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JAIDS Journal of Acquired Immune Deficiency Syndromes:
Brief Report: Epidemiology and Social Science

Longitudinal Assessment of HIV-1 and HSV-2 Shedding in the Genital Tract of West African Women

Nagot, Nicolas MD, MSc*†; Foulongne, Vincent PhD‡; Becquart, Pierre PhD‡; Mayaud, Philippe MD, MSc†; Konate, Issouf MD*; Ouedraogo, Abdoulaye MD*; Defer, Marie-Christine PhD*; Weiss, Helen PhD†; Van de Perre, Philippe MD, PhD‡; Segondy, Michel PhD‡

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Forty-two HSV-2-seropositive women, of whom 22 were HIV-1 seropositive, were followed up weekly for 3 weeks for assessment of HIV-1 and HSV-2 genital shedding in Bobo Dioulasso, Burkina Faso. HSV-2 DNA and HIV-1 RNA were quantitated in cervicovaginal lavages enriched with a cervical swabbing (eCVL) using real-time polymerase chain reaction. HIV-1 RNA shedding was detected in eCVL from 19 of 22 (86.4%) of the HIV-1-seropositive women and HSV-2 DNA was detected in eCVL from 12 of 42 (28.6%) of the women. Compared with the HIV-1-seronegative women, the HIV-1-seropositive women showed more persistent HSV-2 genital shedding and higher HSV-2 DNA loads in eCVL. This study showed that eCVL is a reliable sampling method for detecting both viruses. Three measurement points (at weekly intervals in this study) seem to adequately allow detection of most HIV-1 or HSV-2 genital shedders.

© 2005 Lippincott Williams & Wilkins, Inc.


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