Institutional members access full text with Ovid®

Share this article on:

Diagnostic Accuracy of a Urine Lipoarabinomannan Enzyme-Linked Immunosorbent Assay for Screening Ambulatory HIV-Infected Persons for Tuberculosis

Gounder, Celine R MD, ScM; Kufa, Tendesayi MBChB, MPH; Wada, Nikolas I MHS; Mngomezulu, Victor MBBCh, FCRadDiag, MBA; Charalambous, Salome MBBCh, MSc; Hanifa, Yasmeen MRCGP, MSc; Fielding, Katherine PhD; Grant, Alison MBBS, PhD; Dorman, Susan MD; Chaisson, Richard E MD; Churchyard, Gavin J MBBCh, PhD

JAIDS Journal of Acquired Immune Deficiency Syndromes: October 1st, 2011 - Volume 58 - Issue 2 - p 219-223
doi: 10.1097/QAI.0b013e31822b75d4
Brief Report: Epidemiology and Prevention

Objective: To assess the diagnostic accuracy of the urine lipoarabinomannan (LAM) test among ambulatory HIV-infected persons.

Design: Cross-sectional.

Methods: HIV-infected persons consecutively presenting to the HIV Clinic at Tembisa Main Clinic in Ekhuruleni, South Africa, were screened for symptoms of tuberculosis (TB) and asked to provide sputum and blood samples for smears for acid-fast bacilli and mycobacterial culture and a urine specimen for a LAM enzyme-linked immunosorbent assay. Fine needle aspirates were obtained from participants with enlarged lymph nodes and sent for histopathology. Nonpregnant participants underwent chest x-ray.

Results: Four hundred twenty-two HIV-infected participants were enrolled with median age 37 years (interquartile range: 31-44 years), median CD4+ T-cell count 215 cells per microliter (interquartile range: 107-347 cells/μL), and 212 (50%) receiving antiretroviral therapy. Thirty (7%) had active TB: 18 with only pulmonary TB, 5 with only extrapulmonary TB, and 7 with both pulmonary TB and extrapulmonary TB. Twenty-seven percent [95% confidence interval (CI): 12% to 48%] of TB cases were sputum acid-fast bacilli positive. The sensitivity and specificity of the urine LAM compared with the gold standard of positive bacteriology or histopathology were 32% (95% CI: 16% to 52%) and 98% (95% CI: 96% to 99%), respectively. Urine LAM had higher sensitivity in TB cases with higher bacillary burdens, though these differences were not statistically significant.

Conclusions: The sensitivity of urine LAM testing is inadequate to replace mycobacterial culture. In contrast to prior research on the urine LAM, this study was conducted among less sick, ambulatory HIV-infected patients presenting for routine care.

From the *Center for TB Research, Division of Infectious Diseases, Department of Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD; †Aurum Institute for Health Research, Johannesburg, South Africa; ‡Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD; §Division of Diagnostic Radiology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; ‖Queen Mary, University of London, London, United Kingdom; ¶Department of Infectious Disease Epidemiology, Faculty of Epidemiology and Population Health; and #Department of Clinical Research, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom.

Received for publication April 28, 2011; accepted June 28, 2011.

Dr Celine Gounder was funded by National Institues of Health grant T32AI007291. This study was funded in part by a grant from the United States Agency for International Development (USAID).

All authors contributed to the conception and design of the study, interpretation of the data, and reviewing drafts of the article. T.K. was responsible for managing the field site. C.R.G. and N.I.W. analyzed the data. C.R.G. drafted and revised the article.

The authors have no conflict of interests to disclose.

The contents of this report are the sole responsibility of the authors and do not necessarily reflect the views of United States Agency for International Development or the United States Government.

Correspondence to: Dr Celine R. Gounder, MD, ScM, Center for Tuberculosis Research, Johns Hopkins University, CRB-2, Room M1.06, 1550 Orleans Street, Baltimore, MD 21287 (e-mail: gounder@jhmi.edu).

Copyright © 2011 Wolters Kluwer Health, Inc. All rights reserved.