Dendritic Cells Transduced With HIV Nef Express Normal Levels of HLA-A and HLA-B Class I Molecules.

Cramer, Lorraine A.; Frelinger, Jeffrey A.
JAIDS Journal of Acquired Immune Deficiency Syndromes: August 15, 2001
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Summary: HIV Nef protein is important for viral pathogenesis and disease progression. Nef downregulates CD4 and major histocompatibility antigens on the surface of HIV-infected T cells. HIV also infects dendritic cells. We wanted to determine if Nef had a similar function in professional antigen-presenting cells, where downregulation of Class I could have important effects on the initiation of HIV specific cytolytic T cell responses. We infected human dendritic cells with adenovirus expressing Nef. In contrast to T cells and Hela cells, HLA-A and HLA-B molecules are not downregulated nor are other class I molecules increased. We show that, in dendritic cells, HIV Nef has little effect on CD4 or Class I expression.

(C) 2001 Lippincott Williams & Wilkins, Inc.