004 Lippincott Williams & Wilkins, Inc.
race/ethnicity; perinatal HIV transmission; clinical trials