Chemokines Are Present in the Genital Tract of HIV-Seropositive and HIV-Seronegative Women: Correlation With Other Immune Mediators.

Spear, Gregory T.; Sha, Beverly E.; Saarloos, Mary Nel; Benson, Constance A.; Rydman, Robert; Massad, Stewart L.; Gilmore, Ruth; Landay, Alan L.
Journal of Acquired Immune Deficiency Syndromes & Human Retrovirology: August 15, 1998

: In this cross-sectional study, 53 cervicovaginal lavage samples (CVL) from 41 women were analyzed for the chemokines interleukin-8 (IL-8), regulated-onactivation normal T-expressed and secreted (RANTES) factor, and macrophage inflammatory protein-1[alpha] (MIP-1[alpha]) by enzyme-linked immunosorbent assay (ELISA). IL-8 was detected in 81% of CVL, whereas RANTES was detected in 32%, and MIP-1[alpha] in 15% of the CVL. The mean levels of IL-8, RANTES, and MIP-1[alpha] in positive samples were 396 pg/ml, 102 pg/ml, and 34 pg/ml, respectively. IL-8 levels correlated positively with IL-1[beta] and IgG in a subset of CVL samples. RANTES levels correlated positively with complement protein levels. Additionally, the levels of RANTES, but not MIP-1[alpha], reached levels reported in previous studies of the effects of [beta] chemokines to inhibit HIV replication. These results suggest that measuring chemokines in CVL specimens can provide important information regarding immune responses in the genital tract.

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