Skip Navigation LinksHome > April 1994 - Volume 7 - Issue 4 > Detection of Rectal Antibodies to HIV-1 by a Sensitive Chemi...
Journal of Acquired Immune Deficiency Syndromes:
Article: PDF Only

Detection of Rectal Antibodies to HIV-1 by a Sensitive Chemiluminescent Western Blot Immunodetection Method.

Mohamed, Omari Ali; Ashley, Rhoda; Goldstein, Andrew; McElrath, Julie; Dalessio, Julie; Corey, Lawrence

Collapse Box

Abstract

Summary: Western blot with a time-dependent enhanced chemiluminescence immunodetection method (ECL-WB) was shown to be 100-fold more sensitive than standard commercial colorimetric Western blots (WB) for detecting serum IgG to human immunodeficiency virus type I (HIV-1). ECL-WB was then used to test rectal secretions from 15 HIV-1 infected subjects (HIV +) and 7 uninfected subjects (HIV-) to document local IgG, IgA, and secretory component-associated immunoglobulin (SC-Ig) to HIV-1 proteins. Fourteen of 15 HIV+ subjects had rectal IgA to at least 1 HIV-1 protein, most often to gp41 (80%) or p24 (60%) and 14 (93%) had IgG to gp160, gp120, or gp41. Of seven HIV - subjects, none had detectable bands to HIV-1 proteins. SC-Ig to HIV-1 proteins was detected in all five rectal samples tested. However, the antibody profiles differed from those of rectal IgA, suggesting more than one source of rectal IgA to HIV. ECL-WB requires individual optimization and interpretation for each specimen as well as expensive reagents and is, therefore, not currently applicable to screening assays. However, the method offers promise as a sensitive method to characterize low-level immune responses (IgG, IgA, and SC-Ig) to HIV-1 proteins at local sites such as rectal mucosae.

(C) Lippincott-Raven Publishers.

Login

Article Level Metrics

Search for Similar Articles
You may search for similar articles that contain these same keywords or you may modify the keyword list to augment your search.