Exposure to Entry Inhibitors Alters HIV Infectiousness and Sensitivity to Broadly Neutralizing Monoclonal Antibodies

Kramer, Victor G. MSc*,†; Varsaneux, Olivia BSc*,‡; Oliviera, Maureen BSc*; Colby-Germinario, Susan P. MSc*; Mesplède, Thibault PhD*; Wainberg, Mark A. PhD*,†,‡

JAIDS Journal of Acquired Immune Deficiency Syndromes: 1 September 2014 - Volume 67 - Issue 1 - p 7–14
doi: 10.1097/QAI.0000000000000223
Basic and Translational Science

Background: The development of envelope-specific neutralizing antibodies that can interfere with viral entry into target cells is important for the development of an HIV-1 vaccine. Another means of blocking viral entry is through the use of entry inhibitors such as the CCR5 inhibitor maraviroc (MVC), which can also repel cell-free virus particles from the cell surface. For this reason, we hypothesized that exposure to entry inhibitors might alter viral infectiousness and sensitivity to antibody-mediated neutralization.

Methods: The CCR5-tropic HIV-1 variants BaL, AD8, and CC 1/85 were used to infect PM-1 cells in the presence of 2 entry inhibitors, enfuvirtide and MVC. After 4 hours, culture fluids were ultrafiltered and the infectiousness and susceptibility to broadly neutralizing antibodies (2F5, 4E10, 2G12, b12, VRC01, PG9) of viruses exposed to these entry inhibitors were assessed using TZM-bl cells.

Results: Viruses exposed to the entry inhibitor MVC exhibited lower infectiousness than controls. Enfuvirtide exposure increased AD8 sensitivity to 2F5, 4E10, VRC01, and b12 and increased BaL sensitivity to 4E10 while lowering BaL sensitivity to b12 and VRC01. MVC-exposed BaL became less susceptible to the gp120-specific antibodies b12, 2G12, and VRC01.

Conclusions: Exposure to entry inhibitors altered HIV-1 infectiousness and sensitivity to gp120-specific neutralizing antibodies. This alteration of entry inhibitor-exposed virus has implications for the development of future entry inhibitors and for vaccine development.

*McGill University AIDS Centre, Lady Davis Institute, Jewish General Hospital, Montreal, Quebec, Canada;

Division of Experimental Medicine, McGill University, Montreal, Quebec, Canada; and

Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.

Correspondence to: Mark A. Wainberg, PhD, McGill AIDS Centre, 3999 Côte-Sainte-Catherine Road, F-328, Montrea QC H3T 1E2 Canada (e-mail: mark.wainberg@mcgill.ca).

Supported by Canadian Institutes of Health Research. M.A.W. has received grant support from Pfizer, ViiV, Janssen and Merck, Inc.

The remaining authors have no funding or have no conflicts of interest to disclose.

Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Web site (www.jaids.com).

Received December 13, 2013

Accepted May 01, 2014

© 2014 by Lippincott Williams & Wilkins