Skip Navigation LinksHome > May 1, 2002 - Volume 30 - Issue 1 > gpl20-Independent Infection of CD4- Epithelial Cells and CD4...
Text sizing:
JAIDS Journal of Acquired Immune Deficiency Syndromes:
Artides: PDF Only

gpl20-Independent Infection of CD4- Epithelial Cells and CD4+ T-Cells by HIV-1.

Chow, Yen-Hung; Yu, Duan; Zhang, Jun-ying; Xie, Yiming; Wei, Lu-Chen O.; Chiu, Christopher; Foroohar, Mani; Yang, Otto O.; Park, No-Hee; Chen, Irvin S.Y.; Pang, Shen

Collapse Box


: Infection of CD4- cells by HIV-1 is well documented, but the mechanism responsible remains a matter of discussion. Previously we modified an HIV-1 virus strain, NL4-3, by deleting the Env proteins (gp41 and gp120) and inserting the enhanced green fluorescent protein (EGFP), and found that the Env(-) virus infects several types of CD4- cells. Here, we have prepared Env(-) virus from both the CD4" cell line, 293T, and the CD4+ cell lines, CEM and SUPT1, and found that HIV-1 Env(-) virus from either cell type is infectious for both CD4+ and several CD4- cell lines. Replication of HIV-1 Env(-) virus-infected cells was demonstrated by p24 gag protein assays and real-time reverse transcriptase polymerase chain reaction (RT-PCR) of the culture medium from infected cells. Virus collected from the HIV-1-Env(-) infected cultures proved infectious to several CD4- cell lines. Our results suggest that HIV-1 infects both CD4- and CD4+ cells using a gpl20-independent mechanism. This infection mechanism may provide new explanations for HIV-1 latency and persistent infection in patients.

(C) 2002 Lippincott Williams & Wilkins, Inc.


Article Tools


Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.