Skip Navigation LinksHome > July 1, 2014 - Volume 66 - Issue 3 > Altered Innate Immune Development in HIV-Exposed Uninfected...
JAIDS Journal of Acquired Immune Deficiency Syndromes:
doi: 10.1097/QAI.0000000000000161
Basic and Translational Science

Altered Innate Immune Development in HIV-Exposed Uninfected Infants

Reikie, Brian A. MD*,†; Adams, Rozanne C. M. MSc; Leligdowicz, Aleksandra MD, PhD*; Ho, Kevin BSc*; Naidoo, Shalena MSc; Ruck, Candice E. BSc*; de Beer, Corena PhD§; Preiser, Wolfgang MD, PhD§; Cotton, Mark F. MD, PhD; Speert, David P. MD*; Esser, Monika MD; Kollmann, Tobias R. MD, PhD*

Supplemental Author Material
Collapse Box


Background: Early in life, HIV-exposed uninfected (HEU) infants are at an increased risk of morbidity and mortality from infectious disease compared with HIV-unexposed (UE) infants. To improve our understanding of the mechanisms underlying their increased risk, we contrasted innate immune development between HEU and UE infants in a developing world setting, where early life infectious disease risk is exceptionally high.

Methods: A prospective longitudinal cohort of HEU and UE newborns was established, and the most detailed characterization to date of HEU infant immune development was performed. Single-cell cytokine production was analyzed by flow cytometry after stimulation of whole blood with pathogen-associated molecular patterns (PAMPs).

Results: Monocyte, classical dendritic cell, and plasmacytoid dendritic cell composition was similar between HEU and UE infants throughout the first year of life. However, HEU mononuclear cells mounted an enhanced pro-inflammatory response to PAMP stimulation, both in quantity of cytokine produced per cell and in proportion of responder cells. Significant differences in cytokine production were detected on the single-cell level in a PAMP-specific pattern, but only at 2 and 6 weeks of age; all differences normalized by 12 months of age.

Conclusions: This time course of innate immune deviation early in life corresponds to the clinical window of vulnerability to infections in HEU infants and may be at least partially responsible for their increased morbidity and mortality from infectious disease.

© 2014 by Lippincott Williams & Wilkins


Article Tools


Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.