Institutional members access full text with Ovid®

Share this article on:

Prevention of Mother-to-Child Transmission of HIV-1 Through Breastfeeding by Treating Mothers With Triple Antiretroviral Therapy in Dar es Salaam, Tanzania: The Mitra Plus Study

Kilewo, Charles MD*‡; Karlsson, Katarina MD, MA†‡; Ngarina, Matilda MD§; Massawe, Augustine MD*; Lyamuya, Eligius MD, PhD*; Swai, Andrew MD§; Lipyoga, Rosina MD§; Mhalu, Fred MD*; Biberfeld, Gunnel MD, PhD†‡ for the Mitra Plus Study Team

JAIDS Journal of Acquired Immune Deficiency Syndromes: November 2009 - Volume 52 - Issue 3 - p 406-416
doi: 10.1097/QAI.0b013e3181b323ff
Epidemiology and Social Science

Objective: The main aim of this study was to reduce breast-milk transmission of HIV-1 by treating HIV-1-infected women with highly active antiretroviral therapy (HAART) during breastfeeding.

Methods: Mitra Plus was an open-label, nonrandomized, prospective cohort study. HIV-1-infected pregnant women in Dar es Salaam were treated with zidovudine (ZDV) + lamivudine (3TC) + nevirapine (NVP). NVP was later replaced by nelfinavir for mothers with CD4 cell counts >200 cells per microliter or with adverse reaction to NVP. HAART was initiated at 34 weeks of gestation. For women with symptomatic HIV infection or CD4 cell counts below 200 cells per microliter, HAART was started earlier if possible. Treatment of the mothers was stopped at 6 months except for those mothers who needed HAART for their own health. The infants received ZDV + 3TC for 1 week after birth. Mothers were advised to exclusively breastfeed and to wean abruptly between 5 and 6 months. Transmission of HIV-1 was analyzed using the Kaplan-Meier survival technique. Cox regression was used for comparison with the breastfeeding population of the Petra trial arm A.

Results: There were 441 infants included in the analysis of HIV-1 transmission. The cumulative transmission of HIV-1 was 4.1 % [95% confidence interval (CI): 2.2 to 6.0] at 6 weeks, 5.0% (95% CI: 2.9 to 7.1) at 6 months, and 6.0% (95% CI: 3.7 to 8.3) at 18 months after delivery. The cumulative risk of HIV transmission between 6 weeks and 6 months was 1.0% and between 6 months and 18 months 1.1%. The cumulative HIV infection or death rate was 8.6% (95% CI: 6.0 to 11.2) at 6 months and 13.6% (95% CI: 10.3 to 16.9) at 18 months after delivery. Viral load at enrollment and duration of HAART before delivery were significantly associated with transmission but CD4 cell count at enrollment was not. The median time of breastfeeding was 24 weeks. The transmission in the Mitra Plus study was about half of the transmission in the breastfeeding population in the Petra trial arm A at 6 months after delivery (adjusted relative hazard = 0.49, P < 0.001). The combined outcome HIV infection or death was significantly lower in the Mitra Plus study than in the breastfeeding population in the Petra trial arm A at 18 months (adjusted relative hazard = 0.61, P = 0.007). NVP-related mucocutaneous rash was demonstrated in 6.5% of 429 NVP-exposed women. The incidence of NVP-related grade 3 or 4 hepatotoxicity was low (0.5%).

Conclusions: HAART given to HIV-infected mothers in late pregnancy and during breastfeeding resulted in a low postnatal HIV transmission similar to that previously demonstrated in the Mitra study in Dar es Salaam using infant prophylaxis with 3TC during breastfeeding. The extended maternal prophylaxis with HAART for prevention of mother-to-child transmission of HIV-1 for breastfeeding mothers who do not need HAART for their own health should be further evaluated and compared with the use of infant postnatal antiretroviral prophylaxis regarding safety and cost-effectiveness.

From the *Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania; †Swedish Institute for Infectious Disease Control (SMI), Stockholm, Sweden; ‡Department of Microbiology, Tumour, and Cell Biology, Karolinska Institutet, Stockholm, Sweden; and §Muhimbili National Hospital, Dar es Salaam, Tanzania.

Received for publication January 20, 2009; accepted June 12, 2009.

Supported by the Swedish International Development Agency, Department of Research Cooperation.

Parts of the data were presented at the Fourth International AIDS Society Conference on HIV Pathogenesis and Treatment, July 22-25, 2007, Sydney, Australia; at the Fourth Dominique Dormont International Conference: Host Pathogen Interactions in Chronic Infections, December 13-15, 2007, Paris, France; and at the International Conference on AIDS and sexually transmitted infections in Africa, December 3-7, 2008, Dakar, Senegal.

The members of the Mitra Plus Study Team are listed in Appendix 1.

Correspondence to: Gunnel Biberfeld, MD, PhD, Swedish Institute for Infectious Disease Control and Karolinska Institutet, SE-17182 Solna, Stockholm, Sweden (e-mail: gunnel.biberfeld@smi.se).

© 2009 Lippincott Williams & Wilkins, Inc.